5',5'''-[ethylenebis(p-phenyleneazo)]bis[1',2'-dihydro-6'-hydroxy-4'-methyl-2'-oxo-1,3'-bipyridinium] dilactate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

From 7th to 17th May, 1991

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Justification for type of information:

The test substance is the acid form of the substance under registration; justification for Read Across is detailed in the IUCLID section 13.

Reason / purpose for cross-reference:

read-across source

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

not specified

Details on test solutions:

Test item was directly dispersed in water.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: rainbow trout
- Source: Parkwood Trout Farm, Kent, U.K.
- Length at study initiation: 4.4 ± 0.3 cm
- Weight at study initiation: 1.29 ± 0.27 g
- Maintenance of the brood fish:

ACCLIMATION
- Health during acclimation: moratlity lower than 1 % in the 7 days prior to the start of the test.

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Nominal and measured concentrations:

Range-finding: 1.0, 10 and 100 mg/l (nominal)
Main test: 100 mg/l (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: 20 litres
- Aeration: yes
- Renewal rate of test solution: daily
- N. organisms per replicate: in the range-finding test 2; in the main test 10.
- No. of vessels per concentration: in the range-finding test one replicate; in the main test two replicates.
- No. of vessels per control: one replicate.
- Biomass loading rate: 0.65 g bodyweight/litre

TEST MEDIUM / WATER PARAMETERS
- Dilution water: dechlorinated tap water (Sodium thiosulphate).
- Temperature: 14 °C
- Hardness: approximately 50 mg/l as CaCO3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Main test

Concentration (mg/l )	Cumulative mortality (initial population: 10)
	24 hrs	48 hrs	72 hrs	96 hrs
Control	0	0	0	0
Test item 100	0	0	0	0
Test item 100	0	0	0	0

Range-finding test

Concentration (mg/l )	Cumulative mortality (initial population: 2)
	24 hrs	48 hrs	72 hrs	96 hrs
Control	0	0	0	0
Test item 1.0	0	0	0	0
Test item 10	0	0	0	0
Test item 100	0	0	0	0

Conclusions:

LC50 (96h) > 100 mg/l (nominal)

Executive summary:

The short-term toxicity to fish of the test item has been investigated using Rainbow trout (Oncorhynchus mykjss) in semi-static system. A range-finding experiment was conducted at the nominal concentrations of 1.0, 10 and 100 mg/l. The main test was conducted at the nominal concentration of 100 mg/l; two replicates were assayed. Blank control was also been tested.

No deaths were recorded in both the range-finding and the main experiments.

Conclusion

LC50 (96h) > 100 mg/l (nominal)

Description of key information

Not harmful/toxic to fish. LC50 (96h) > 100 mg/l (nominal)

Additional information

There are no information about the short-term toxicity potential of Basic Yellow 094, thus the available information on the structural analogue Similar Substance 01 have been taken into consideration. Similar Substance 01 is the acid form of Basic Yellow 094; the read across approach can be considered as appropriate and suitable to assess the property under investigation (details about the approach are reported into the IUCLID section 13).

The short-term toxicity to fish of the Similar Substance 01 has been investigated using rainbow trout, under semi-static conditions. Based on the outcomes of a range-finding experiment, the main test was conducted at the nominal concentration of 100 mg/l. Under the tested conditions, no deaths were recorded in both the range-finding and the main experiments.

Although the concentrations were not measured, the substance is expected to be stable, over the entire testing period: on the basis of the chemical structure and functional groups presents, the substance is estimated to be water soluble and stable at possible natural pH conditions.