Registration Dossier

Physical & Chemical properties

Particle size distribution (Granulometry)

Administrative data

Endpoint:
particle size distribution (granulometry)
Data waiving:
study scientifically not necessary / other information available
Justification for data waiving:
the study does not need to be conducted because the substance is marketed or used in a non solid or granular form
Cross-referenceopen allclose all
Reason / purpose:
reference to same study
Reference
Endpoint:
appearance / physical state / colour
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From 2015-07-24 to 2016-06-24
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Qualifier:
no guideline available
GLP compliance:
not specified
Other quality assurance:
other: ISO 14001
Physical state at 20°C and 1013 hPa:
solid
Form:
other: waxy solid
Colour:
White
Substance type:
organic
Conclusions:
Cyastat SN is an organic solid substance, appearing under the form of white waxy solid at ambient temperature and pressure.
Executive summary:

The substance, Cyastat SN, was tested for several physico-chemical properties. In this context, the appearance of the sample was described, indicating that Cyastat SN is a white waxy solid substance

Reason / purpose:
reference to same study
Reference
Endpoint:
melting point/freezing point
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From 2015-07-24 to January 2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Qualifier:
according to
Guideline:
OECD Guideline 102 (Melting point / Melting Range)
Deviations:
no
GLP compliance:
not specified
Other quality assurance:
other: ISO 14001
Type of method:
differential scanning calorimetry
Melting / freezing pt.:
39 °C
Decomposition:
no

Melting pojnt / melting range of the test substance determined on 1st heat cycle.

Specimen No.

Onset (Melting Point) (°C)

Peak (°C)

Endset (°C)

Melting Range (°C)

Enthalpy (joules/g)

1

39.00

47.68

53.1

39 - 53

59.870

2

38.89

46.63

52.8

39 - 53

57.709

Specimen weights and appearance during DSC experiments.

Specimen No.

Initial specimen wt (mg)

Appearance

1

4.6870

Both specimens were a sticky white waxy solid before and after experiment with no discoloration observed.

2

7.6100

Conclusions:
The melting temperature of Cyastat SN was 39°C.
Executive summary:

Melting point of Cyastat SN has been determined according to OECD test guideline 102. Using Differential Scanning Calorimetry (DSC), the melting temperature of the test substance was determined to be equal to 39°C

Reason / purpose:
reference to same study
Reference
Endpoint:
boiling point
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From 2015-07-24 to Januaery 2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Qualifier:
according to
Guideline:
OECD Guideline 103 (Boiling Point)
Deviations:
no
GLP compliance:
not specified
Other quality assurance:
other: ISO 14001
Type of method:
differential scanning calorimetry
Decomposition:
yes
Decomp. temp.:
> 196 - < 220 °C

The test substance does not boil, instead it decomposes. Decomposition was confirmed by performing multiple DSC scans at different heating rates. The onset at 2.5°C/min is 195°C. Consequently, increasing the scan speed to 20°C/min delays the onset of decomposition to 220°C. Decomposition is a 2nd order reaction and is dependent upon the heating rate. Boiling point is a 1st order reaction independent of heating rate. Thus the different onset temperatures observed confirms that the thermal event is decomposition. The table below provides the onsets of decomposition at three different scan rates.

Onset of Decomposition for Test Substance.

Specimen No.

Onset (°C)

Heating Rate (°C/minute)

1

196

2.5

2

196

2.5

3

211

10

4

211

10

5

221

20

6

218

20

Specimen weights and appearance during DSC experiments.

Specimen N°

Initial specimen wt (mg)

Post run appearance

1

4.822

All of the specimens experienced a color change of white to brown. There was condensation of all specimens on top of the DSC pan lid through the pinhole confirming decomposition.

2

4.719

3

5.001

4

4.914

5

4.558

6

5.067

Conclusions:
The Cyastat SN decompose before boiling
Executive summary:

Boiling Point has been determined according to OECD guideline 103. Using Differential scanning calorimetry (DSC), it was showed that the Cyastat SN decompose before boiling.

