Methoxyacetic acid

Repeated dose toxicity: oral

Administrative data

Endpoint:

repeated dose toxicity: oral

Remarks:

other: repeated dose (eight daily doses)

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: abstract available, no information about purity of the test substance

Data source

Reference

Reference Type:

publication

Title:

Toxicity of methoxyacetic acid in male rats

Author:

Miller RR, Carreon RE, Young JT, McKenna MJ

Year:

1982

Bibliographic source:

Fundam Appl Toxicol, 2: 158-160.

Materials and methods

GLP compliance:

not specified

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Methoxyacetic acid

EC Number:

210-894-6

EC Name:

Methoxyacetic acid

Cas Number:

625-45-6

Molecular formula:

C3H6O3

IUPAC Name:

2-methoxyacetic acid

Constituent 2

Reference substance name:

2-methoxyacetic acid

IUPAC Name:

2-methoxyacetic acid

Details on test material:

No data given.

Test animals

Species:

rat

Strain:

Fischer 344

Sex:

male

Details on test animals or test system and environmental conditions:

No data given.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

not specified

Details on oral exposure:

No data given.

Analytical verification of doses or concentrations:

not specified

Frequency of treatment:

eight daily doses.

Doses / concentrations

Remarks:

Doses / Concentrations:
0, 30, 100 or 300 mg/kg bw
Basis:
no data

No. of animals per sex per dose:

Five animals.

Results and discussion

Results of examinations

Details on results:

At 100 and 300 mg/kg bw/d, there was a significant dose-dependent reduction in the absolute and relative thymus weight, the erythrocyte count, and the haemoglobin and haematocrit level (p < 0.05 in each case). In the high dose group from the 5th day of administration there were significant reductions in body weight, absolute and relative spieen and testes weights, and leucocyte count (p < 0.05 in each case). The following effects were seen at 100 and 300 mg/kg bw/d, on histological examination: loss of thymocytes in the thymic cortex and atrophy of the germ cells in the seminiferous tubules („degeneration of testicular germinal epithelium"). The high dose led to reduced cell density in the bone marrow and giant cell formation in the testes. Granulopoiesis, erythropoiesis and thrombopoiesis were not affected. The spieen was not examined in this study.

Target system / organ toxicity

Critical effects observed:

not specified

Applicant's summary and conclusion

Executive summary:

Male F344 rats received 8 daily gavage administrations of 0, 30, 100 or 300 mg Methoxyacetic acid/kg bw over a 2-week period. At the two highest dose levels, absolute and relative thymus weights, RBC, Hb and Hct values were significantly decreased. In the 300 mg/kg bw group, there was a decrease of body weight, absolute and relative spleen and testicular weights, and leukopenia. Cellularity of thymus core and germinal epithelium were affected at 100 mg/kg bw and above. Testicular giant cells and reduced cellularity in bone marrow were observed at 300 mg/kg bw, whereas doses of 30 mg/kg bw produced no effects within the observation period (Miller et al., 1982).