Registration Dossier
Registration Dossier
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EC number: 200-464-6 | CAS number: 60-24-2
Additional information on environmental fate and behaviour
Administrative data
- Endpoint:
- additional information on environmental fate and behaviour
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable scientific method though mechanism of fate processes not clearly identified in the study report (Therefore, fate process drawn from expert judgement)
Data source
Reference
- Reference Type:
- publication
- Title:
- The stabilities of various thiol compounds used in protein purifications
- Author:
- Stevens R, Stevens L and Priof NC
- Year:
- 1 983
- Bibliographic source:
- Biochemical education 11(2), 70.
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- GLP compliance:
- not specified
- Type of study / information:
- Abiotic degradation of several thiols.
Test material
- Reference substance name:
- 2-mercaptoethanol
- EC Number:
- 200-464-6
- EC Name:
- 2-mercaptoethanol
- Cas Number:
- 60-24-2
- Molecular formula:
- C2H6OS
- IUPAC Name:
- 2-sulfanylethan-1-ol
Constituent 1
Results and discussion
Any other information on results incl. tables
In summary, the half-live in hours (H) were influenced by the pH, temperature and metal ions (the by-products were not identified):
pH 6.5 20°C > 100 H
pH 7.5 20°C 10 H
pH 8.5 20°C 4 H
Temperature
pH 8.5 40°C 1 H
pH 8.5 20°C 4 H
pH 8.5 0°C 21 H
Metal ions: only Cu 2+ affects 2-ME stability (no effect of Fe3+, Cr3+, Ni2+, Fe2+, Mn2+, Zn2+, Ag+):
pH 8.5 20°C 4 H
pH 8.5 + 0.1 mM Cu2+ 20°C 0.6 H
pH 8.5 + 10 µM Cu2+ 20°C 1.5 H
pH 8.5 + 1.0 mM EDTA 20°C > 100 H
No significant effect recorded with 1.0 µM Cu2+.
Stability of TS increased by chelating agent EDTA.
Similar results with 0.01 M or 0.2 M potassium phosphate buffer or 0.1 M tris-HCl at the same pH.
Under the conditions used, loss of TS obeyed 1st-order kinetics.
Applicant's summary and conclusion
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