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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
secondary literature
Justification for type of information:
Data is from safety assessment report.

Data source

Reference
Reference Type:
secondary source
Title:
Fiber Reactive Dyes Toxicological Profiles December 1981
Author:
U.S. Consumer Product Safety Commission
Year:
1981
Bibliographic source:
Fiber Reactive Dyes Toxicological Profiles December 1981, PB83-168732

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
To assess eye irritation potential of Benzoic acid,2-((((3-((4-chloro-6-((4-((4-chloro-6-((3-sulfophenyl)amino)-1,3,5-triazin-2-yl)amino)-2 , 5-disulfophenyl)amino)- 1,3,5-triazin-2-yl) amino)-2-hydroxy- 5sulfophenyl)azo)phenylmethyl)azo)-5-sulfo-,pentasodium salt on rabbit eye
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid
Details on test material:
IUPAC: Benzoic acid, 2-((3-((4-chloro-6-((4-((4-chloro-6-((3-sulfophenyl)amino)-1,3,5-triazin-2-yl)amino)-2,5-disulfophenyl)amino)-1,3,5-triazin-2-yl)amino)-2-hydroxy-5 sulfophenyl)azo)phenylmethyl)azo)-5-sulfo-,pentasodium salt
common name: C.I. Reactive Blue 160
Molecular formula :C38H28Cl2N14O18S5.5Na
Molecular weight :1309.87
Substance type:organic
Physical state:solid
InChI:1S/C38H28Cl2N14O18S5.5Na/c39-33-45-35(41-18-7-4-8-19(11-18)73(58,59)60)49-36(46-33)42-24-15-29(77(70,71)72)25(16-28(24)76(67,68)69)43-37-47-34(40)48-38(50-37)44-26-13-21(75(64,65)66)14-27(30(26)55)52-54-31(17-5-2-1-3-6-17)53-51-23-10-9-20(74(61,62)63)12-22(23)32(56)57;;;;;/h1-16,31,55H,(H,56,57)(H,58,59,60)(H,61,62,63)(H,64,65,66)(H,67,68,69)(H,70,71,72)(H2,41,42,45,46,49)(H2,43,44,47,48,50);;;;;/q;5*+1/p-5/b53-51+,54-52+;;;;;
Smiles:[Na+].[Na+].[Na+].[Na+].[Na+].C(c1c(ccc(c1)S(=O)(=O)[O-])\N=N\[C@@H](c1ccccc1)\N=N\c1c(c(cc(c1)S(=O)(=O)[O-])Nc1nc(nc(n1)Cl)Nc1c(cc(c(c1)S(=O)(=O)[O-])Nc1nc(nc(n1)Cl)Nc1cc(ccc1)S(=O)(=O)O)S(=O)(=O)[O-])O)(=O)[O-]



Specific details on test material used for the study:
IUPAC: Benzoic acid, 2-((3-((4-chloro-6-((4-((4-chloro-6-((3-sulfophenyl)amino)-1,3,5-triazin-2-yl)amino)-2,5-disulfophenyl)amino)-1,3,5-triazin-2-yl)amino)-2-hydroxy-5 sulfophenyl)azo)phenylmethyl)azo)-5-sulfo-,pentasodium salt
common name: C.I. Reactive Blue 160
Molecular formula :C38H28Cl2N14O18S5.5Na
Molecular weight :1309.87
Substance type:organic
Physical state:solid
InChI:1S/C38H28Cl2N14O18S5.5Na/c39-33-45-35(41-18-7-4-8-19(11-18)73(58,59)60)49-36(46-33)42-24-15-29(77(70,71)72)25(16-28(24)76(67,68)69)43-37-47-34(40)48-38(50-37)44-26-13-21(75(64,65)66)14-27(30(26)55)52-54-31(17-5-2-1-3-6-17)53-51-23-10-9-20(74(61,62)63)12-22(23)32(56)57;;;;;/h1-16,31,55H,(H,56,57)(H,58,59,60)(H,61,62,63)(H,64,65,66)(H,67,68,69)(H,70,71,72)(H2,41,42,45,46,49)(H2,43,44,47,48,50);;;;;/q;5*+1/p-5/b53-51+,54-52+;;;;;
Smiles:[Na+].[Na+].[Na+].[Na+].[Na+].C(c1c(ccc(c1)S(=O)(=O)[O-])\N=N\[C@@H](c1ccccc1)\N=N\c1c(c(cc(c1)S(=O)(=O)[O-])Nc1nc(nc(n1)Cl)Nc1c(cc(c(c1)S(=O)(=O)[O-])Nc1nc(nc(n1)Cl)Nc1cc(ccc1)S(=O)(=O)O)S(=O)(=O)[O-])O)(=O)[O-]



Test animals / tissue source

Species:
rabbit
Strain:
not specified
Details on test animals or tissues and environmental conditions:
No data available

Test system

Vehicle:
not specified
Controls:
not specified
Amount / concentration applied:
No data available
Duration of treatment / exposure:
No data available
Observation period (in vivo):
No data available
Number of animals or in vitro replicates:
No data available
Details on study design:
No data available

Results and discussion

In vivo

Results
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: Not specified
Reversibility:
not specified
Remarks on result:
positive indication of irritation
Irritant / corrosive response data:
positive indication of ocular reactions were noticed

Applicant's summary and conclusion

Interpretation of results:
Category 2 (irritating to eyes) based on GHS criteria
Conclusions:
Eye irritation reactions were observed when Benzoic acid, 2-((((3-((4-chloro-6-((4-((4-chloro-6-((3-sulfophenyl)amino)-1,3,5-triazin-2-yl)amino)-2,5-disulfophenyl)amino)-1,3,5-triazin-2-yl)amino)-2-hydroxy-5 sulfophenyl)azo)phenylmethyl)azo)-5-sulfo-,pentasodium salt was installed on rabbit eyes.
Hence ,Benzoic acid, 2-((((3-((4-chloro-6-((4-((4-chloro-6-((3-sulfophenyl)amino)-1,3,5-triazin-2-yl)amino)-2,5-disulfophenyl)amino)-1,3,5-triazin-2-yl)amino)-2-hydroxy-5 sulfophenyl)azo)phenylmethyl)azo)-5-sulfo-,pentasodium salt was considered to be irritating to rabbit eyes.

Executive summary:

The ocular irritation potential of Benzoic acid, 2-((((3-((4-chloro-6-((4-((4-chloro-6-((3-sulfophenyl)amino)-1,3,5-triazin-2-yl)amino) -2,5-disulfophenyl)amino)-1,3,5-triazin-2-yl)amino)-2-hydroxy-5 sulfophenyl)azo)phenylmethyl)azo)-5-sulfo-,pentasodium salt on rabbit eye.

Positive ocular irritation reactions were observed. Hence, Benzoic acid, 2-((((3-((4-chloro-6-((4-((4-chloro-6-((3-sulfophenyl)amino)- 1,3,5-triazin-2-yl)amino)-2,5-disulfophenyl)amino)-1,3,5-triazin-2-yl)amino)-2-hydroxy-5 sulfophenyl)azo)phenylmethyl)azo)-5-sulfo-,pentasodium salt was considered to be irritating to rabbit eye.