Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 807-619-2 | CAS number: 65535-29-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1992
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
- Remarks:
- Draft report
- Analytical monitoring:
- yes
- Details on sampling:
- Temperature, pH- and oxygen values were measured at the beginning (0 h) and end of the test (48 h).
- Vehicle:
- yes
- Remarks:
- triethylene glycol
- Details on test solutions:
- Preparation of the stock solution and the test concentration:
- 11, 25 and 50 mg of test item were dissolved each in 10 mL of triethylene glycol; from this solutions 500 µL triethylene glycol were dissolved in 500 mL of tap water to prepare nominal concentrations (1.0, 2.3 and 4.5 g/L)
- For the control group 500 µL triethelene glycol were dissolved in 500 mL tap water
- Therefore, the concentration of triethylene glycol in the test concentration and in the control group was 1.13 g/L (specific gravity: 1.13 g/mL) - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Age: parthenogenetic females
- Source: Schering AG, Experimental Toxicology
- Age: 1-24 hours
- Maintenance and Acclimatisation: Parent animals were kept in glass vessels in water of the same quality and temperature as under experimental conditions
- Water: Normal tap water was used as dilution water, not polluted with harmful concentrations of chlorine, heavy metals or other substances, based on quarterly measurements and in accordance with the "Trinkwasserverordnung" (Lundt and Schiwy, 1986) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- The hardness of the dilution water used was 14.2 °dH (253 mg/L CaCO3)
- Test temperature:
- 20.8 °C (after 48 h) concerning the highest test concentration
- pH:
- 8.7 (after 48 h) concerning the highest test concentration
- Dissolved oxygen:
- 8.6 mg/L (after 48 hrs) concerning the highest test concentration
- Salinity:
- n.a.
- Conductivity:
- No data
- Nominal and measured concentrations:
- The analytical results showed a mean concentration of 0.2 mg/L (nominal 1.0 mg/L), 1.0 mg/L (nominal 2.3 mg/L) and 1.4 mg/L (nominal 4.5 mg/L).
- Details on test conditions:
- EXPOSURE CONDITIONS:
- Test vessels: 100 mL glass beakers covered with watch glasses holding 5 neonates in 50 mL of the test medium
- Experimental design: 3 test concentration plus 1 control; 5 neonates per vessel, 4 replicates per concentration/control
- Method of initiation: neonates were placed in prepared media
- Photoperiod: 12 h light : 12 h dark
- Temperature of incubation unit: 20.0 to 21.3 °C
- Aeration: none
- Test item concentrations: 1.0, 2.3 and 4.5 mg/L
- Method of administration: stock solution
- Medium renewal: none
- Criteria of effects: immobilization and visual abnormalities of the neonates, observed at 24 and 48 hours - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 4.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The test solution with the nominal concentration of 1.0 mg/L of the test substance had no adverse effects on the mobility of Daphnia. At a nominal concentration of 2.3 and 4.5 mg/L a immobilization rate of 15% (after 48 hours) was noted. One and two immobilized Daphnia in the control groups of the test is in the accepted range in the OECD guideline (10%). Therefore, it can be concluded that the EC50 of the test item was higher than 4.5 mg/L nominal, equivalent to 1.4 mg/L measured. The results indicate further that at this maximum concentration tested, there is a slight compound-related effect on the immobilization rate.
- Validity criteria fulfilled:
- yes
- Remarks:
- Immobilisation and other abnormalities in the controls did not exceed 10% by the end of the test; dissolved oxygen concentration remained above 3 mg/L throughout the exposure period
- Conclusions:
- After 48 hours of exposure conditions the EC50 to Daphnia magna was higher than 4.5 mg/L (equivalent to 1.4 mg/L measured). The result is expressed in terms of nominal concentration.
- Executive summary:
The study was conducted in agreement with the test guideline of the OECD, no. 202, "Daphnia spec., acute immobilization test"; adopted: 4. April 1984, and the EEC Directive 79/831, Annex V, Part C2: Acute toxicity tor Daphnia (draft) , Brussels 1989.
Twenty test organisms of Daphnia magna were used for each test concentration and for the control group. The Daphnia were exposed to the test concentrations and the dilution water for aperiod of 48 hours under static conditkms. Immobilization was recorded at 24 hours and 48 hours. The test temperature was in the range of 20.0 to 21.3 °C The pH of the test concentration and the control group was 7.7 -8.8, the oxygen concentration was between 8.2 and 9.5 mg/L. The light/dark rhythm was adjusted to 12 hours/12 hours. The nominal test concentrations were 1.0, 2.3 and 4.5 mg/L in three tests. For the concentration analysis samples were taken from each test vessel at 0, 24 and 48 hours. The concentrations were 0.23 mg/L for nominal 1.0 mg/L, 0.96 mg/L for nominal 2.3 mg/L and 1.39 mg/L for nominal 4.5 mg/L. There was a large variation between samples from one concentration. In the highest concentration tested, up to 15% of the test organisms were immobilized, simila!' to the medium concentration. At the lowest concentration, no immobilized Daphnia were observed. Also some immobilized Daphnia were observed in the control groups (up to 10%,). An immobilizaüon of 10% is within the limits recommended by the OECD guideline for organisms in the control group. Accordingly there seems to be a slight exposure related effect at the concentrations of 2.3 and 4.5 mg/L. After 48 hours of exposure conditions the EC50 to Daphnia magna was higher than 4.5 mg/L (equivalent to 1.4 mg/L measured).
This toxicity study is classified as acceptable and satisfies the guideline requirements for the acute Daphnia study.
Reference
Description of key information
After 48 hours of exposure conditions the EC50 to Daphnia magna was higher than 4.5 mg/L (equivalent to 1.4 mg/L measured). The result is expressed in terms of nominal concentration.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- > 4.5 mg/L
Additional information
An immobilization of 10% was within the limits recommended by the OECD guideline for organisms in the control group. Accordingly there seems to be a slight exposure related effect at the concentrations of 2.3 and 4.5 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.