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EC number: 228-291-1 | CAS number: 6219-71-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Acute Oral Toxicity:
In Acute oral toxicity ,LD50 value was predicted based on OECD QSAR toolbox for target substance 2-chlorobenzene-1,4-diammonium sulphate (6219-71-2) was estimated to be 3976.64 mg/kg bw,and for differentstudies available on the structurally similar read across substance 6-aminonaphthalene-2-sulphonic acid (93-00-5) was considered to be 5000 mg/kg bw and for 1,3-Phenylenediamine-4-sulfonic acid (88-63-1) was considered to be 3480 mg/kg bw. All these studies concluded that the LD50 value is greater than 2000 mg/kg bw. Thus, comparing this value with the criteria of CLP regulation, 2-chlorobenzene-1,4-diammonium sulphate (6219-71-2) cannot be classified for acute oral toxicity.
Acute Inhalation Toxicity:
2-chlorobenzene-1,4-diammonium sulphate has very low vapor pressure (2.12E-012 Pa), so the potential for the generation of inhalable vapours is very low. Also the normal conditions of use of this substance will not result in aerosols, particles or droplets of an inhalable size, so exposure to humans via the inhalatory route will be highly unlikely and therefore this end point for acute inhalation toxicity was considered for waiver.
Acute Dermal Toxicity:
In Acute dermal toxicity, LD50 value was predicted based on OECD QSAR toolbox for target substance 2-chlorobenzene-1,4-diammonium sulphate (6219-71-2) was estimated to be 20718.78 mg/kg bw andfor differentstudies available on structurally similar read across substance 6-aminonaphthalene-2-sulphonic acid (93-00-5)was considered to be >2000 mg/kg bw and for functionally similar read across substance 4,4'-Diaminodiphenyl sulfone (80-08-0) was considered to be >4000 mg/kg bw.All these studies concluded that the LD50 value is greater than 2000 mg/kg bw. Thus, comparing this value with the criteria of CLP regulation, 2-chlorobenzene-1,4-diammonium sulphate (6219-71-2) cannot be classified for acute dermal toxicity.
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- (Q)SAR
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- results derived from a valid (Q)SAR model and falling into its applicability domain, with limited documentation / justification
- Justification for type of information:
- Data is predicted using OECD QSAR toolbox version 3.3 and QMRF report has been attached
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- Prediction was done by using OECD QSAR toolbox v3.3,2017
- GLP compliance:
- not specified
- Test type:
- other: estimated data
- Limit test:
- no
- Specific details on test material used for the study:
- IUPAC name: 2-chlorobenzene-1,4-diammonium sulphate
Molecular formula: C6H7ClN2.xH2O4S
Molecular weight:240.6661
substance type: organic
physical state: light grey colored solid powder.
InChI:1S/C6H7ClN2.H2O4S/c7-5-3-4(8)1-2-6(5)9;1-5(2,3)4/h1-3H,8-9H2;(H2,1,2,3,4)
Smiles:S(=O)(=O)(O)O.c1(c(ccc(c1)N)N)Cl - Species:
- rat
- Strain:
- other: Gassner
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- No data available
- Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Details on oral exposure:
- No data available
- Doses:
- 3976.64 mg/kg bw
- No. of animals per sex per dose:
- No data available
- Control animals:
- not specified
- Details on study design:
- No data available
- Statistics:
- No data available
- Preliminary study:
- No data available
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 3 976.64 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: 50% mortality was observed
- Mortality:
- No data available
- Clinical signs:
- other: No data available
- Gross pathology:
- No data available
- Other findings:
- No data available
- Interpretation of results:
- other: not classified
- Conclusions:
- The LD50 was estimated to be 3976.64 mg/kg bw,when male and female Gassner rats were orally exposed with 2-chlorobenzene-1,4-diammonium sulphate (6219-71-2) via gavage.
- Executive summary:
In a prediction done by SSS (2017) using the OECD QSAR toolbox with log kow as the primary descriptor, the acute oral toxicity was estimated for 2-chlorobenzene-1,4-diammonium sulphate(6219-71-2) .The LD50 was estimated to be 3976.64 mg/kg bw,when male and female Gassner rats were orally exposed with 2-chlorobenzene-1,4-diammonium sulphate(6219-71-2) via gavage.
