4-benzoylbenzene-1,2,3-triyl tris(6-diazo-5,6-dihydro-5-oxonaphthalene-1-sulphonate)

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

07 Jan - 18 Feb 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

GLP compliance:

no

Type of method:

column elution method

Specific details on test material used for the study:

Storage conditions: Ambient temperature (10 to 30 °C), protected from light

Key result

Water solubility:

< 0.14 mg/L

Conc. based on:

test mat.

Temp.:

20 °C

Remarks on result:

other: solubility is below the quantification limit

Results
Preliminary visual estimation
Three preliminary tests were executed with specified test item amounts and 100 mL and 500 mL distilled water, respectively. After each addition of water, the mixture was stirred for at least 24 h and visually checked for undissolved particles.

Table 1 – Preliminary water solubility test results

Test item amount [mg]	Total volume [mL]	Mixture appearance
11.9	100	Not dissolved
11.7	500
4.4	500

 

The test item solubility in water was found to be below 8.8 mg/L (without correction for purity). The column elution method was used for the main test.

Column elution method
108.7 mg of test item were dissolved in 100 mL acetonitrile. For charging the columns in each case 25 mL of test item solution were added to ca.3 g of sea sand and the mixtures were shaken. The solvent was then removed under reduced pressure using a rotary evaporator (40 °C, ca. 20 mbar). The dry residues were placed into the columns of the test apparatus, which were then filled with water (test temperature: 20 °C, water pH at 23 °C: 5.3). After a swelling time of 2 h, the sand coated test item was rinsed with distilled water by pumps. At the end of the study, it was confirmed that residual test item remained on the columns, 50 mL acetonitrile was used to elute it. Throughout the study, the apparatus and test item solutions were protected from light.

Distilled water was used as a blank value, no signal was detected in the range of the test item retention time. Test item concentration was quantified by HPLC.

Column 12.5 mL/h
Rotation period of the fraction collector:90 min per fraction
Sampling duration: ~ 51 h (34 fractions)

Table 2 – Column elution method results

Fraction no.	V [mL]	pH	Water solubility [µg/L]
24	20.1	5.9	< 137
25
26
27
28

 

Column 25.0 mL/h
Rotation period of the fraction collector: 45 min per fraction
Sampling duration: ~ 50 h (67 fractions)

Table 3 – Column elution method results

Fraction no.	V [mL]	pH	Water solubility [µg/L]
53	17.4	6.8	< 137
54
55	17.2
56
57		6.7

 

Water solubility was found to be < 137 µg/L. In both columns, the concentration of the five consecutive fractions was constant within ± 30%. No increasing nor decreasing tendency was seen.

Conclusion: The water solubility of the test item at a temperature of 20 °C was determined to be below the quantification limit: < 0.14 mg/L

Conclusions:

The water solubility cs of the test item at a temperature of 20°C was determined according to the column elution method. It was found to be below the quantification limit: cs < 0.14 mg/L.

Executive summary:

The water solubility was determined according to OECD 105 using the column elution method. The preliminary test showed that the water solubility of the test item is < 10 mg/L. The column elution method showed a solubility: cs < 0.14 mg/L.

Description of key information

The water solubility cs of the test item at a temperature of 20°C was determined according to the column elution method and was found to be below the quantification limit, cs < 0.14 mg/L.