Registration Dossier

Administrative data

Endpoint:
pH
Type of information:
experimental study
Adequacy of study:
key study
Study period:
16-04-2018 – 16-04-2018
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed using standard test guidelines

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018
Report Date:
2018

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: OECD 122
Principles of method if other than guideline:
The pH determination of test item Hydrogen [4-[4-(diethylamino)-5'-hydroxy-2',4'- disulphonatobenzhydrylidene] cyclohexa-2,5-dien-1-ylidene]diethylammonium, monosodium salt (CAS No. 20262-76-4 ) was done as per the test guideline OECD 122. The mean pH of the test solution was determined with the help of digital pH meter.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid
Details on test material:
Name of the test chemical: Sodium 4-{[4-(diethylamino)phenyl][4-(diethyliminio)-2,5-cyclohexadien-1-ylidene]methyl}-6-hydroxy-1,3-benzenedisulfonateCommon Name: Patent Blue V Sodium saltMolecular Formula: C27H32N2O7S2.NaMolecular Weight: 582.6709 g/molSMILES Notation: CCN(CC)C1=CC=C(C=C1)C(=C2C=CC(=[N+](CC)CC)C=C2)C3=CC(=C(C=C3S(=O)(=O)[O-])S(=O)(=O)[O-])O.[Na+]InChI: 1S/C27H32N2O7S2.Na/c1-5-28(6-2)21-13-9-19(10-14-21)27(20-11-15-22(16-12-20)29(7-3)8-4)23-17-24(30)26(38(34,35)36)18-25(23)37(31,32)33;/h9-18H,5-8H2,1-4H3,(H2-,30,31,32,33,34,35,36);/q;+1/p-1Substance Type: OrganicPhysical State: Solid
Specific details on test material used for the study:
Name of the test chemical: Sodium 4-{[4-(diethylamino)phenyl][4-(diethyliminio)-2,5-cyclohexadien-1-ylidene]methyl}-6-hydroxy-1,3-benzenedisulfonateCommon Name: Patent Blue V Sodium saltMolecular Formula: C27H32N2O7S2.NaMolecular Weight: 582.6709 g/molSMILES Notation: CCN(CC)C1=CC=C(C=C1)C(=C2C=CC(=[N+](CC)CC)C=C2)C3=CC(=C(C=C3S(=O)(=O)[O-])S(=O)(=O)[O-])O.[Na+]InChI: 1S/C27H32N2O7S2.Na/c1-5-28(6-2)21-13-9-19(10-14-21)27(20-11-15-22(16-12-20)29(7-3)8-4)23-17-24(30)26(38(34,35)36)18-25(23)37(31,32)33;/h9-18H,5-8H2,1-4H3,(H2-,30,31,32,33,34,35,36);/q;+1/p-1Substance Type: OrganicPhysical State: Solid

Results and discussion

pH value
Key result
pH value:
5.6
Temp.:
26.2 °C
Concentration:
1 vol%
Remarks on result:
other: No other detail available

Applicant's summary and conclusion

Conclusions:
The pH determination of test item Hydrogen [4-[4-(diethylamino)-5'-hydroxy-2',4'- disulphonatobenzhydrylidene]cyclohexa -2,5-dien-1-ylidene]diethylammonium, monosodium salt (CAS No 20262-76-4) was done following OECD guideline 122. pH of 1% w/v solution was measured with the help of pH meter and it was observed to be 5.60 at 26.2 °C.
Executive summary:

The pH determination of test item Hydrogen [4-[4-(diethylamino)-5'-hydroxy-2',4'- disulphonatobenzhydrylidene]cyclohexa -2,5-dien-1-ylidene]diethylammonium, monosodium salt (CAS No. 20262-76-4 ) was done as per the test guideline OECD 122. The mean pH of the test solution was determined with the help of digital pH meter. The mean pH was determine to be 5.60 at 26.2°C.