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A Salmonella typhimurium reverse mutation assay was performed with four histidine-requiring strains of Salmonella typhimurium (TA97, TA98, TA100, TA1535) according to current OECD guidelines, but not under GLP certified conditions. The test was performed in two independent experiments in the presence and absence of metabolic activation (rat or hamster liver S-9 mix induced by Aroclor 1254) with test concentrations up tp 10 mg/plate. Succinonitrile did not induce a significant dose-related increase in the number of relevant (His+) colonies in each of the four tester strains with or without metabolic activation. Based on the results of this study it is concluded that succinonitrile is not mutagenic in the Salmonella typhimurium reverse mutation assay with or without metabolic activation.

In a supporting study with several strains (Salmonella typhimurium spp., Escherichia coli spp. and Saccharomyces cerevisiae),

succinonitrile was not mutagenic when tested up to 500 ug/plate, with and without metabolic activation.

The mutagenicity of the chemicals of the cyanide family has been investigated in genemutation assays in bacteria and mammalian cell lines using a variety of methods to inducemetabolic activation. Cytotoxicity has been observed at the highest test concentrations. The studies of Hébert (1993), Monsanto (1983a) and Zeiger et al(1988) are considered to bethe key studies for gene mutation in bacteria; for mammalian cells this is the HPRT test by Godek (1983).Anin vitroAmes test with HCN inS. typhimuriumstrains TA1537, TA1538 and TA98 fordetection of frame shift mutation and TA1535 and TA100 for base-pair substitutions wasperformed with and without metabolic activation. There was no indication of mutagenicactivity of HCN under these conditions (Leuschner et al, 1983a cited by WHO, 1993).Chinese hamsters were orally dosed with HCN and preparations of metaphase cells were studied for structural chromosome aberrations. The incidence of aberrations or gaps was within the spontaneous range. Neither multiple aberrations nor pulverised metaphases were found. There was no indication of mutagenic properties relative to structural chromatid or chromosome damagein vivo(Leuschneret al, 1983b cited by WHO, 1993).

Short description of key information:

In a Salmonella typhimurium reverse mutation assay performed according to current OECD guidelines  but not under GLP certified conditions, succinonitrile was found to be not mutagenic with or without metabolic activation.

Endpoint Conclusion: No adverse effect observed (negative)

Justification for classification or non-classification

Based on the available data, succinonitrile does not have to be classified for mutagenicity according to CLP Regulation (EC) No. 1272/2008.