trans-4-ethyl-4'-(4-propylcyclohexyl)-1,1'-biphenyl

Bioaccumulation: aquatic / sediment

Administrative data

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2010-09-29 to 2010-12-15

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: METI Guideline: Concentration Test on Chemical Substances in Fish

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

trans-4-ethyl-4'-(4-propylcyclohexyl)-1,1'-biphenyl

EC Number:

283-130-2

EC Name:

trans-4-ethyl-4'-(4-propylcyclohexyl)-1,1'-biphenyl

Cas Number:

84540-37-4

Molecular formula:

C23H30

IUPAC Name:

trans-4-ethyl-4'-(4-propylcyclohexyl)-1,1'-biphenyl

Specific details on test material used for the study:

Puritiy: > 99.94 %

Radiolabelling:

no

Sampling and analysis

Details on sampling:

- Sampling intervals/frequency for test organisms: days 0, 4, 7, 14, 21, 28
- Sampling intervals/frequency for test medium samples: days 0, 4, 7, 14, 21, 28
- Fish: two fish per sampling homogenised, and extracted with acetonitril/n-hexane. Further analysis performed by HPLC method with UV detection.

Test solutions

Vehicle:

yes

Details on preparation of test solutions, spiked fish food or sediment:

According to guideline

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): HCO-40
- Concentration of vehicle in test medium (stock solution and final test solution(s) at different concentrations and in control(s)): 10 mg/l

Dose 1: Test item: 0.001 mg/L ,
Dose 2: Test item: 0.0001 mg/L

Test organisms

Test organisms (species):

Cyprinus carpio

Details on test organisms:

Cyprinus carpio
Lot: 100215c
Weight: 7.5 g
Length: 8.5 cm

Study design

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

28 d

Total depuration duration:

0 d

Test conditions

Hardness:

250 mg/L

Test temperature:

25.0 ° +/- 0.4 °C

pH:

7.0

Dissolved oxygen:

saturated

Salinity:

According to guideline

Details on test conditions:

After acclimatisation period, the fish (Cyprinus carpio) were transferred into the test media under flow through conditions. Exposure concentrations were analytically verified at days 0, 4, 14, 21, and 28. The analytical values show that the nominal concentrations of 0.001 and 0.0001 mg/L were maintained during the experimental phase.
At days 0, 4, 14, 21, 25 and 28 two fish each were removed from each test group. Body weight was determined. The fish were homogenised and extracted with acetonitrile and n-hexane and the extract was analysed.

Due to the low bioaccumulation potential, no depuration phase was required.

Nominal and measured concentrations:

nominal concentration 0.0010 mg/L:
Solubilizer used: HCO-40, 10 mg/L

analytically measured concentrations:
day 4: 0.000918 mg/L
day 7: 0.000962 mg/L;
day 14: 0.000972 mg/L
day 21: 0.000970 mg/L
day 28: 0.000990 mg/L


nominal concentration 0.0001 mg/L
Solubilizer used: HCO-40, 10 g/L
analytically measured concentrations:
day 4: 0.000101 mg/L
day 7: 0.000102mg/L;
day 14: 0.000100 mg/L
day 21: 0.0000998mg/L
day 28: 0.0000988 mg/L

Reference substance (positive control):

no

Details on estimation of bioconcentration:

according to guideline

Results and discussion

Bioaccumulation factor

Bioaccumulation factor 1

Conc. / dose:

0.001 mg/L

Type:

BCF

Value:

> 35 - < 60 dimensionless

Basis:

normalised lipid fraction

Time of plateau:

14 d

Calculation basis:

steady state

Remarks on result:

other: HCO-40 used as solubilizer @10 mg/L

Bioaccumulation factor 2

Conc. / dose:

0 mg/L

Type:

BCF

Value:

> 26 - < 51 dimensionless

Basis:

normalised lipid fraction

Time of plateau:

14 d

Calculation basis:

steady state

Remarks on result:

other: HCO-40 used as solubilizer @10 mg/L

Depuration

Remarks on result:

other: mot performed due to low potential for bioaccumulation

Details on kinetic parameters:

steady state was reached within 14 days
Due to the low uptake, no depuration phase was necessary

Metabolites:

none

Details on results:

- Mortality of test organisms: no effects
- Behavioural abnormalities:no effects
- Observations on body length and weight:no effects
- Other biological observations: no effects

- Bound residues forming a plateau: no
- Mortality and/or behavioural abnormalities of control: no
- Loss of test substance during test period: no
- Results with vehicle control: no effects

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The test material showed no relevant bioaccumulation at concentrations at or above the water solubility. The steady state BCF values were at 45.8 and 36.4 (mean 41). The test material showed no relevant bioaccumulation (BCF < 2000).

Executive summary:

The study was performed according to the OECD Guideline for Testing of Chemicals 305 (Bioconcentration: Flow-through Fish Test) adopted on June 14, 1996 using two test material concentrations for exposure to Cyprinus carpio.

The test material showed no relevant bioaccumulation at concentrations at or above the water solubility. The steady state BCF values were at 45.8 and 36.4 (mean 41). The test material showed no relevant bioaccumulation (BCF < 2000).