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EC number: 268-697-6 | CAS number: 68133-69-7
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
ErC50 (72h) = 63.7 % of saturated solution (nominal loading 100 mg/l)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 63.7 mg/L
- EC10 or NOEC for freshwater algae:
- 28.7 mg/L
Additional information
No information on toxicity to aquatic algae and cyanobacteria was available on target substance. Therefore, available data on Similar Substance 01 was taken into account for the assessment. More details about the similarity between the two substances are reported in section 13.
Toxicity of test substance to unicellular freshwater green alga Pseudokirchneriella subcapitata was determined according to OECD guideline 201. The study was conducted under static conditions with an initial cell density of 6839 cells/ml. A saturated solution with a nominal loading of 100 mg test substance/l was prepared; undissolved particles were removed by membrane filtration. Thereafter, components of dilution water were added to saturated solution. Test concentrations obtained by dilution of saturated solution were: 6.25 - 12.5 - 25.0 - 50.0 - 100 % and were tested in triplicates. As for control, 6 replicates were used.
As test concentrations, measured by HPLC-DAD, resulted to be below LOD of 0.04 mg/l at start of exposure, no analysis at the end of exposure was done.
Based on 8 %, 32 % and 100 % inhibition of growth rate at concentration levels of 25 %, 50 % and 100 % of saturated solution after 72 h of exposure, an ErL50 of 63.7 % was established.
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