Reference
The
prediction was based on dataset comprised from the following
descriptors: LD50
Estimation method: Takes average value from the 5 nearest neighbours
Domain logical expression:Result: In Domain
(((((((("a"
or "b" or "c" or "d" or "e" )
and "f" )
and "g" )
and ("h"
and (
not "i")
)
)
and "j" )
and ("k"
and (
not "l")
)
)
and ("m"
and (
not "n")
)
)
and ("o"
and "p" )
)
Domain
logical expression index: "a"
Referential
boundary: The
target chemical should be classified as Anilines (Acute toxicity) by
US-EPA New Chemical Categories
Domain
logical expression index: "b"
Referential
boundary: The
target chemical should be classified as Radical OR Radical >> Radical
mechanism via ROS formation (indirect) OR Radical >> Radical mechanism
via ROS formation (indirect) >> Single-Ring Substituted Primary Aromatic
Amines OR SN1 OR SN1 >> Nucleophilic attack after metabolic nitrenium
ion formation OR SN1 >> Nucleophilic attack after metabolic nitrenium
ion formation >> Single-Ring Substituted Primary Aromatic Amines by DNA
binding by OASIS v.1.3 ONLY
Domain
logical expression index: "c"
Referential
boundary: The
target chemical should be classified as SN1 OR SN1 >> Nitrenium Ion
formation OR SN1 >> Nitrenium Ion formation >> Primary aromatic amine by
DNA binding by OECD ONLY
Domain
logical expression index: "d"
Referential
boundary: The
target chemical should be classified as Weak binder, NH2 group by
Estrogen Receptor Binding
Domain
logical expression index: "e"
Referential
boundary: The
target chemical should be classified as Anilines (amino-para) OR
Anilines (Unhindered) OR Inorganic Compound by Aquatic toxicity
classification by ECOSAR ONLY
Domain
logical expression index: "f"
Referential
boundary: The
target chemical should be classified as No alert found AND Radical AND
Radical >> Radical mechanism via ROS formation (indirect) AND Radical >>
Radical mechanism via ROS formation (indirect) >> Single-Ring
Substituted Primary Aromatic Amines AND SN1 AND SN1 >> Nucleophilic
attack after metabolic nitrenium ion formation AND SN1 >> Nucleophilic
attack after metabolic nitrenium ion formation >> Single-Ring
Substituted Primary Aromatic Amines by DNA binding by OASIS v.1.3 ONLY
Domain
logical expression index: "g"
Referential
boundary: The
target chemical should be classified as No alert found AND SN1 AND SN1
>> Nitrenium Ion formation AND SN1 >> Nitrenium Ion formation >> Primary
aromatic amine by DNA binding by OECD ONLY
Domain
logical expression index: "h"
Referential
boundary: The
target chemical should be classified as Non binder, non cyclic structure
AND Weak binder, NH2 group by Estrogen Receptor Binding
Domain
logical expression index: "i"
Referential
boundary: The
target chemical should be classified as Moderate binder, NH2 group OR
Non binder, impaired OH or NH2 group OR Non binder, MW>500 OR Non
binder, without OH or NH2 group OR Strong binder, NH2 group OR Very
strong binder, OH group OR Weak binder, OH group by Estrogen Receptor
Binding
Domain
logical expression index: "j"
Referential
boundary: The
target chemical should be classified as Reactive unspecified by Acute
aquatic toxicity MOA by OASIS ONLY
Domain
logical expression index: "k"
Referential
boundary: The
target chemical should be classified as Group 14 - Carbon C AND Group 15
- Nitrogen N AND Group 16 - Oxygen O AND Group 16 - Sulfur S AND Group
17 - Halogens Cl AND Group 17 - Halogens F,Cl,Br,I,At by Chemical
elements
Domain
logical expression index: "l"
Referential
boundary: The
target chemical should be classified as Group 15 - Phosphorus P OR Group
17 - Halogens Br OR Group 17 - Halogens F by Chemical elements
Domain
logical expression index: "m"
Referential
boundary: The
