Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: well documented study report, but no data concerning GLP compliance. screening test.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2004

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: flakes

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
Stock solution : the test item is dissolved directly in M4 reconstituted water. The obtained preparation is mechanically agitated for up to 24 hours in an attempt to completely dissolve the tes item.

test solutions are prepared by further dilution of the stock solutio with M4 or directly from the test item
ranges : 1 ; 10 ; 100 mg/L

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
animals were selected without preference and randomly assigned to test vessels.
20 animals per concentration divided into four replicate groups of five animals.

Study design

Test type:
static

Test conditions

Test temperature:
from 19.4 to 20.3 °C.
pH:
between 8.15 and 8.49.
Dissolved oxygen:
approx. 8.6 mg/L.
Nominal and measured concentrations:
nominal concentrations : 1 ; 10; 100 mg/L.
Reference substance (positive control):
yes

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Details on results:
no immobilisation of daphnia were observed at the higher concentration of 100 mg/L.

Applicant's summary and conclusion