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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1976
Report Date:
1976

Materials and methods

Principles of method if other than guideline:
according to BASF-internal standard
GLP compliance:
no
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid
Details on test material:
Name of the test substance used in the study report: Aethyldiglykolacrylat

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals and environmental conditions:
Mean body weight: males 223 g, females 167 g

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
CMC (carboxymethyl cellulose)
Details on oral exposure:
0.5% aqueous CMC preparation and 2 - 3 drops Cremophor EL
Form of administration: emulsion
Concentrations used: 5.62, 6.81, 8.25, 10, 12.1, 14.7 and 17.8% (w/v)
Doses:
562, 681, 825, 1000, 1210, 1470 and 1780 µl/kg
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
ca. 1 106 mg/kg bw
Remarks on result:
other: original value: LD50 ca. 1100 µl/kg
Mortality:
562 and 681 µl/kg: no deaths; 825 µl/kg: 1/10 after 14 days; 1000 µl/kg: 3/10 after 14 days; 1210 µl/kg: 9/10 after 14 days; 1470 µl/kg: 9/10 after 14 days; 1780 µl/kg: 10/10 after 14 days
Clinical signs:
Dyspnea, apathy, partly abdominal position, staggering, atony, biting spasms, cyanosis, partly reddish feces and reddish-crusted noses (Ames blood test positive), poor general state
Body weight:
Initial partly body weight loss
Gross pathology:
Heart: acute dilatation; acute congestive hyperemia; glandular stomach: mucosa diffus reddened; proventriculus: border thickening, purulent, fibrinous plaque; slight diverticula formation; adherences with diaphragm; indifferent margins of the liver

Any other information on results incl. tables

Mortality:

Dose (µl/kg)

Conc. (%)

Number of animals

Died within

1 h

24 h

48 h

7 d

14 d

1780

17.8

5 male

0/5

5/5

5/5

5/5

5/5

5 female

0/5

5/5

5/5

5/5

5/5

1470

14.7

5 male

0/5

4/5

4/5

4/5

4/5

5 female

0/5

5/5

5/5

5/5

5/5

1210

12.1

5 male

0/5

5/5

5/5

5/5

5/5

5 female

0/5

4/5

4/5

4/5

4/5

1000

10.0

5 male

0/5

3/5

3/5

3/5

3/5

5 female

0/5

0/5

0/5

0/5

0/5

825

8.25

5 male

0/5

1/5

1/5

1/5

1/5

5 female

0/5

0/5

0/5

0/5

0/5

681

6.81

5 male

0/5

0/5

0/5

0/5

0/5

5 female

0/5

0/5

0/5

0/5

0/5

562

5.62

5 male

0/5

0/5

0/5

0/5

0/5

5 female

0/5

0/5

0/5

0/5

0/5

Applicant's summary and conclusion

Interpretation of results:
Category 4 based on GHS criteria
Conclusions:
The LD50 was ca. 1106 mg/kg bw.