Reason / purpose:
reference to same study
Reference
Endpoint:
density, other
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From 2017-07-24 to january 2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Qualifier:
according to
Guideline:
OECD Guideline 109 (Density of Liquids and Solids)
Deviations:
no
GLP compliance:
not specified
Other quality assurance:
other: ISO14001
Type of method:
pycnometer method
Type:
density
Density:
1.08 g/cm³
Temp.:
23.4 °C

Summary of Density Results, specimen weights, and room temperature during analysis.

Test Substance Specimen No.

Average true volume (n=10)

(mL)

Average Density (n=10) (g/mL)

S.D.

Initial specimen weight (g)

Final sample weight (g)

Temperature (°C)

1

5.9307

1.0812

0.0003

6.4123

6.4123

23.4

2

5.8536

1.0810

0.0003

6.3277

6.3276

23.4

Conclusions:
The density of Cyastat SN was 1.09 g/cm3 at 23.4°C
Executive summary:

Density of Cyastat SN has been determined according to OECD test guideline 103. Using a pycnometer method, the density of the test substance was determined to be 1.08 g/cm3 at 23.4 °C.

Reason / purpose:
reference to same study
Reference
Endpoint:
partition coefficient
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
test procedure in accordance with generally accepted scientific standards and described in sufficient detail
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Qualifier:
no guideline followed
Version / remarks:
See principles of method if other than guideline
Principles of method if other than guideline:
The octanol water partitioning coefficient was estimated using the solubility of the test substance in the pure phases (n-octanol and water). This is because the test substance is a salt and surface active which means neither the shake-flask method (OECD 107) nor the HPLC method (OECD 117) are suitable to determine the log POW of the test substance
GLP compliance:
not specified
Other quality assurance:
other: ISO14001
Type of method:
HPLC method
Partition coefficient type:
octanol-water
Analytical method:
liquid chromatography
Type:
log Pow
Partition coefficient:
> 0.32 - < 1.75
Temp.:
20 °C
pH:
6.8
Details on results:
The test substance is more soluble in octanol than in water and there was no undissolved test substance in concentrations prepared for concentrations less than 40.5 weight %. Concentrations at 10, 20, 30, and 40 weight % were clear solutions. Therefore, only three supernatants were collected on concentrations higher than 40 weight %. The three initial concentrations whereby undissolved test substance was observed were prepared at 45, 50, and 55 weight %. The supernatants were collected after 24, 48, and 72 hours equilibrium and diluted with THF. The LC/CAD results showed 40.5, 40.7, and 40.3 weight % respectively.
Conclusions:
The log Kow was determined to be in the range of 0.32 -1.75 depending on the concentration of the substance.
Executive summary:

Log Kow o the Cyastat SN was determined based on solubility of the test substance in water and in octanol. The solubility in Octanol and water were determinated by LC/CAD method.

Reason / purpose:
reference to same study
Reference
Endpoint:
vapour pressure
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From 2015-07-24 to 2016-06-24
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Qualifier:
according to
Guideline:
OECD Guideline 104 (Vapour Pressure Curve)
Deviations:
yes
Remarks:
a pan was used instead of a plate
GLP compliance:
not specified
Other quality assurance:
other: ISO 14001
Type of method:
effusion method: isothermal thermogravimetry
Test no.:
#1
Temp.:
50 °C
Vapour pressure:
0.163 Pa
Test no.:
#2
Temp.:
60 °C
Vapour pressure:
0.186 Pa
Test no.:
#3
Temp.:
70 °C
Vapour pressure:
0.21 Pa
Test no.:
#4
Temp.:
80 °C
Vapour pressure:
0.21 Pa

The evaporation rate of the test substance was determined at 5 temperatures between 60 and 175°C. The equation in the plot above was used to calculate the vapor pressure. See results in Table 8.2. The calculated vapor pressure based on evaporation rates was plotted against temperature in order to derive the vapor pressure equation. The calculated vapor pressures were done in mm Hg because all calibration tables were done in this unit. After, the calculated vapor pressures for the test substance were converted to Pascal as per OECD Guideline.

Table 8.2. Experimental evaporation rates of test substance with calculated vapor pressure.

