Anthracene

Biodegradation in water and sediment: simulation tests

Administrative data

Endpoint:

biodegradation in water and sediment: simulation tests

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study based on accepted scientific principles, limited documentation of the test method, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Microbial and photochemical degradation of polycyclic aromatic hydrocarbons in estuarine waters and sediments

Author:

Lee RF, Ryan C

Year:

1983

Bibliographic source:

Can. J. Fish. Aquat. Sci., 40(Suppl. 2), 86-94 (1983)

Materials and methods

Principles of method if other than guideline:

Biodegradation test in water and sediment slurry under shaking, using 14C-radiolabelled test substances.
Test conditions of Lee and Ryan (1983) did not resemble conditions required at the present for simulation tests.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Anthracene

EC Number:

204-371-1

EC Name:

Anthracene

Cas Number:

120-12-7

Molecular formula:

C14H10

IUPAC Name:

anthracene

Details on test material:

- Name of test material (as cited in study report): anthracene
- Specific activity (if radiolabelling): 76.6 MBq/mmol
- Locations of the label (if radiolabelling): 9-[14C]

Radiolabelling:

yes

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

other: water and sediment, adapted to different degree, marine

Details on source and properties of surface water:

Water and sediment were collected from one estuarine in Charleston, SC, contaminated with oil, and from a “cleaner” estuarine of Savannah, GA.
Further sediment samples were taken from MERL mesocosm tanks of Narragansett Bay, RI.

Duration of test (contact time):

>= 3 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

1 other: mg/kg sediment

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

2.5 other: mg/kg sediment

Based on:

test mat.

Initial test substance concentration 3

Initial conc.:

5 other: mg/kg sediment

Based on:

test mat.

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

1. Test with sediment, sediment-seawater slurry (Charleston and Savannah), consisting of 1 g of sediment and 50 ml of seawater in 125 mL bottles.
Test concentration of 14C-labelled anthracene: 2.5 mg/kg (no information whether dwt or wwt).
Temperature: 27 and 28 °C .
The flasks were carried out as triplicates.
Abiotic sterile control: with mercuric chloride added (10mg/g sediment)


2. Test with sediment, sediment-seawater slurry, sediments taken from MERL mesocosm tanks (Narragansett Bay, Rhode Island)
Temperature: 18 °C

Results and discussion

Mean total recovery

Compartment:

other: water / sediment, material (mass) balance

Remarks on result:

other: no data

Half-life of parent compound / 50% disappearance time (DT50)

Half-life of parent compound / 50% disappearance time (DT50) 1

Compartment:

entire system

DT50:

210 d

Type:

not specified

Remarks on result:

other: Savannah: "cleaner" area, 2.5 mg/kg, 27 °C

Half-life of parent compound / 50% disappearance time (DT50) 2

Compartment:

entire system

DT50:

57 d

Type:

not specified

Remarks on result:

other: Charleston: contaminated area, 2.5 mg/kg, 28 °C

Half-life of parent compound / 50% disappearance time (DT50) 3

Compartment:

entire system

DT50:

>= 79 - <= 99 d

Type:

not specified

Remarks on result:

other: Narragansett Bay: 1, 2.5 and 5 mg/kg in the control mesocosm at 18 °C

Half-life of parent compound / 50% disappearance time (DT50) 4

Compartment:

entire system

DT50:

>= 5 - <= 7 d

Type:

zero order

Remarks on result:

other: Narragansett Bay: 1, 2.5 and 5 mg/kg in the oil-pre-treated mesocosm at 18 °C

Any other information on results incl. tables

no further data