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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
of read across substance
Justification for type of information:
Data for the target chemical is summarized based on the structurally similar read across chemicals
Reason / purpose:
read-across source
Reason / purpose:
read-across source
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
WoE report is based on two short term toxicity study of aquatic invertebrate for the test chemical :1.Short term toxicity of test chemical to aquatic invertebrates was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system.2.To evaluate short term toxicity of aquatic invertebrate
GLP compliance:
not specified
Specific details on test material used for the study:
Name of the test chemical: Sodium 4-{[4-(diethylamino)phenyl][4-(diethyliminio)-2,5-cyclohexadien-1-ylidene]methyl}-6-hydroxy-1,3-benzenedisulfonateCommon Name: Patent Blue V Sodium saltMolecular Formula: C27H32N2O7S2.NaMolecular Weight: 582.6709 g/molSMILES Notation: CCN(CC)C1=CC=C(C=C1)C(=C2C=CC(=[N+](CC)CC)C=C2)C3=CC(=C(C=C3S(=O)(=O)[O-])S(=O)(=O)[O-])O.[Na+]InChI: 1S/C27H32N2O7S2.Na/c1-5-28(6-2)21-13-9-19(10-14-21)27(20-11-15-22(16-12-20)29(7-3)8-4)23-17-24(30)26(38(34,35)36)18-25(23)37(31,32)33;/h9-18H,5-8H2,1-4H3,(H2-,30,31,32,33,34,35,36);/q;+1/p-1
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
other: 1. Daphnia magna 2.Brine shrimp
Details on test organisms:
- Common name: Water flea- Strain: Straus- Source: Own breeding at University of Chemistry and Technology, Prague- Age at study initiation (mean and range, SD): The animals used for the test shall be less than 24 h old and should not be first brood progeny- Feeding during test: No feeding
Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h
Test temperature:
20±1°C
pH:
1) sample: pH = 7.5 changed to pH = 7.8 during the test,control: pH = 7.8 did not change during the test
Dissolved oxygen:
1) higher than 8.9 mg/L at the end of test
Nominal and measured concentrations:
1) 200 mg/l
Details on test conditions:
1) TEST SYSTEM- Test vessel: 50 ml glass vessel- fill volume: 25 ml- No. of organisms per vessel: 5- No. of vessels per concentration (replicates): 4TEST MEDIUM / WATER PARAMETERS- Source/preparation of dilution water: Natural water (surface or ground water), reconstituted water or dechlorinated tap water are acceptable as culturing and dilution water if D. magna survives in it for the duration of the culturing, acclimation and testing without showing signs of stress. Waters in the range pH 6 to pH 9, with hardness between 140 mg/l and 275 mg/l (as CaCO3) are recommended.As an example, the preparation of dilution water meeting the requirements is described below.Dissolve known quantities of reagents in water. The dilution water prepared shall have a pH of 7.8 ± 0.5, a hardness of (225 ± 50) mg/l (expressed as CaCO3), a molar Ca + Mg ratio close to 4 + 1 and a dissolved oxygen concentration above 7 mg/l.Prepare the solutions specified below:- Calcium chloride solution: Dissolve 117.6 g of calcium chloride dihydrate (CaCl2.2H2O) in water (4.2) and make up to 1 l with water (4.2).- Magnesium sulfate solution: Dissolve 49.3 g of magnesium sulfate heptahydrate (MgSO4.7H2O) in water (4.2) and make up to 1 l with water (4.2).- Sodium bicarbonate solution: Dissolve 25.9 g of sodium bicarbonate (NaHCO3) in water (4.2) and make up to 1 l with water (4.2).- Potassium chloride solution: Dissolve 2.3 g of potassium chloride (KCI) in water (4.2) and make up to 1 l with water (4.2).MixingMix 2.5 ml of each of the four solutions and make up to 1 l with water.The dilution water shall be aerated until the dissolved oxygen concentration has reached saturation and the pH has stabilized. If necessary, adjust the pH to 7.8 ± 0.5 by adding sodium hydroxide (NaOH) solution or hydrochloric acid (HCI). The dilution water prepared in this way shall not be further aerated before use.- Sodium hydroxide solution, e.g. [NaOH] : 1 mol/l.- Hydrochloric acid, e.g. [HCl] : 1 mol/l.Reference substance: Dissolve 600 mg of potassium dichromate (K2Cr2O7) in water and make up to 1 l with water (4.2).OTHER TEST CONDITIONS- Adjustment of pH: no adjustment done- Photoperiod: No - Darkness- Light intensity:CALCULATION:EC50 was calculated using non linear regression by the software Prism 4.0
Reference substance (positive control):
yes
Remarks:
K2Cr2O7
Duration:
48 h
Dose descriptor:
other: inhibition percentage
Effect conc.:
4 other: %
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
792.86 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Validity criteria fulfilled:
not specified
Conclusions:
The test chemical Sodium 4-{[4-(diethylamino)phenyl][4-(diethyliminio)-2,5-cyclohexadien-1-ylidene]methyl}-6-hydroxy-1,3-benzenedisulfonate is not likely to be toxic to aquatic invertebrate atleast in the concentration range of 200-798 mg/l
Executive summary:

Data available for the structurally similar read across chemicals has been reviewed to determine the short term toxicity of aquatic invertebrate of the test chemical Sodium 4-{[4-(diethylamino)phenyl][4-(diethyliminio)-2,5-cyclohexadien-1-ylidene]methyl}-6-hydroxy-1,3-benzenedisulfonate (20262 -76 -4).The studies are as mentioned below:

1.Aim of this study was to assess the short term toxicity of test material to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.

