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EC number: 234-719-8 | CAS number: 12027-38-2
Analysis of the fresh test preparations at 0 and 72-Hours showed measured test concentrations of 5.0 and 5.2 mg/L. Analysis of the old test preparations at 24 and 96-Hours showed measured test concentrations of 5.1 and 5.4 mg/L. The measured concentrations were between 25% and 27% of the nominal concentrations, therefore the duplicate samples at 0 and 24-Hours were analyzed results from which confirmed the original results. Post test solubility samples were also prepared and analyzed at a nominal concentration of 20 mg/L in dechlorinated laboratory water which showed measured concentrations of 32 and 43% of the nominal concentration. Results of the chemical analysis show that there was a media effect which resulted in the low recovery of the test item. Given these results it was considered justifiable to base the results on the geometric mean measured test concentration to give a "worst case" analysis of the data
In a limit test, a nominal concentration of 20mg/L (determined as the EC50 in algae) of tungstosilicic acid at the start of the study had no adverse effects on rainbow trout during the 96 hours of the study. The measured analytical concentrations were found to decline substantially over the course of the study such that the geometric mean concentration was calculated at 5.8mg/L. Due to the differences in test media between this study and the Algal Growth Inhibition test performed on this test item (see chapter 6.1.3) the geometric mean measured test concentration of 5.8 mg/L obtained in dechlorinated tap water was considered to be equivalent to a concentration of 20 mg/L in algal culture medium. This study therefore showed that rainbow trout were not any more sensitive to the test item than algae
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