Tetrahydrocyclopenta[c]pyrrole-1,3(2H,3aH)-dione

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: well documented study report, but no data concerning GLP compliance. screening test.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Tetrahydrocyclopenta[c]pyrrole-1,3(2H,3aH)-dione

EC Number:

227-285-6

EC Name:

Tetrahydrocyclopenta[c]pyrrole-1,3(2H,3aH)-dione

Cas Number:

5763-44-0

Molecular formula:

C7H9NO2

IUPAC Name:

4,5,6,6a-tetrahydro-3aH-cyclopenta[c]pyrrole-1,3-dione

Test material form:

solid: flakes

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

Stock solution : the test item is dissolved directly in M4 reconstituted water. The obtained preparation is mechanically agitated for up to 24 hours in an attempt to completely dissolve the tes item.

test solutions are prepared by further dilution of the stock solutio with M4 or directly from the test item
ranges : 1 ; 10 ; 100 mg/L

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

animals were selected without preference and randomly assigned to test vessels.
20 animals per concentration divided into four replicate groups of five animals.

Study design

Test type:

static

Test conditions

Test temperature:

from 19.4 to 20.3 °C.

pH:

between 8.15 and 8.49.

Dissolved oxygen:

approx. 8.6 mg/L.

Nominal and measured concentrations:

nominal concentrations : 1 ; 10; 100 mg/L.

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

dissolved

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

dissolved

Details on results:

no immobilisation of daphnia were observed at the higher concentration of 100 mg/L.