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Short-term toxicity to aquatic invertebrates

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to aquatic invertebrate was predicted trisodium 2-{[3-methyl-5-oxo-1-(4-{[2-(sulfonatooxy)ethyl]sulfonyl}phenyl)-4,5-dihydro-1H-pyrazol-4-...(CAS: 20298-05-9). EC50 intoxication value was estimated to be 658.105 mg/l for Daphnia magna for 48 hrs duration. It was concluded that trisodium 2-{[3-methyl-5-oxo-1-(4-{[2-(sulfonatooxy)ethyl]sulfonyl}phenyl)-4,5-dihydro-1H-pyrazol-4-...(CAS: 20298-05-9) was likely to be not toxic to aquatic invertebrate.

Toxicity to aquatic algae and cyanobacteria

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to aquatic algae was predicted trisodium 2-{[3-methyl-5-oxo-1-(4-{[2-(sulfonatooxy)ethyl]sulfonyl}phenyl)-4,5-dihydro-1H-pyrazol-4-...(CAS: 20298-05-9). EC50 intoxication value was estimated to be 169.602 mg/l for Desmodesmus subspicatus for 72 hrs duration. It was concluded that trisodium 2-{[3-methyl-5-oxo-1-(4-{[2-(sulfonatooxy)ethyl]sulfonyl}phenyl)-4,5-dihydro-1H-pyrazol-4-...(CAS: 20298-05-9) was likely to be not toxic to aquatic algae.

Additional information

Short-term toxicity to aquatic invertebrates

Following three studies of target chemical and structurally similar read across includes predicted data and experimental data to conclude the toxicity extent of trisodium 2-{[3-methyl-5-oxo-1-(4-{[2-(sulfonatooxy)ethyl]sulfonyl}phenyl)-4,5-dihydro-1H-pyrazol-4-...(CAS: 20298-05-9) towards aquatic invertebrate is summarized as follows:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to aquatic invertebrate was predicted trisodium 2-{[3-methyl-5-oxo-1-(4-{[2-(sulfonatooxy)ethyl]sulfonyl}phenyl)-4,5-dihydro-1H-pyrazol-4-...(CAS: 20298-05-9). EC50 intoxication value was estimated to be 658.105 mg/l for Daphnia magna for 48 hrs duration. It was concluded that trisodium 2-{[3-methyl-5-oxo-1-(4-{[2-(sulfonatooxy)ethyl]sulfonyl}phenyl)-4,5-dihydro-1H-pyrazol-4-...(CAS: 20298-05-9) was likely to be not toxic to aquatic invertebrate.

The above predicted data of target chemical is supported by the experimental study of structurally similar read across

disodium 6-hydroxy-5-[(4-sulfonatophenyl)diazenyl]naphthalene-2-sulfonatE(CAS: 2783 -94 -0) from the ABITEC Lab report 2016, suggests that theDetermination of the inhibition of the mobility of daphnids was carried out with the substancedisodium 6-hydroxy-5-[(4-sulfonatophenyl)diazenyl]naphthalene-2-sulfonateaccording to OECD Guideline 202.

The test substance was tested at the concentration 100 mg/L. 0% Daphnia were immobilised at the end of the test.

The median effective concentration (EC50) for the test substance,disodium 6-hydroxy-5-[(4-sulfonatophenyl)diazenyl]naphthalene-2-sulfonate, in Daphnia magna was determined to be > 100 mg/L for immobilisation effects.

This value indicates that the substance is not likely to be hazardous to aquatic invertebrates as per the CLP criteria.

Another supporting experimental study for the structurally similar read across ε-caprolactam (CAS: 105-60-2) from the publication Chemosphere 1997, also suggests that the Short term toxicity to aquatic invertebrate test was carried for ε-caprolactam to study the effects on aquatic invertebrate. The Effective concentration to 50% of Daphnia magna when exposed to ε-caprolactam for 48 h is 2430 mg/L . It can be concluded from the Intoxication value that the ε-caprolactam is not toxic to the aquatic invertebrate and can be considered as “not classified” as per the classification criteria for aquatic environment.

 

Thus based on the effect concentrations which is in the range >100 mg/l to 2430 mg/l give the conclusion that test substance trisodium 2-{[3-methyl-5-oxo-1-(4-{[2-(sulfonatooxy)ethyl]sulfonyl}phenyl)-4,5-dihydro-1H-pyrazol-4-...(CAS: 20298-05-9) was likely to be not toxic to aquatic invertebrate at environmentally relevant concentrations and can be considered to be “not classified” as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria

Following three studies of target chemical and structurally similar read across includes predicted data and experimental data to conclude the toxicity extent of trisodium 2-{[3-methyl-5-oxo-1-(4-{[2-(sulfonatooxy)ethyl]sulfonyl}phenyl)-4,5-dihydro-1H-pyrazol-4-...(CAS: 20298-05-9) towards aquatic algae is summarized as follows:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to aquatic algae was predicted trisodium 2-{[3-methyl-5-oxo-1-(4-{[2-(sulfonatooxy)ethyl]sulfonyl}phenyl)-4,5-dihydro-1H-pyrazol-4-...(CAS: 20298-05-9). EC50 intoxication value was estimated to be 169.602 mg/l for Desmodesmus subspicatus for 72 hrs duration. It was concluded that trisodium 2-{[3-methyl-5-oxo-1-(4-{[2-(sulfonatooxy)ethyl]sulfonyl}phenyl)-4,5-dihydro-1H-pyrazol-4-...(CAS: 20298-05-9) was likely to be not toxic to aquatic algae.

The above predicted data of target chemical is supported by the experimental study of structurally similar read across

disodium 6-hydroxy-5-[(4-sulfonatophenyl)diazenyl]naphthalene-2-sulfonatE(CAS: 2783 -94 -0) from the ABITEC Lab report 2016, suggests that the

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substancedisodium 6-hydroxy-5-[(4-sulfonatophenyl)diazenyl]naphthalene-2-sulfonateaccording to OECD Guideline 201.

The test substance was dissolved in DMSO and tested at the concentrations 0, 17, 30, 55, 100 and 185 mg/L. Effects on the growth rate of the organism were studied.

The median effective concentration (EC50) for the test substance,disodium 6-hydroxy-5-[(4-sulfonatophenyl)diazenyl]naphthalene-2-sulfonate, in Desmodesmus subspicatus was determined to be 113.2 mg/L.

This value indicates that the substance is not likely to be hazardous to aquatic algae as per the CLP criteria.

Another supporting experimental study for the structurally similar read across ε-caprolactam (CAS: 105-60-2) from the publication Chemosphere 1997, also suggests that theShort term toxicity to aquatic invertebrate test was carried for ε-caprolactam to study the effects on aquatic invertebrate. decreasing trend ogf Population (Abundance) was measured during the test.

Effective concentration to 50% of Pseudokirchneriella subcapitata when exposed to ε-caprolactam for 72 h is 4550 mg/L. It can be concluded from the Population (Abundance) value that the  ε-caprolactam is not toxic to the aquatic algae and can be considered as “not classified” as per the classification criteria for aquatic environment.

Thus based on the effect concentrations which is in the range 113.2 mg/l to 4550 mg/l give the conclusion that test substance trisodium 2-{[3-methyl-5-oxo-1-(4-{[2-(sulfonatooxy)ethyl]sulfonyl}phenyl)-4,5-dihydro-1H-pyrazol-4-...(CAS: 20298-05-9) was likely to be not toxic to aquatic algae at environmentally relevant concentrations and can be considered to be “not classified” as per the CLP classification criteria.