target chemical should be classified as Aminoaniline, para AND Aniline
AND Aryl AND Aryl halide AND No functional group found AND Precursors
quinoid compounds by Organic Functional groups
Domain
logical expression index: "n"
Referential
boundary: The
target chemical should be classified as Alkyl arenes OR Amidine OR
Aminoaniline, ortho OR Benzimidazole OR Carboxylic acid OR Fused
carbocyclic aromatic OR Unsaturated heterocyclic amine OR Unsaturated
heterocyclic fragment by Organic Functional groups
Domain
logical expression index: "o"
Parametric
boundary:The
target chemical should have a value of log Kow which is >= -2.2
Domain
logical expression index: "p"
Parametric
boundary:The
target chemical should have a value of log Kow which is <= 0.483
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 3 976.64 mg/kg bw
- Quality of whole database:
- Data is Klimisch 2 and from QSAR toolbox 3.3
Acute toxicity: via inhalation route
Link to relevant study records
- Endpoint:
- acute toxicity: inhalation
- Data waiving:
- other justification
- Justification for data waiving:
- other:
Reference
Endpoint conclusion
- Quality of whole database:
- waiver
Acute toxicity: via dermal route
Link to relevant study records
- Endpoint:
- acute toxicity: dermal
- Type of information:
- (Q)SAR
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- results derived from a valid (Q)SAR model and falling into its applicability domain, with limited documentation / justification
- Justification for type of information:
- Data is predicted using OECD QSAR toolbox version 3.3 and QMRF report has been attached
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- Prediction was done by using OECD QSAR toolbox v3.3,2017
- GLP compliance:
- not specified
- Test type:
- other: estimated data
- Limit test:
- no
- Specific details on test material used for the study:
- IUPAC name: 2-chlorobenzene-1,4-diammonium sulphate
Molecular formula: C6H7ClN2.xH2O4S
Molecular weight:240.6661
substance type: organic
physical state: light grey colored solid powder.
InChI:1S/C6H7ClN2.H2O4S/c7-5-3-4(8)1-2-6(5)9;1-5(2,3)4/h1-3H,8-9H2;(H2,1,2,3,4)
Smiles:S(=O)(=O)(O)O.c1(c(ccc(c1)N)N)Cl - Species:
- rabbit
- Strain:
- New Zealand White
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- No data available
- Type of coverage:
- semiocclusive
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- No data available
- Duration of exposure:
- 24 hours
- Doses:
- 20718.78 mg/kg bw
- No. of animals per sex per dose:
- No data available
- Control animals:
- not specified
- Details on study design:
- No data available
- Statistics:
- No data available
- Preliminary study:
- No data available
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 20 718.78 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: 50% Mortality was observed
- Mortality:
- No data available
- Clinical signs:
- other: No data available
- Gross pathology:
- No data available
- Other findings:
- No data available
- Interpretation of results:
- other: not classified
- Conclusions:
- The LD50 value was estimated to be 20718.78 mg/kg bw,when male and female New Zealand White rabbits were semiocclusively exposed with 2-chlorobenzene-1,4-diammonium sulphate (6219-71-2) by dermal application for 24 hours.
- Executive summary:
In a prediction done by SSS (2017) using the OECD QSAR toolbox with log kow as the primary descriptor, the acute dermal toxicity was estimated for 2-chlorobenzene-1,4-diammonium sulphate(6219-71-2).The LD50 was estimated to be 20718.78 mg/kg bw,when male and female New Zealand White rabbits were semiocclusively exposed with 2-chlorobenzene-1,4-diammonium sulphate(6219-71-2)by dermal application for 24 hours.
