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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Additional information on environmental fate and behaviour

Administrative data

Endpoint:
additional information on environmental fate and behaviour
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable scientific method though mechanism of fate processes not clearly identified in the study report (Therefore, fate process drawn from expert judgement)

Data source

Reference
Reference Type:
publication
Title:
The stabilities of various thiol compounds used in protein purifications
Author:
Stevens R, Stevens L and Priof NC
Year:
1983
Bibliographic source:
Biochemical education 11(2), 70.

Materials and methods

Test guideline
Qualifier:
no guideline followed
GLP compliance:
not specified
Type of study / information:
Abiotic degradation of several thiols.

Test material

Constituent 1
Chemical structure
Reference substance name:
2-mercaptoethanol
EC Number:
200-464-6
EC Name:
2-mercaptoethanol
Cas Number:
60-24-2
Molecular formula:
C2H6OS
IUPAC Name:
2-sulfanylethan-1-ol

Results and discussion

Any other information on results incl. tables


In summary, the half-live in hours (H) were influenced by the pH, temperature and metal ions (the by-products were not identified):

pH 6.5 20°C > 100 H
pH 7.5 20°C 10 H
pH 8.5 20°C 4 H

Temperature
pH 8.5 40°C 1 H
pH 8.5 20°C 4 H
pH 8.5 0°C 21 H

Metal ions: only Cu 2+ affects 2-ME stability (no effect of Fe3+, Cr3+, Ni2+, Fe2+, Mn2+, Zn2+, Ag+):
pH 8.5 20°C 4 H
pH 8.5 + 0.1 mM Cu2+ 20°C 0.6 H
pH 8.5 + 10 µM Cu2+ 20°C 1.5 H
pH 8.5 + 1.0 mM EDTA 20°C > 100 H
No significant effect recorded with 1.0 µM Cu2+.
Stability of TS increased by chelating agent EDTA.

Similar results with 0.01 M or 0.2 M potassium phosphate buffer or 0.1 M tris-HCl at the same pH.
Under the conditions used, loss of TS obeyed 1st-order kinetics.

Applicant's summary and conclusion