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EC number: 260-686-4 | CAS number: 57345-19-4
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
Key value for chemical safety assessment
Additional information
The ready biodegradability of AMBERKETAL was determined by the Manometric Respiration Test according to OECD Guideline No 301F. Under the test conditions, biodegradation started after a long lag phase (19 days in one replicate, 24 days in the other replicate) reaching an average 38% biodegradation after 33 days. The extent of biodegradation achieved within 28 days was less than the pass level of 60% ThOD and as such AMBERKETAL cannot be considered as readily biodegradable.
The inherent biodegradability of AMBERKETAL was determined by the Manometric Respiration Test according to OECD Guideline No 302C. In this test, biodegradation started on day 6 and reached 70% on day 14, 86% on day 28 and 88% on day 31. When assessing inherent biodegradability it is not necessary to define limits on test duration. Biodegradation above 20% may be regarded as evidence of inherent, primary biodegradation, whereas biodegradation above 70% may be regarded as evidence of inherent, ultimate biodegradability. Thus, AMBERKETAL is regarded as inherently biodegradable according to this test.
The shorter lag phase in the inherent test probably reflects the higher concentration of inoculum used (100mg/L compared to 30mg/L in a 301F test) and, in turn, a larger biodiversity at the start of the test and a more rapid increase in the number of active degrading microorganisms before biodegradation becomes detectable above the background value of the inoculum. The bioavailability of the test item may also play a part. The test concentrations employed were 100mg (inherent test) and 30mg/L (ready test), which are significantly higher than the water solubility of AMBERKETAL, which is only 0.63 mg/L. This means that the biodegradation rate will be controlled by the dissolution rate. It follows, therefore that a more rapid biodegradation would be expected in tests performed at lower test item concentrations.
The high level of biodegradation observed in the inherent test (up to 88%) indicates that the substance is not expected to be persistent in the environment.
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