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Diss Factsheets
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EC number: 234-719-8 | CAS number: 12027-38-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- Limit study approach based on lowest value from non-vertebrate tests.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 12-wolframosilicic acid
- EC Number:
- 234-719-8
- EC Name:
- 12-wolframosilicic acid
- Cas Number:
- 12027-38-2
- Molecular formula:
- H.1/4O40SiW12
- IUPAC Name:
- tetrakis(hexaoxocyclotritungstoxane); silicic acid
- Test material form:
- solid
Constituent 1
- Specific details on test material used for the study:
- Identification: Tungstosilicic-acid
Physical state/Appearance: White Crystalline Solid
Batch: MKBR8664V
Purity: 65.0% w/w
Expiry Date: 26 August 2016
Storage Conditions: Room temperature in the dark over silica gel
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Sampling at 0, 24, and 96hrs
Test solutions
- Vehicle:
- no
- Details on test solutions:
- An amount of test item (400 mg) was dissolved in test water and the volume adjusted to 2 liters to give a 200 mg/L stock solution. This stock solution was dispersed in a final volume of 20 liters of test water to give the required test concentration of 20 mg/L.
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Source: Brow Well Fisheries Limited, Hebden, near Skipton, Yorkshire, UK.
- Length at study termination (length definition, mean, range and SD): 5.3 cm (sd = 0.3.
- Weight at study termination (mean and range, SD): 1.15 g (sd = 0.13.
ACCLIMATION
- Acclimation period: 10 days.
- Type and amount of food during acclimation: commercial trout pellets which were discontinued approximately 18 hours prior to the start of the definitive test.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 140mg/L as CaCO3
- Test temperature:
- 13C
- Dissolved oxygen:
- at least 10 mg O2/L.
- Nominal and measured concentrations:
- 20mg/L nominal
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass
- Type (delete if not applicable): covered
- Material, size, headspace, fill volume: 25-30 litre. Fill volume 20L.
- Aeration: yes
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.4 g bodyweight/liter.
TEST MEDIUM / WATER PARAMETERS (typical parameters for supply)
- Source/preparation of dilution water: Tap water, dechlorinated and partially softened.
- Metals(ug/L): Sb 0.11-0.46; Cd 0.08-0.17, Al<0.19; As<0.37; Cd<0.17; Cr<0.7, Cu<0.052; Fe 221; Pb 1.0; Mn 18; Ni 5.2;
- Pesticides: below LOD except Flutriafol <0.048ug/L, metaldehyde <0.03
- Chlorine (free):0.01-0.61mg/L
- Conductivity: 214-486uS/cm
- pH 6.9 - 8.1
OTHER TEST CONDITIONS
- Adjustment of pH: yes
- Photoperiod: 16/8 hrs light/dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality
TEST CONCENTRATIONS
- Single concentration based on EC50 from definitive tests on daphnia and algae (lowest figure). - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 5.8 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 20 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Analysis of the fresh test preparations at 0 and 72-Hours showed measured test concentrations of 5.0 and 5.2 mg/L. Analysis of the old test preparations at 24 and 96-Hours showed measured test concentrations of 5.1 and 5.4 mg/L. The measured concentrations were between 25% and 27% of the nominal concentrations, therefore the duplicate samples at 0 and 24-Hours were analyzed results from which confirmed the original results. Post test solubility samples were also prepared and analyzed at a nominal concentration of 20 mg/L in dechlorinated laboratory water which showed measured concentrations of 32 and 43% of the nominal concentration. Results of the chemical analysis show that there was a media effect which resulted in the low recovery of the test item. Given these results it was considered justifiable to base the results on the geometric mean measured test concentration to give a "worst case" analysis of the data
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Executive summary:
In a limit test, a nominal concentration of 20mg/L (determined as the EC50 in algae) of tungstosilicic acid at the start of the study had no adverse effects on rainbow trout during the 96 hours of the study. The measured analytical concentrations were found to decline substantially over the course of the study such that the geometric mean concentration was calculated at 5.8mg/L. Due to the differences in test media between this study and the Algal Growth Inhibition test performed on this test item (see chapter 6.1.3) the geometric mean measured test concentration of 5.8 mg/L obtained in dechlorinated tap water was considered to be equivalent to a concentration of 20 mg/L in algal culture medium. This study therefore showed that rainbow trout were not any more sensitive to the test item than algae
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