Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 200-896-5 | CAS number: 75-73-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- S. typhimurium tester strain TA97a using ICR 191 as positive control was used as a substitute for strains TA97 and/or TA1537.
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Octafluorocyclobutane
- EC Number:
- 204-075-2
- EC Name:
- Octafluorocyclobutane
- Cas Number:
- 115-25-3
- Molecular formula:
- C4F8
- IUPAC Name:
- octafluorocyclobutane
Constituent 1
Method
- Target gene:
- hisD6610 (for S.typhimurium TA97a)
hisD3052 (for S.typhimurium TA98)
hisG46 (for S.typhimurium TA100)
hisG46 (for S.typhimurium TA1535)
trpE (for E.coli WP2)
Species / strain
- Species / strain / cell type:
- other: Salmonella typhimurium TA97a, TA98, TA100, TA1535 and Escherichia coli WP2
- Details on mammalian cell type (if applicable):
- S.typhimurium TA97a, TA98, TA100: deletion of uvrB; rfa; pKM101
S.typhimurium TA1535: deletion of uvrB; rfa
E.coli WP2: deletion of uvrA; pKM101
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor-induced rat liver S9
- Test concentrations with justification for top dose:
- Target concentrations: 0, 20, 40, 60, 80 and 100%
Trial 1:
- Without metabolic activation: 0, 25.6, 38.4, 57.2, 74.5, 89.0 %
- With metabolic activation: 0, 26.0, 40.6, 57.9, 68.4, 90.5 %
Trial 2:
- Without metabolic activation: 0, 26.7, 41.0, 54.5, 68.6, 90.0 %
- With metabolic activation: 0, 14.5, 42.0, 28.9, 67.5, 85.2 %
Controls
- Untreated negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 9,10-dimethylbenzanthracene
- 2-nitrofluorene
- sodium azide
- N-ethyl-N-nitro-N-nitrosoguanidine
- other: ICR 191 Acridine mutagen (ICR 191) and 2-aminoanthracene
Results and discussion
Test results
- Key result
- Species / strain:
- other: Salmonella typhimurium TA97a, TA98, TA100, TA1535 and Escherichia coli WP2
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- Under the conditions of this study, no evidence of mutagenic activity was detected in either trial with the test substance.
Based on the findings, H-24619 (octafluorocyclobutane) is concluded to be negative for the induction of mutagenicity in the bacterial reverse mutation test in Salmonella typhimurium and Escherichia coli in the presence and absence of the Aroclor-induced rat liver S9.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

Route: .live2