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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
other: FDA test guideline 4.11, Freshwater Fish Acute Toxicity, Mar 1987
Deviations:
not specified
Principles of method if other than guideline:
Comparable to OECD 203
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 0.5 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 0.5 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Sublethal observations / clinical signs:

10% mortality was observed in the highest concentration of 0.5 mg/L (saturated solution) at 48 h. This is within the accepted mortality rate of the controls, therefore it is not considered to be a substance related effect. No fish died in the other test and control solution.

Validity criteria fulfilled:
yes
Conclusions:
The LC50 (96 h) was >0.5 mg/L
Executive summary:

In the acute LC50 study with rainbow trout (Oncorhynchus mykiss), 10 animals/concentration were exposed to 0.005, 0.042 and 0.507 mg/L estradiol in tap water for a period of 96 hours. Mortality and visual abnormalities were recorded at ca. 3, 6, 24, 48, 72 and 96 hours. 10% mortality was observed in the highest concentration of 0.5 mg/L (saturated solution) at 48 h.


This is within the accepted mortality rate of the controls, therefore it is not considred to be a substance related effect.


No fish died in the other test and control solution resulting in a LC50 (96 h) of >0.5 mg/L.

Description of key information

Acute LC50 study with rainbow  trout (Onkorhychus mykiss), 96h, 10 fish/concentration;
Hydrographical parameters: t = 12-13 C; pH =7.8-8.2; O2 = >=78%; Total hardness: 277-290 mg/L; test concentrations: 0.005, 0.042, 0.507 mg/L
Results: No mortality or visuable abnormality. The LC50/48 h was >0.5 mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Dose descriptor:
LC50
Effect concentration:
> 0.5 mg/L

Additional information

Study was conducted according to GLP (report A05662)