Reference
The
prediction was based on dataset comprised from the following
descriptors: LD50
Estimation method: Takes average value from the 6 nearest neighbours
Domain logical expression:Result: In Domain
((((((((("a"
or "b" or "c" or "d" or "e" )
and "f" )
and ("g"
and (
not "h")
)
)
and ("i"
and (
not "j")
)
)
and ("k"
and (
not "l")
)
)
and ("m"
and (
not "n")
)
)
and ("o"
and (
not "p")
)
)
and "q" )
and ("r"
and "s" )
)
Domain
logical expression index: "a"
Referential
boundary: The
target chemical should be classified as Anilines (Acute toxicity) by
US-EPA New Chemical Categories
Domain
logical expression index: "b"
Referential
boundary: The
target chemical should be classified as Radical OR Radical >> Radical
mechanism via ROS formation (indirect) OR Radical >> Radical mechanism
via ROS formation (indirect) >> Single-Ring Substituted Primary Aromatic
Amines OR SN1 OR SN1 >> Nucleophilic attack after metabolic nitrenium
ion formation OR SN1 >> Nucleophilic attack after metabolic nitrenium
ion formation >> Single-Ring Substituted Primary Aromatic Amines by DNA
binding by OASIS v.1.3 ONLY
Domain
logical expression index: "c"
Referential
boundary: The
target chemical should be classified as SN1 OR SN1 >> Nitrenium Ion
formation OR SN1 >> Nitrenium Ion formation >> Primary aromatic amine by
DNA binding by OECD ONLY
Domain
logical expression index: "d"
Referential
boundary: The
target chemical should be classified as Weak binder, NH2 group by
Estrogen Receptor Binding
Domain
logical expression index: "e"
Referential
boundary: The
target chemical should be classified as Anilines (amino-para) OR
Anilines (Unhindered) OR Inorganic Compound by Aquatic toxicity
classification by ECOSAR ONLY
Domain
logical expression index: "f"
Referential
boundary: The
target chemical should be classified as High (Class III) AND Low (Class
I) by Toxic hazard classification by Cramer (with extensions) ONLY
Domain
logical expression index: "g"
Referential
boundary: The
target chemical should be classified as Aromatic amines AND Inclusion
rules not met by Skin irritation/corrosion Inclusion rules by BfR
Domain
logical expression index: "h"
Referential
boundary: The
target chemical should be classified as Primary and secondary aliphatic
amines by Skin irritation/corrosion Inclusion rules by BfR
Domain
logical expression index: "i"
Referential
boundary: The
target chemical should be classified as (!Undefined)Group All Lipid
Solubility < 0.01 g/kg AND (!Undefined)Group CNHal Lipid Solubility <
400 g/kg AND Exclusion rules not met by Eye irritation/corrosion
Exclusion rules by BfR
Domain
logical expression index: "j"
Referential
boundary: The
target chemical should be classified as Group All Melting Point > 200 C
OR Group CNHal Aqueous Solubility < 0.004 g/L OR Group CNHal Aqueous
Solubility < 0.1 g/L OR Group CNHal log Kow > 3.8 OR Group CNS log Kow >
1.5 OR Group CNS Melting Point > 50 C by Eye irritation/corrosion
Exclusion rules by BfR
Domain
logical expression index: "k"
Referential
boundary: The
target chemical should be classified as Group 14 - Carbon C AND Group 15
- Nitrogen N AND Group 16 - Oxygen O AND Group 16 - Sulfur S AND Group
17 - Halogens Cl AND Group 17 - Halogens F,Cl,Br,I,At by Chemical
elements
Domain
logical expression index: "l"
Referential
boundary: The
target chemical should be classified as Group 15 - Phosphorus P OR Group
17 - Halogens I by Chemical elements
Domain
logical expression index: "m"
Referential
boundary: The
target chemical should be classified as Aminoaniline, para AND Aniline
AND Aryl AND Aryl halide AND No functional group found AND Precursors
quinoid compounds by Organic Functional groups
Domain
logical expression index: "n"
Referential
boundary: The
target chemical should be classified as Aminoaniline, ortho by Organic
Functional groups
Domain
logical expression index: "o"
Referential
boundary: The
target chemical should be classified as Aryl halide AND No functional
group found AND Overlapping groups AND Precursors quinoid compounds by
Organic Functional groups (nested)
Domain
logical expression index: "p"
Referential
boundary: The
target chemical should be classified as Alkyl arenes OR Aniline OR
Nitrobenzene by Organic Functional groups (nested)
Domain
logical expression index: "q"
Similarity
boundary:Target:
Nc1ccc(N)cc1Cl_OS(O)(=O)=O
Threshold=20%,
Dice(Atom centered fragments)
Atom type; Count H attached; Hybridization
Domain
logical expression index: "r"
Parametric
boundary:The
target chemical should have a value of log Kow which is >= -1.23
Domain
logical expression index: "s"
Parametric
boundary:The
target chemical should have a value of log Kow which is <= 0.241
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 20 718.78 mg/kg bw
- Quality of whole database:
- Data is Klimisch 2 and from QSAR toolbox 3.3
Additional information
Acute Oral Toxicity:
In different studies, 2-chlorobenzene-1,4-diammonium sulphate (6219-71-2) has been investigated for acute oral toxicity to a greater or lesser extent. Often the studies are based on in vivo experiments and estimated data in rodents, i.e. most commonly in rats for 2-chlorobenzene-1,4-diammonium sulphate (6219-71-2) along with the study available on the structurally similar read across substance 6-aminonaphthalene-2-sulphonic acid (93-00-5) and 1,3-Phenylenediamine-4-sulfonic acid (88-63-1). The predicted data using the OECD QSAR toolbox has also been compared with the experimental studies. The studies are summarized as below –
In a prediction done by SSS (2017) using the OECD QSAR toolbox with log kow as the primary descriptor, the acute oral toxicity was estimated for 2-chlorobenzene-1,4-diammonium sulphate(6219-71-2).The LD50 was estimated to be 3976.64 mg/kg bw,when male and female Gassner rats were orally exposed with 2-chlorobenzene-1,4-diammonium sulphate(6219-71-2) via gavage.