 

The solution 200 mg/l was prepared by dissolving dark green powder in reconstituted water.200 mg/l nominal concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously.

The inhibition percentage for the test substance in Daphnia magna was determined to be 0.4% on the basis of mobility inhibition effects in a 48 hour study. Based on the inhibition percentage , indicates that the substance is likely to be non-hazardous to aquatic invertebrates and cannot be classified as aquatic as per the CLP criteria.

2.The effect concentration (EC50) value of test material in aquatic invertebrate [Brine shrimp] in a 48 hr study on the basis of mortality effect was found to be 792.86 mg/L. Based on this EC50 value, the substance does not qualify for the aquatic classification as per the  CLP criteria.

The test chemical Sodium 4-{[4-(diethylamino)phenyl][4-(diethyliminio)-2,5-cyclohexadien-1-ylidene]methyl}-6-hydroxy-1,3-benzenedisulfonate is not likely to be toxic to aquatic invertebrate  atleast in the concentration range of 200-798 mg/l. Hence, on the basis of above test concentration it can be considered that test material is non toxic to aquatic environment and can not be classified as per CLP classification

Description of key information

Short term toxicity to aquatic invertebrate:

Data available for the structurally similar read across chemicals has been reviewed to determine the short term toxicity of aquatic invertebrate of the test chemical Sodium 4-{[4-(diethylamino)phenyl][4-(diethyliminio)-2,5-cyclohexadien-1-ylidene]methyl}-6-hydroxy-1,3-benzenedisulfonate (20262 -76 -4).The studies are as mentioned below:

1.Aim of this study was to assess the short term toxicity of test material to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.

 

The solution 200 mg/l was prepared by dissolving dark green powder in reconstituted water.200 mg/l nominal concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously.

The inhibition percentage for the test substance in Daphnia magna was determined to be 0.4% on the basis of mobility inhibition effects in a 48 hour study. Based on the inhibition percentage , indicates that the substance is likely to be non-hazardous to aquatic invertebrates and cannot be classified as aquatic as per the CLP criteria.

2.The effect concentration (EC50) value of test material in aquatic invertebrate [Brine shrimp] in a 48 hr study on the basis of mortality effect was found to be 792.86 mg/L. Based on this EC50 value, the substance does not qualify for the aquatic classification as per the  CLP criteria.

The test chemical Sodium 4-{[4-(diethylamino)phenyl][4-(diethyliminio)-2,5-cyclohexadien-1-ylidene]methyl}-6-hydroxy-1,3-benzenedisulfonate is not likely to be toxic to aquatic invertebrate  atleast in the concentration range of 200-798 mg/l. Hence, on the basis of above test concentration it can be considered that test material is non toxic to aquatic environment and can not be classified as per CLP classification

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
200 mg/L

Additional information

Short term toxicity to aquatic invertebrate:

Data available for the structurally similar read across chemicals has been reviewed to determine the short term toxicity of aquatic invertebrate of the test chemical Sodium 4-{[4-(diethylamino)phenyl][4-(diethyliminio)-2,5-cyclohexadien-1-ylidene]methyl}-6-hydroxy-1,3-benzenedisulfonate (20262 -76 -4).The studies are as mentioned below:

1.Aim of this study was to assess the short term toxicity of test material to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.

 

The solution 200 mg/l was prepared by dissolving dark green powder in reconstituted water.200 mg/l nominal concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously.

The inhibition percentage for the test substance in Daphnia magna was determined to be 0.4% on the basis of mobility inhibition effects in a 48 hour study. Based on the inhibition percentage , indicates that the substance is likely to be non-hazardous to aquatic invertebrates and cannot be classified as aquatic as per the CLP criteria.

2.The effect concentration (EC50) value of test material in aquatic invertebrate [Brine shrimp] in a 48 hr study on the basis of mortality effect was found to be 792.86 mg/L. Based on this EC50 value, the substance does not qualify for the aquatic classification as per the  CLP criteria.

The test chemical Sodium 4-{[4-(diethylamino)phenyl][4-(diethyliminio)-2,5-cyclohexadien-1-ylidene]methyl}-6-hydroxy-1,3-benzenedisulfonate is not likely to be toxic to aquatic invertebrate  atleast in the concentration range of 200-798 mg/l. Hence, on the basis of above test concentration it can be considered that test material is non toxic to aquatic environment and can not be classified as per CLP classification