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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
15.08.2007 - 15.02.2008
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Qualifier:
according to guideline
Guideline:
other: OECD Series on Testing and Assessment, No. 23, "Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures", December 15, 2000.
Principles of method if other than guideline:
Commission Directive 92/69/EEC, Annex Part C, C.2: "Acute
Toxicity for Daphnia", Official Journal of the European
Communities No. L 383 A, dated December 29, 1992.

OECD Guideline for Testing of Chemicals 202: "Daphnia sp.,
Acute Immobilisation Test" adopted April 13, 2004.

OECD Series on Testing and Assessment, No. 23, "Guidance
Document on Aquatic Toxicity Testing of Difficult Substances
and Mixtures", December 15, 2000.
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Details on sampling:
One sample of the frshly prepared stock solution and duplicate samples from the freshly prepared test media of all test concentrations and the control were taken at the start of the test.
For the determination of the stability of the test item under the test conditions and of the maintenance of the test item concentrations during the test period, one sample of the stock solution and duplicate samples from the test media of all test concentrations and the control were collected at the end of the test (after 48 hours) by pouring together the contents of the test beakers of each treatment.
The concentrations of Y-15821 were analysed in the samples of the stock solution from both sampling times (0 and 48 hours). From the control samples only one of the duplicate samples was analysed from each of both sampling times.
Vehicle:
no
Details on test solutions:
Test Concentrations: 1.0; 0.45; 0.21; 0.094 and 0.043 mg test item/L and a control.
Control: In the control, culture medium was used without addition of the test item.
Dosage of the Test IteM: Before test start a concentrated stock solution was prepared by dissolving 50 mg test item in 500 ml of culture medium by intense stirring for 24 hours at room temperature to dissolve as much test item as possible (nominal
concentration: 100 mg test item/L). Then the following volumes of the stock solution were mixed with culture medium to result in a final volume of 600 ml obtaining the following test concentrations:
Amount of added stock solution [ml] Resulting nominal concentration [mg test item/L]
6.00 1.0
2.70 0.45
1.260 0.21
0.564 0.094
0.258 0.043
The test media were prepared just before introduction of Daphnia (=start of the test).
Test organisms (species):
Daphnia magna
Details on test organisms:
Species: Daphnia magna (Straus), clone 5
Age at test start: From 6.75 to 22.5 hours old
Sex: Female
Origin: ECT Oekotoxikologie GmbH, 65439 Flörsheim am Main, Germany.
Breeding Conditions: The Daphnia were bred in the laboratories of IBACON under similar temperature and light conditions as used in the test. The cultivation of the parental Daphnia was performed in reconstituted water of a similar quality with
regards to pH, the constituent salts and total hardness as the culture medium used in the test. The test organisms were not first brood progeny. Once a week the Daphnia in the stock culture were fed with Tetra Min-extract
and at least all other working days with green algae (Desmodesmus subspicatus) freshly grown in the laboratories of IBACON.
Reference Item: For the evaluation of the quality of the Daphnia clone used and the consistency of the experimental conditions, the reference item potassium dichromate p.A. was tested at least twice a year to demonstrate satisfactory test
conditions.
Acclimatisation: For 6.75 hours under test conditions.
Test type:
static
Water media type:
other: reconstituted water
Limit test:
no
Total exposure duration:
48 h
Hardness:
250 mg CaCO3/L
Test temperature:
19-20 °C
pH:
7.7 - 7.9
Dissolved oxygen:
8.3 - 8.7 mg/L
Nominal and measured concentrations:
During the test the daphnia were exposed to a mean of 81 % of nominal. Therefore, all reported results are related to nominal concentrations of the test item.
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
0.171 mg/L
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.074 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
1 mg/L
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
0.043 mg/L
Validity criteria fulfilled:
yes
Conclusions:
The toxic effect of the test item Y-15821 to Daphnia magna was assessed in a static dose-response test. The 48-hour EC50 value was 0.074 mg test item/L.
Executive summary:

The 48-hour EC50 value was 0.074 mg test item/L.

Description of key information

The toxic effect of the test item Y-15821 to Daphnia magna was assessed in a static dose-response test. The 48-hour EC50 value was 0.074 mg test item/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Dose descriptor:
EC50
Effect concentration:
0.074 mg/L

Additional information