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Toxicity to soil microorganisms

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Description of key information

Nitrogen tranformation test (OECD 216): 28-d EC10 > 1000 mg/kg soil dw (BASF SE, 2013; project no. 37G0695/00X032)

Key value for chemical safety assessment

Additional information

A nitrogen transformation test according to OECD 216 was performed under GLP to determine the effects of the test substance to soil microorganisms (BASF SE, 2013; project no. 37G0695/00X032). The test medium was Lufa standard soil 2.3, a sandy loam which was uncultivated for at least 4 years and had received no pesticide or fertiliser treatment during that time). The test concentration series ranged from 62.5 to 1000 mg/kg soil dw. The nitrate content in the soil samples was determined on day 0 and day 28 to follow nitrate formation. Negative effects on the nitrate formation were not found, therefore no effect concentrations could be calculated. Nitrate concentration in treated soil was significantly higher than the nitrate concentration of the solvent control soil at concentrations of greater than or equal to 62.5 mg/kg soil dw on day 0 and greater than or equal to 125 mg/soil dw on day 28 of the test. The derived NOEC is based on the increase of nitrate in soil due to additional nitrate derived from the test substance: NOEC = 62.5 mg/kg soil, negative effects were lacking at the tested concentrations. It is assumed that the increase in nitrate content was due to the amount of nitrate entering the soil via the test substance.

A 28-d EC10 greater than 1000 mg/kg will be used for the environmental risk assessment.