3-isopropoxypropylamine

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test procedure according to national standards

Qualifier:

according to guideline

Guideline:

other: German industrial standard test guideline DIN 38 412, part L15.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The doses were chosen based on a range finding study.
- The product was added to the test water without any pretreatment

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Goldorfe
- Strain: Leuciscus idus (golden variety)
- Source: Fischzucht Paul Eggers, 2354 Hohenwestedt, Germany
- Length at study initiation: 7.7 cm (range: 6.9-8.9)
- Weight at study initiation: 4.3 g (range: 2.8 - 6.6)
- Feeding during test: no

ACCLIMATION
- Acclimation conditions (same as test or not): oil-free aerated and charcoal filtered tap water, flow-through system
- Water temperature: 20- 21 °C
- Duration of acclimation to test water and temperature: 3 days
- Type and amount of food: growing Feed F/B 50, SSNIFF Spezialdiaeten GmbH, Soest, Germany
- Feeding frequency: ad libitum.
- Health during acclimation (any mortality observed): 0.3%

HOUSING
- Housing water: charcoal filtered dechlorinated water in continuous flow-through
- Aeration: oilfree air
- Light regime: 16 : 8 hours light : dark
- Housing water properties:
- Hardness: about 2.5 mmol/L
- Oxygen concentration: > 60% of maximum saturation
- pH: about 8.0
- Water temperature: 13 - 23 °C
- Duration of housing: 5 months
- Medical treatment: four times with 0.05 mg/L malachite green chloride and twice with 10 mg/L tetracyclin hydrochloride
- Amount of feed: ad libitum
- Mortality during the last 2 weeks of housing: 2.4%

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

20 - 21 °C

pH:

7.4 - 10.6

Dissolved oxygen:

7.1 - 9.0 mg/L

Nominal and measured concentrations:

- Nominal test concentrations: 21.5, 46.4, 100, 215, 464, 1000 mg/L.
- Additionally, to study the effect of the high pH on the toxicity the concentration of 1000 mg/L was tested in parallel after pH-adjustment

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquaria (30 x 22 x 24 cm)
- Test volume: 10 L
- Aeration: slight aeration with oil-free air
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 4.3 g fish /L
- Feeding: withdrawl of food before exposure: 1 day

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, part 11
- Ca/Mg ratio: 4 : 1
- Intervals of water quality measurement: 1, 24, 48, 72, and 96 hours

OTHER TEST CONDITIONS
- Photoperiod: 16:8 hour light:dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, symptoms

Reference substance (positive control):

yes

Remarks:

Chloracetamid

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 87 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: apathy, tumbling, narcotic-like state of fish observed in test concentration of 100 mg/L
- No dead fish were found in the pH-adjusted test concentration of 1000 mg/L. Therefore, the observed effect may mainly be based on the shift in pH.

Results with reference substance (positive control):

- Results with reference substance valid? yes
- LC50: ca. 26 mg/L (48 h)

Sublethal observations / clinical signs:

Cumulative Mortality

Nominal conc.(mg/l)	Number of fish	Mortality (cumulated) after
		1 h	4 h	24 h	48 h	72 h	96 h
0	10	0	0	0	0	0	0
21.5	10	0	0	0	0	0	0
46.4	10	0	0	0	0	0	0
100	10	0	0	5	5	7	7
215	10	10	10	10	10	10	10
464	10	10	10	10	10	10	10
1000	10	10	10	10	10	10	10
1000*	10	0	0	0	0	0	0

*: pH-adjusted

No mortality was observed at 1000 mg/L if neutralised smaples were used.

pH values

Nominal conc.(mg/l)	pH values after
	1 h	24 h	48 h	72 h	96 h
0	7.7	7.4	7.5	7.6	7.8
0*	7.6	7.8	7.6	7.5	8.0
21.5	8.4	7.7	7.6	7.7	7.7
46.4	9.1	7.7	7.7	7.7	7.8
100	9.4	7.8	7.5	7.7	7.8
215	10.0
464	10.4
1000	10.6
1000*	7.7	7.7	7.7	7.5	7.5

* pH adjusted

Validity criteria fulfilled:

yes

Description of key information

In its acidic form, the substance has a 96h-LC50 of 83 mg/L according to an OECD 203 test. According to this same test, the substance is no longer toxic up to 1000 mg/L when neutralized. Therefore, the substance is not considered toxic or harmful to fish.