Temp. (°C)

Evaporation Rate (µg/min)

log Evaporation Rate (µg/min)

Temp. (1/K)

Log Vapor Pressure (mmHg)

Calculated Vapor Pressure (mm Hg)

Calculated Vapor Pressure (Pascal)

60

0.0684

-1.164943898

0.003002

-2.855596556

0.001394

0.1859

80

0.0875

-1.057991947

0.002832

-2.745346474

0.001797

0.2396

50

0.0601

-1.221125528

0.003095

-2.913510686

0.001220

0.1627

70

0.0770

-1.113509275

0.002914

-2.802575817

0.001576

0.2101

 

Conclusions:
The vapour pressure of Cyastat SN was determined to be equal to 0.16 Pa at 50°C.
Executive summary:

Density of Cyastat SN has been determined according to OECD test guideline 104. Using TGA method, the vapour pressure of the test substance was determined to be 0.16 Pa at 50 °C.

Reason / purpose:
reference to same study
Reference
Endpoint:
water solubility
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Qualifier:
according to
Guideline:
OECD Guideline 105 (Water Solubility)
GLP compliance:
not specified
Other quality assurance:
other: ISO 14001
Type of method:
flask method
Water solubility:
< 0.5 other: w/%
Conc. based on:
test mat.
Incubation duration:
24 h
Temp.:
20 °C
pH:
6.8

Water solubility results done by LC/CAD contingent on initial concentrations at 20 °C

*Initial concentration (Weight %)

Solubility

(Weight %)

24 hours

48 hours

72 hours

Mean

Maximum Difference

**(M.D)

1.0

0.73

0.70

0.72

0.717

4.19

4.0

2.57

2.54

2.61

2.57

2.72

16.0

10.54

10.44

10.76

10.6

3.02

22.2

13.78

13.56

13.68

13.7

1.61

39.0

19.69

19.91

18.80

19.5

5.70

*This is the initial concentration of the test substance in water. At all of these concentrations there was undissolved test substance. The pH of all collected supernatants was 6.8. 

**We calculate the M.D. as follows:

                               M.D. = ((highest value - lowest value) / mean value) x 100

where: mean value is the mean of the highest and lowest value

According to OECD 105, the maximum difference should be <15% “If the concentrations measured in at least the two last vessels do not differ by more than 15%, the test is satisfactory.” However, this statement is valid for very pure test substances consisting of single components. This test substance consists of multiple components varying in water solubility. The results are not increasing with additional equilibrium time, thus analysis is satisfactory.

Conclusions:
The water solubility of Caystat SN was estimated to be less than 0.5%/w
Executive summary:

Water solubility of Caystat SN has been determined according to OECD test guideline 105. Using LC/CAD, the water solubility of the test substance was determined to be less than 0.5%/w at 20 °C.

Reason / purpose:
reference to same study
Reference
Endpoint:
surface tension
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From 2015-07-24 to January 2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Qualifier:
according to
Guideline:
OECD Guideline 115 (Surface Tension of Aqueous Solutions)
GLP compliance:
not specified
Other quality assurance:
other: ISO 14001
Type of method:
ring method
Specific details on test material used for the study:
A 1 g/L test solution was prepared of the test substance in distilled water. Two aliquots were poured into test vessels immediately after preparation.
A 1 g/L test solution was used after determining that solubility of the test substance in water exceeds 0.3 wt%. The test solution is water clear and less than 200 mPa s (200 centipoise).
Surface tension:
42.7 mN/m
Temp.:
20 °C
Conc.:
1 g/L

Surface Tensions of 1 g/L Test Substance in Water at 20°C.

Surface Tension of test solution #1 (mN/m)

Surface Tension of test solution #2 (mN/m)

Equilibrium Time after pouring into test vessel

42.7

42.7

20 seconds

42.6

42.8

90 seconds

42.7

42.7

180 seconds

42.8

42.7

300 seconds

The surface tension of the above test solution does not have any dependence on time.

Conclusions:
The surface tension of Cyastat SN was 42.7 mN/m at 20°C
Executive summary:

Surface tension of Cyastat SN has been determined according to OECD test guideline 115. Using a Kruss digital Tensiometer K-10 and Dunouy ring method, the surface tension of the test substance was determined to be equal to 42.7 mN/m at 20°C

Data source

Materials and methods

Results and discussion

Applicant's summary and conclusion