Sodium 1-amino-9,10-dihydro-4-[[4-[[methyl[(4-methylphenyl)sulphonyl]amino]methyl]phenyl]amino]-9,10-dioxoanthracene-2-sulphonate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14 - 15 Jul 1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

exposure period = 24 h

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Disodium 1-amino-9,10-dihydro-9,10-dioxo-4-[[4-[[2-(sulphonatooxy)ethyl]sulphonyl]phenyl]amino]anthracene-2-sulphonate

EC Number:

240-267-2

EC Name:

Disodium 1-amino-9,10-dihydro-9,10-dioxo-4-[[4-[[2-(sulphonatooxy)ethyl]sulphonyl]phenyl]amino]anthracene-2-sulphonate

Cas Number:

16102-99-1

Molecular formula:

C22H18N2O11S3.2Na C22H16N2Na2O11S3

IUPAC Name:

disodium 1-amino-9,10-dioxo-4-[(4-{[2-(sulfonatooxy)ethyl]sulfonyl}phenyl)amino]-9,10-dihydroanthracene-2-sulfonate

Test material form:

solid: particulate/powder

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

stock solution: 1% dissolved in deionized water
test concentrations: dilution of stock solution to respective concentreations in test vessels with medium

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain: Strauss
- Source: Dr. U. Noack-Laboratorium, Hildesheim
- Age at study initiation (mean and range, SD): less than 24 hours
- Feeding during test: twice daily
- Food type: dry food

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

24 h

Test conditions

Test temperature:

21.5°C

pH:

7.19 to 7.68

Dissolved oxygen:

7.8 to 8 mg/L

Salinity:

NA

Nominal and measured concentrations:

5800, 3240, 1800, 1000, 580, 324, 180, 100 mg test substance/L test water
the test substance was found to be stable over the test period

Details on test conditions:

TEST SYSTEM
- Test vessel: 50 ml beaker
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates):
- Biomass loading rate:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: own preparation according D!N 38412 part II, 8.2

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 12/12
- Light intensity: 10 µE/qm*s

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility at 24 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Test concentrations: 5800, 3240, 1800, 1000, 580, 324, 180, 100 mg/L
- Range finding study: yes

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: own preparation according to 8.2 test guide

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

900 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CI: 348 to 2328 mg/L

Results with reference substance (positive control):

EC50 was at 0.7 mg/L

Reported statistics and error estimates:

EC50 and CI calculation according to "Ausgewählte Methoden der Wasseruntersuchung" Band II (1982), PP 379-382

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 24-h EC50 to Daphnia magna was calculated to be 900 mg/L.