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EC number: 200-896-5 | CAS number: 75-73-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1996
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- other: OECS QSAR Toolbox
- Title:
- Unnamed
- Year:
- 1 996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Trifluoromethane
- EC Number:
- 200-872-4
- EC Name:
- Trifluoromethane
- Cas Number:
- 75-46-7
- Molecular formula:
- CHF3
- IUPAC Name:
- Trifluoromethane
Constituent 1
Method
- Target gene:
- hisD3052 (for S.typhimurium TA98)
hisG46 (for S.typhimurium TA100)
hisG46 (for S.typhimurium TA1535)
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- other: S. typhimurium TA1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- Type and composition of metabolic activation system:
- source of S9 : S9 mix from rat liver homogenates - Test concentrations with justification for top dose:
- 10, 50, 100%
Controls
- Negative solvent / vehicle controls:
- yes
- Remarks:
- air
- Positive controls:
- yes
- Positive control substance:
- other:
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Decrease in revertant colonies in strain TA1335 at 100% test article both with and without S9
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Decrease in revertant colonies in strain TA1335 at 100% test article both with and without S9
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Decrease in revertant colonies in strain TA1335 at 100% test article both with and without S9
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Decrease in revertant colonies in strain TA1335 at 100% test article both with and without S9
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Decrease in revertant colonies in strain TA1335 at 100% test article both with and without S9
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
Based on the 50% reduction in the number of revertants per plate in the both the presence and absence of metabolic activation at 100% of test substance in the toxicity assay, the top dose for the assay was set at 100% of test substance per plate in both the presence and absence of S9 activation.
In the mutation assay, there was no significant mutagenic response in any of the 5 strains, either in the presence or absence of metabolic activation. Strain TA98 at 25% of test substance exhibited a mean that was 2.1 times the mean of the 100% air control. However, this was due to a single high plate count and was not considered biologically relevant.
The positive controls exhibited a significant increase in mutant colonies in all strains both with and without S9. A reduction in the number of revertants per plate was observed with both 100% of test substance and 100% nitrogen in strains TA1538, TA98, and TA100 both with and without S9 indicating this effect was most likely due to oxygen deprivation and not test substance associated toxicity.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results: negative
Negative with and without metabolic activation.
This study and the conclusions which are drawn from it fulfill the quality criteria (validity, reliability, repeatability). - Executive summary:
The test substance was determined to be negative for mutagenic activity, both with and without metabolic activation, when tested at 10, 50, and 100% in a bacterial reverse mutation assay using Salmonella strains TA98, TA100, TA1535, TA1537 and TA1538.
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