The above experimental study was supported by Sustainability Support Services (Europe) AB (study number: 19166, 2017-08-10) for the structurally similar read across substance 6-aminonaphthalene-2-sulphonic acid (93-00-5). The acute oral toxicity profile of 6-aminonaphthalene-2-sulphonic acid (CAS No. 93-00-5)in Sprague Dawley rats. Initially, three female animals were treated at the dose level of 300 mg/kg body weight of the test item (Step - I). Administration of the test item at 300 mg/kg did not result in any signs of toxicity and mortality at 24 hours after the dosing. As no mortality was observed at 24 hours after the dosing, three female animals were added to the study and treated with the same dose of 300 mg/kg of the test item (Step - II). Administration of the test item at 300 mg/kg did not result in any signs of toxicity and mortality after the dosing. No mortality was observed at 300 mg/kg dose group, hence additional three female animals were treated with the higher dose of 2000 mg/kg of the test item (Step - I). Administration of the test item at 2000 mg/kg did not result in any signs of toxicity and mortality after the dosing. As no mortality were observed at 24 hours after the dosing, additional three female animals were treated with the higher dose of 2000 mg/kg of the test item (Step - II). Administration of the test item at 2000 mg/kg did not result in any signs of toxicity and mortality after the dosing.Gross pathological examination did not reveal any abnormalities in animals from 300 mg/kg and 2000 mg/kg dose groups. The acute oral LD50 (Cut-off value) of 6-aminonaphthalene-2-sulphonic acid (CAS No. 93-00-5) was 5000 mg/kg body weight. Thus, it was concluded that the acute toxicity study of 6-aminonaphthalene-2-sulphonic acid (CAS No. 93-00-5), when administered via oral route in Sprague Dawley rats falls into the “Category Unclassified” criteria of CLP.
This is further supported by Richard J Lewis (Sax’s Dangerous Properties of Industrial Materials, 12th Edition); IFA GESTIS (Gestis Substance Database,2017) and U.S. National Library of Medicine (Chemidplus Database,U.S. National Library of Medicine,2017) for the structurally similar read across substance 1,3-Phenylenediamine-4-sulfonic acid (88-63-1).Acute oral toxicity test of 1,3-Phenylenediamine-4-sulfonic acid was conducted with rat in the concentration of 3480 mg/kg bw orally. 50% mortality was observed in treated rat. Therefore, LD50 was considered to be 3480 mg/kg, when rats were treated with 2,4-diaminobenzenesulphonic acid orally.
Thus, based on the above studies on 2-chlorobenzene-1,4-diammonium sulphate(6219-71-2) and it’s structurally similar read across substances, it can be concluded that LD50 value is greater than 2000 mg/kg bw. Thus, comparing this value with the criteria of CLP regulation, 2-chlorobenzene-1,4-diammonium sulphate(6219-71-2) cannot be classified for acute oral toxicity.
Acute Inhalation Toxicity:
2-chlorobenzene-1,4-diammonium sulphate has very low vapor pressure (2.12E-012 Pa), so the potential for the generation of inhalable vapours is very low. Also the normal conditions of use of this substance will not result in aerosols, particles or droplets of an inhalable size, so exposure to humans via the inhalatory route will be highly unlikely and therefore this end point for acute inhalation toxicity was considered for waiver.
