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Environmental fate & pathways

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Biodegradation in water:

Percent biodegradation of test chemical 4-[(5-amino-3-methyl-1-phenyl-1H-pyrazol-4-yl)azo]-2,5-dichlorobenzenesulphonic acid was observed to be  25 %  and 32 % by  test material analysis by HPLC parameter. On the basis of percent degradation it is concluded that this test material is inherently biodegradable.

Additional information

Biodegradation in water:

In a experimental study done from Chemosphere Vol 22 pp 107-119 1991 by Glenn M Shaul, Thomas J Holdsworth, Clyde R. Dempsey, and Kenneth A. Dostal in this study the biodegradability of test chemical 4-[(5-amino-3-methyl-1-phenyl-1H-pyrazol-4-yl)azo]-2,5-dichlorobenzenesulphonic acid (Acid yellow 49) was determined by analyzing percent recovery of test material. This test material is dye and inoculums used for biodegradation was obtained from Screened raw waste water from greater Cincinnati Mill Creek sewage treatment plant was used as influent (INF) to three pilot scale activated sludge biological treatment systems (Two experimental and one control). Each system consists of primary clarifier, complete mix aeration basin and secondary clarifier. Influent was spiked with test material at concentration of1 mg/L and 5 mg/L. Then Dye analytical studies were conducted by purified dye compound in organic free influent waste water and mixed liquor. Then influent, primary influent filtrate was passed through column packed with resin. The filter paper and resin were soaked in an ammonia – acetonitrile solution and then soxhlet extracted with ammonia – acetonitrile. The extract was concentrated and brought upto 50 ml volume with methanol/dimethyl formamaide solution. All extracted samples were analyzed by HPLC with ultraviolet detector.

 Percent recovery from mass balance data determined was 75 % in low spiked and 68 % in high spiked. On the basis of percent recovery value biodegradation of 4-[(5-amino-3-methyl-1-phenyl-1H-pyrazol-4-yl)azo]-2,5-dichlorobenzenesulphonic acid was 25% and 32 %by test material analysis by HPLC parameter. On the basis of percent degradation it is concluded that this test material is inherently biodegradable.