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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
28.03.2017-24.05.2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Version / remarks:
OECD Guidelines for Testing of Chemicals (1992) No. 301B "Ready Biodegradability; CO2 Evolution Test"
Qualifier:
according to
Guideline:
EPA OPPTS 835.3110 (Ready Biodegradability)
Version / remarks:
paragraph m
Qualifier:
according to
Guideline:
other: Method C.4-C of Commission Regulation (EC) No. 440/2008
GLP compliance:
yes (incl. certificate)
Specific details on test material used for the study:
Identification: Ethoxy ethoxy ethyl acrylate CAS No. 7328-17-8
Physical state/Appearance:clear colorless liquid
Lot: 161225177
Purity: 96.20%
Expiry Date: 24 December 2017
Storage Conditions: room temperature in the dark
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
A mixed population of activated sewage sludge micro-organisms was obtained on 24 April 2017 from the aeration stage of the Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, UK, which treats predominantly domestic sewage.
Duration of test (contact time):
29 d
Initial conc.:
10 mg/L
Parameter followed for biodegradation estimation:
CO2 evolution
Reference substance:
benzoic acid, sodium salt
Key result
Parameter:
% degradation (CO2 evolution)
Value:
11
Sampling time:
2 d
Key result
Parameter:
% degradation (CO2 evolution)
Value:
45
Sampling time:
6 d
Key result
Parameter:
% degradation (CO2 evolution)
Value:
72
Sampling time:
8 d
Key result
Parameter:
% degradation (CO2 evolution)
Value:
78
Sampling time:
10 d
Key result
Parameter:
% degradation (CO2 evolution)
Value:
87
Sampling time:
14 d
Key result
Parameter:
% degradation (CO2 evolution)
Value:
103
Sampling time:
21 d
Key result
Parameter:
% degradation (CO2 evolution)
Value:
98
Sampling time:
28 d
Key result
Parameter:
% degradation (CO2 evolution)
Value:
104
Sampling time:
29 d
Results with reference substance:
Biodegradation: 90% after 28 days
Biodegradation: 97% after 29 days
Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
The test item attained 104% biodegradation after 28 days and satisfied the 10-Day window validation criterion, whereby 60% biodegradation must be attained within 10 days of the biodegradation exceeding 10%. The test item can therefore be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No. 301B.
The test item is considered as readily biodegradable.
Executive summary:

A study was performed to assess the ready biodegradability of the test item in an aerobic aqueous medium. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (1992) No. 301B, "Ready Biodegradability; CO2 Evolution Test" referenced as Method C.4-C of Commission Regulation (EC) No. 440/2008 and US EPA Fate, Transport, and Transformation Test Guidelines OCSPP 835.3110 (Paragraph (m)).

The test item attained 104% biodegradation after 28 days and satisfied the 10-Day window validation criterion, whereby 60% biodegradation must be attained within 10 days of the biodegradation exceeding 10%. The test item can therefore be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No. 301B.

The test item is therefore considered as readily biodegradable.

Description of key information

The test item attained 104% biodegradation after 28 days and satisfied the 10-Day window validation criterion, whereby 60% biodegradation must be attained within 10 days of the biodegradation exceeding 10%. The test item can therefore be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No. 301B.

The test item is therefore considered as readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information