Acute Dermal Toxicity:
In different studies, 2-chlorobenzene-1,4-diammonium sulphate (6219-71-2) has been investigated for acute dermal toxicity to a greater or lesser extent. Often the studies are based on in vivo experiments and estimated data in rodents, i.e. most commonly in rabbits for 2-chlorobenzene-1,4-diammonium sulphate (6219-71-2) along with the study available on structurally similar read across substance 6-aminonaphthalene-2-sulphonic acid (93-00-5)and on functionally similar read across substance 4,4'-Diaminodiphenyl sulfone (80-08-0). The predicted data using the OECD QSAR toolbox has also been compared with the experimental studies. The studies are summarized as below –
In a prediction done by SSS (2017) using the OECD QSAR toolbox with log kow as the primary descriptor, the acute dermal toxicity was estimated for 2-chlorobenzene-1,4-diammonium sulphate(6219-71-2).The LD50 was estimated to be 20718.78 mg/kg bw,when male and female New Zealand White rabbits were semiocclusively exposed with 2-chlorobenzene-1,4-diammonium sulphate(6219-71-2)by dermal application for 24 hours.
The above study was further supported by Sustainability Support Services (Europe) AB (study number: 19167, 2017-08-10) for the structurally similar read across substance 6-aminonaphthalene-2-sulphonic acid (93-00-5).The acute dermal toxicity profile of 6-aminonaphthalene-2-sulphonic acid (CAS No. 93-00-5)in Sprague Dawley rats.The test item was applied to shorn skin of 5 male and 5 female animals at 2000 mg/kg body weight. Administration of the test item at 2000 mg/kg did not result in any skin reaction at the site of application during the study period of 14 days. Administration of the test item did not result in any signs of toxicity and mortality during the study period of 14 days.Animals exhibited normal body weight gain through the study period of 14 days.Gross pathological examination did not reveal any abnormalities attributable to the treatment. It was concluded that the acute dermal median lethal dose (LD50) of 6-aminonaphthalene-2-sulphonic acid (93-00-5), when administered to male and female Sprague Dawley rats was considered to be >2000 mg/kg body weight.Thus, according to CLP criteria for acute toxicity rating for the chemicals, it infers that 6-aminonaphthalene-2-sulphonic acid (CAS No. 93-00-5) does not classify as an acute dermal toxicant.
Also these results are further supported by CHARLES P. CARPENTER et. al. (TOXICOLOGY AND APPLIED PHARMACOLOGY 28,313-319 (1974)); U.S. National Library of Medicine (Chemidplus Database,U.S. National Library of Medicine,2017) and IFA GESTIS (Gestis Substance Database,2017) for the functionally similar read substance4,4'-Diaminodiphenyl sulfone (80-08-0).In acute dermal toxicity study,rabbits were treated with 4,4'-Diaminodiphenyl sulfone (80-08-0)in the concentration of 4000 mg/kg bw by dermal application.No mortality was observed in treated rabbits at dose 4000 mg/kg bw.Therefore, LD50 value was considered to be >4000 mg/kg bw,when rabbits were treated with 4,4'-Diaminodiphenyl sulfone (80-08-0) by dermal application.
Thus, based on the above studies on 2-chlorobenzene-1,4-diammonium sulphate (6219-71-2) and it’s structurally and functionally similar read across substances, it can be concluded that LD50 value is greater than 2000 mg/kg bw. Thus, comparing this value with the criteria of CLP regulation, 2-chlorobenzene-1,4-diammonium sulphate (6219-71-2) cannot be classified for acute dermal toxicity.
Justification for classification or non-classification
Based on the above studies and prediction on 2-chlorobenzene-1,4-diammonium sulphate (6219-71-2) and it’s structurally and functionally similar read across substances, it can be concluded that LD50 value is greater than 2000 mg/kg bw. Thus, comparing this value with the criteria of CLP regulation, 2-chlorobenzene-1,4-diammonium sulphate (6219-71-2) cannot be classified for acute oral and dermal toxicity. For Acute inhalation toxicity wavier was added so, not possible to classify.
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