4-benzoylbenzene-1,2,3-triyl tris(6-diazo-5,6-dihydro-5-oxonaphthalene-1-sulphonate)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Justification for type of information:

The information for this endpoint study record was obtained from an experimental study. The OECD GLP criteria were met and the methods were conducted according to laboratory practices commonly used in the year of the execution of the test. Hence, the data are considered reliable although they are not appropriate for current laboratory practices.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

Apr 04, 1984

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

Apr 25, 1984

Principles of method if other than guideline:

solubiliser 0,095 ml Tween 80/1 was applied during sample preparation

GLP compliance:

yes

Specific details on test material used for the study:

Name: Triester
Synonym: KF-KXU-64
Product No: RX CBEO 5
Purity: 65.1% Triester, 33.6 % Celite (CAS: 61790-53·2), 1.3 % Water
Charge: V4/87
CoA: 25.09.87
Appearance: yellow powder
Density: 330 g/L
pH. neutral
Solubility in water: practically insoluble

Analytical monitoring:

yes

Details on sampling:

samples were taken at 0, 48 and 96 hours

Vehicle:

yes

Remarks:

0,095 ml Tween 80/1

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Source: West Aquarium, 3422 Bad Lauterberg
Day of birth: 25.12.88
Delivery: 22.03.89
Acclimatization: 14 days

Test type:

static

Water media type:

freshwater

Remarks:

reconstituted water acc. to ISO/DIS 7346/1

Limit test:

yes

Total exposure duration:

96

Test temperature:

22.3 - 22.8°C

pH:

7.4 - 7.9

Dissolved oxygen:

7.2 - 8.8 mg/L

Nominal and measured concentrations:

500 mg/L (measured 454.7 mg/L, 90.9 %)

Key result

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

The vehicle (Tween 80) did not cause any effect and no mortality was observed during this study.

The test material did not cause any effect and no mortality was observed during this study.

Validity criteria fulfilled:

yes

Conclusions:

The EC50 (96 h) of the test material exceeds 500 mg/L.

Description of key information

The informtion for this endpoint study record was obtained from an experimental study. The OECD GLP criteria were met and the methods were conducted according to laboratory practices commonly used in the year of the execution of the test. Hence, the data are considered reliable although they are not appropriate for current laboratory practices.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

EC50

Effect concentration:

> 500 mg/L