Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1986
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: test performed in 1986, when shorter incubation periods were used

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1986
Report Date:
1986

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: DIN 38412, part 11
Deviations:
not specified
Principles of method if other than guideline:
The test guideline is similar to OECD test guideline 202. The incubation period was 24h, which was the standard incubation time at the time, when the test was performed (instead of 48 h according to current OECD 202).
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Purity: >98 %
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
not applicable

Sampling and analysis

Analytical monitoring:
not specified
Details on sampling:
no data

Test solutions

Vehicle:
no
Details on test solutions:
A stock solution was prepared. Initial weight was 10 g/L. This stock solution was filtered and the saturated solution diluted to the different test concentrations between 4.6 and 92 mg/L. DOC of the filtered stock solution was 550 mg/L, corresponding to 919 mg test item/L.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
no data

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h
Post exposure observation period:
none

Test conditions

Hardness:
no data
Test temperature:
no data
pH:
no data
Dissolved oxygen:
no data
Salinity:
no data
Nominal and measured concentrations:
no data
Details on test conditions:
TEST SYSTEM
according to DIN 38412, part 11

TEST MEDIUM / WATER PARAMETERS
according to DIN 38412, part 11

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
according to DIN 38412, part 11

TEST CONCENTRATIONS
test concentrations between 4.6 and 92 mg/L
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
< 4.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
11.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 8.7 - 16.0 mg/L
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
92 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
no data
Results with reference substance (positive control):
no data
Reported statistics and error estimates:
no data

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 24-h EC50 of Daphnia magna for 4-chlorbenzaldehyde was determined to be 11.8 mg/L.