Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1991-12-12
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: non-GLP Guideline study, limited reporting

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1991
Report Date:
1991

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Lot No. 3/90 (24.04.1990)

Sampling and analysis

Analytical monitoring:
no

Test solutions

Details on test solutions:
500-1000 mg/L

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
Kommunale Abwasserreinigungsanlage Frankfurt-Sindlingen, non adapted sludge

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Post exposure observation period:
none

Test conditions

Hardness:
n.a.
Test temperature:
20 +/- 2 deg C
pH:
7.2 +/- 0.2
Dissolved oxygen:
saturated
Reference substance (positive control):
yes
Remarks:
3,5 Dichlorophenol 5-20-30 mg/L

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
NOEC
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The registration substance did not inhibit the respiration rate of microorganisms in activated sludge at the highest tested concentration of 1000 mg/L in an OECD 209 test. The NOEC (3h) is 1000 mg/L.