Diammineplatinum(II) nitrite

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

disregarded due to major methodological deficiencies

Study period:

not stated

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Not to current international guidelines. For example only two strains of bacteria used (should be five including Salmonella TA102 or E. coli according to OECD), no dose-response data reported, no controls used.

Data source

Reference

Reference Type:

publication

Title:

Mutagenic activity of some platinum and palladium complexes.

Author:

Uno Y. and Morita M.

Year:

1993

Bibliographic source:

Mutation Research, 298, 269-275.

Materials and methods

Principles of method if other than guideline:

Mutagenicity was tested using the Ames test, modified by the pre-incubation procedure.

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

cis-dinitrodiammineplatinum (II)

IUPAC Name:

cis-dinitrodiammineplatinum (II)

Constituent 2

Reference substance name:

cis-Pt(NH3)2(NO2)2

IUPAC Name:

cis-Pt(NH3)2(NO2)2

Details on test material:

- Name of test material (as cited in study report): cis-dinitrodiammineplatinum (II)

Method

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium TA 98

Species / strain 2

Species / strain / cell type:

S. typhimurium TA 100

Metabolic activation:

with and without

Metabolic activation system:

Liver S9 from Aroclor-1254 treated rats

Test concentrations with justification for top dose:

0.8-100 nmol/plate

Vehicle / solvent:

Distilled water

Controls

Untreated negative controls:

not specified

Positive controls:

not specified

Details on test system and experimental conditions:

The number of revertants per nmol was calculated from the number of revertants per plate and the molecular weight of the compound.

Tests were performed in triplicate.

Evaluation criteria:

By Ames' criteria a chemical will be considered mutagenic if, at the maximum non-inhibitory level, it more than doubles the number of spontaeous revertants

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium TA 98

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Test results 2

Species / strain:

S. typhimurium TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Additional information on results:

The frequency of revertants per nmol was not reported if a negative result was concluded by the authors.

Applicant's summary and conclusion

Conclusions:

Cis-dinitrodiammineplatinum (II) was non-mutagenic in a limited Ames test in two strains (TA98 and TA100) of Salmonella typhimurium, with and without S9

Executive summary:

In a limited published Ames test, the mutagenicity of cis-dinitrodiammineplatinum (II) was assessed in two strains (TA98 and TA100) only of Salmonella typhimurium bacteria, both in the presence and absence of S9 liver metabolic activation mix from Aroclor-1254 treated rats. The experiments were conducted in triplicate, at 0.8 - 100 nmol/plate. No control was used.

The test substance gave no evidence of mutagenicity, according to the investigators, in TA98 or TA100 when tested in the presence or absence of S9; the dose(s) tested was not clear.