Dimethyl[2-[(2-methyl-1-oxoallyl)oxy]ethyl](3-sulphopropyl)ammonium hydroxide

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2016-04-25 - 2016-06-28

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Version / remarks:

OECD Guidelines for the Testing of Chemicals Part 301 F, adopted 17. Jul. 1992 „Manometric Respirometry Test“

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Version / remarks:

Council Regulation (EC) No. 440/2008, Guideline C.4-D, adopted 30. May 2008 “Manometric Respirometry Test”

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

by the Landesamt für Umwelt, Wasserwirtschaft und Gewerbeaufsicht, Kaiser-Friedrich-Straße 7, 55116 Mainz

Test material

Test material information

Constituent 1

Reference substance name:

Dimethyl[2-[(2-methyl-1-oxoallyl)oxy]ethyl](3-sulphopropyl)ammonium hydroxide

EC Number:

222-860-8

EC Name:

Dimethyl[2-[(2-methyl-1-oxoallyl)oxy]ethyl](3-sulphopropyl)ammonium hydroxide

Cas Number:

3637-26-1

Molecular formula:

C11H21NO5S

IUPAC Name:

dimethyl[2-[(2-methyl-1-oxoallyl)oxy]ethyl](3-sulphopropyl)ammonium hydroxide

Test material form:

solid: particulate/powder

Specific details on test material used for the study:

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: The test item was stored in a closed vessel at 17.0 – 23.3 °C, away from humidity.

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge from a biologic sewage treatment plant was used. The chosen plant is treating mostly household sewage.
The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant, Im Altenschemel, NW-Lachen-Speyerdorf.
- Preparation of inoculum for exposure: The sludge was filtrated, washed with tap water (2x), then washed with and re-suspended in test medium. It was then aerated.
- Concentration of sludge: The dry matter was determined as 4960 mg suspended solids/L.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

53 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

100 mg/L

Based on:

ThOD

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium:
Stock solutions
a) 8.5 g KH2PO4, 21.75 g K2HPO4, 33.4 g Na2HPO4 x 2 H2O, 0.5 g NH4Cl and 1000 mL H2O demin. ad
b) 36.4 g CaCl2 x 2H2O and 1000 mL H2O demin. ad
c) 22.5 g MgSO4 x 7H2O and 1000 mL H2O demin. ad
d) 0.25 g FeCl3 x 6H2O, 0.4 g Na2EDTA x 2H2O and 1000 mL H2O demin. ad
Test Medium
10 mL solution a
1 mL solution b
1 mL solution c
1 mL solution d
1000 mL H2O demin. ad
- Test temperature: 20.5 – 23.7 °C
- pH adjusted: yes, the pH was measured with 7.6, the test abiotic test vessel was adjusted to 7.3.
- Aeration of dilution water: not stated
- Suspended solids concentration: 25 mg/l

TEST SYSTEM
- Culturing apparatus: 500 mL-flasks were used as test vessels.
All glassware was cleaned with the laboratory cleaning agent and then rinsed with tap water (thrice), diluted HCL (once), tap water (thrice) and deionised water (thrice).
- Number of culture flasks/concentration:
Number of controls: 3 (medium and inoculum)
Number of positive control vessels: 3 (sodium-benzoate, medium and inoculum)
Number of test vessels: 3 (test item, medium and inoculum)
Abiotic control: 1 (test item and 10 mL 1% HgCl2 solution/L)
Toxicity control: 1 (100 mg ThOD each of test item and positive control)
- Measuring equipment: The vessels were connected to the OxiTop® measuring heads and they were started via the OxiTop® Controllers.
- Other:
Volume of test vessels: 500 mL
Volume test solution: 250 mL (toxicity control flask 125 mL)

CONTROL AND BLANK SYSTEM
- Inoculum blank: 3 (medium and inoculum)
- Abiotic sterile control: 1 (test item and 10 mL 1% HgCl2 solution/L)
- Toxicity control: 1 (100 mg ThOD each of test item and positive control)

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

Preliminary study:

No information about a preliminary study are available.

Test performance:

No unusual observations during test period observed.

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

8

Sampling time:

28 d

Remarks on result:

other:

Remarks:

The 10-day-window was not detected.

BOD5 / COD results

Results with reference substance:

Degradation after 3 days > 60% (validity criterion fulfilled)
Positive control 1: 88.1 % degradation after 28 days
Positive control 2: 84.5 % degradation after 28 days
Positive control : 79.1 % degradation after 28 days

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The study was performed according to OECD TG301F and EU Method C.4-D (GLP) without deviations and therefore considered to be of the highest quality (reliability Klimisch 1). The validity criteria of the test system are fulfilled. Within 28 days, a degradation of 8% was determined for the test substance; the 10 day window criterion was not passed. Therefore, the test substance is considered to be not readily biodegradable.

Executive summary:

The study was conducted following OECD 301F / EU C.4-D under GLP on N,N-Dimethyl-N-(2-methacryloyloxyethyl)-N-(3-sulfopropyl)-ammonium betaine; (SPE)

Two experiments were performed. In the first experiment, the measuring heads showed leakages and therefore inconsistent data. Therefore, the experiment was repeated under the same conditions. The results of the first experiment are not stated in this report, but will be kept together with the other raw data in the GLP archive of the test facility.

The test item N,N-Dimethyl-N-(2-methacryloyloxyethyl)-N-(3-sulfopropyl)-ammonium betaine; (SPE) was tested using a concentration of 53 mg test item/L (equivalent to a theoretical oxygen demand of 100.15 mg O2/L). Activated sludge was used as inoculum. The test was left running for 28 days.

All validity criteria were met. The positive control reached the pass level of 60% on day 3 (criterion:<14).

The following data could be determined for the test item N,N-Dimethyl-N-(2-methacryloyloxyethyl)-N-(3-sulfopropyl)-ammonium betaine; (SPE):

Lag phase: not detected

10-day-window:not detected

degradation at the end of 10-day-window: none

degradation at the end of the test: 8 %

Therefore, regardless of the 10-day-window, N,N-Dimethyl-N-(2-methacryloyloxyethyl)-N-(3-sulfopropyl)-ammonium betaine; (SPE) is classified as not readily biodegradable following OECD 301F/EU C.4-D.