Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report Date:
2017

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test solution was prepared by using a stock solution which was prepared by mixing the test sample and medium, and stirring them until being dissolved.
pH adjusted; Test solution was prepared by using a stock solution prepared by adjusting pH around medium.
- Controls: medium without test item

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Common name: Pseudokirchneriella suhcapitata
- Source: American Type Culture Collection; Strain number: ATCC 22662
- Method of cultivation:

Study design

Test type:
other: rotary shaking
Water media type:
freshwater
Total exposure duration:
72 h

Test conditions

Test temperature:
21-24°C (±2°C)
pH:
pH of test solutions (pH unadjusted): 7.9 - 9.8
pH of test solutions (pH adjusted): start - 7.9
end - 8.7 - 10.0
Nominal and measured concentrations:
1, 3.16, 10, 31.6 and 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Sterilized 500 mL Erlenmeyer flask
- Type: closed
- Initial cells density: 0.5 x 104 cells/mL
- Volume of test solution: 600 mI/control level (100 mI/test vessel); 300 mI/exposure level (100 mI/test vessel)
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: with and without
- Light intensity and quality: Nominal 90 µmoI·m-2·s-1 ± 20% (within±15% from the average light intensity). Continuous illumination provided by fluorescent lights with wavelength range of 400-700nm.

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Remarks:
pH adjusted
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Remarks:
pH unadjusted
Effect conc.:
18.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Remarks:
pH adjusted
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Remarks:
pH unadjusted
Effect conc.:
1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
ErC50 of the test item based on the growth rate was > 100 mg/L.
NOEC based on growth rate was determined to be >= 100 mg/L.