2,2'-vinylenebis[5-methylbenzoxazole]

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: screening test, other

Remarks:

COD

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1973

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: Internal standard guideline

Deviations:

no

Principles of method if other than guideline:

Internal SOP, see detailled description below

GLP compliance:

no

Inoculum or test system:

activated sludge, industrial (adaptation not specified)

Details on inoculum:

bacteria from the underrun of a sewage treatment plant were used.

Key result

Parameter:

other: BOD5

Value:

35

Sampling time:

5 d

Remarks on result:

other: BOD

Key result

Parameter:

BOD5

Value:

35 mg O2/g test mat.

Validity criteria fulfilled:

no

Interpretation of results:

not readily biodegradable

Conclusions:

BOD5 for FAT 65004 was found to be 35 mg/g O2 Taking the results together with the results of the COD test, the quotient for BOD5/COD is less 35/180= 0.028 indicationg that the substance is not readily biodegradable.

Reference 2

Endpoint:

biodegradation in water: screening test, other

Remarks:

COD

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1973

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

other: based on DIN 38409-H41-1

Version / remarks:

Internal standard method followed

Principles of method if other than guideline:

The part of a chemical substance that is able to be oxidized is determined. The sample is mixed with potassium dichromate (serving as oxidizing agent) and stirred for 2 hours in a sulfuric acid solution at elevated temperature. Subsequently, the excess of dichromate is determined quantitatively by titration with ammonium iron(II)-sulfate solution.

GLP compliance:

no

Inoculum or test system:

water (not specified)

Key result

Parameter:

other: COD measurement

Value:

180

Remarks on result:

other: COD measurement

Key result

Parameter:

COD

Value:

180 mg O2/g test mat.

Validity criteria fulfilled:

not specified

Interpretation of results:

other: COD

Conclusions:

COD for FAT 65004 was found to be 180 mg/g O2.

Executive summary:

COD for FAT 65004 was found to be 180 mg/g O2. Taking the results together with the results of the BOD test, the quotient for BOD5/COD is less 35/180= 0.028 indicationg that the substance is not readily biodegradable.

Reference 3

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))

GLP compliance:

no

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, industrial (adaptation not specified)

Details on inoculum:

activated sludge from an industrial sewage treatment plant was used

Duration of test (contact time):

28 d

Initial conc.:

30 mg/L

Based on:

ThOD

Key result

Parameter:

other: BOD

Value:

0

Sampling time:

28 d

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The test substance showed 0% biodegradation.

Executive summary:

The test substance showed 0% biodegradation under MITI - Test, OECD 302 C.

Description of key information

The test substance FAT 65004 was determined to be not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Additional information

In a key study the test substance FAT 65004 showed 0 % biodegradation under MITI - Test, OECD 302 C.

In a supporting study was performed to determine the biochemical oxygen demand of FAT 65004 by measuring the dissolved oxygen concentration before and after 5 days incubation at 20 °C. Based on the findings from the study, the BOD5 of FAT 65004 is 35 mg O2/g test material, and COD was noted to be 180 mg O2/g test material. The relevant BOD5/COD quotient is <0.5, therefore the substance is not indicative of rapid degradation.

Taking into consideration the data from key study and supporting studies, the test substance FAT 65004 is considered to be not biodegradable.

By the nature of their design and use, optical brighteners are not intended to be readily biodegradable as this would assist in the rapid destruction of the chemical, rendering it unfit for purpose. As such, it is accepted that such substances are not readily biodegradable under relevant environmental conditions. A published study (Pagga & Brown, 1986) describes the results of the testing of 87 dyestuffs in short-term aerobic biodegradation tests. The authors of this publication concluded that dyestuffs are very unlikely to show any significant biodegradation in such tests and that there seems little point in carrying out such test procedures’ on dyestuffs