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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
test procedure in accordance with generally accepted scientific standards and described in sufficient detail
Justification for type of information:
Data is from study report.
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
Short term toxicity to Scenedesmus subspicatus was carried out for 96 hrs.
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material : 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide
- Molecular formula : C12H13N3O2S
- Molecular weight : 263.32 g/mol
- Smiles notation : O=S(=O)(c1ccc(cc1)Nc1ccc(cc1)N)N
- InChl : 1S/C12H13N3O2S/c13-9-1-3-10(4-2-9)15-11-5-7-12(8-6-11)18(14,16)17/h1-8,15H,13H2,(H2,14,16,17)
- Substance type : Organic
- Physical state : Solid
Analytical monitoring:
not specified
Details on sampling:
- Concentrations: Test chemical concentration used for the study were 0, 1.953, 3.906, 7.813, 15.625, 31.25, 62.5, 125 and 250 mg/l, respectively.
- Sampling method: The suspension were made from the stock solutions (1000 mg/l).
Vehicle:
not specified
Test organisms (species):
other: Scenedesmus subspicatus
Details on test organisms:
TEST ORGANISM
- Common name: Scenedesmus subspicatus
- Strain: SAG 88.81
- Other: The algae culture is set at 10,000 cells/ml.
Test type:
not specified
Water media type:
freshwater
Total exposure duration:
96 h
Test temperature:
20⁰C (293 K)
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer
- Material, size, headspace, fill volume: Nominal and net volume of the test vessel were 250 and 100 ml, respectively.
- Equipment settings: GAIN X 1
- Self-staining of the sample: reddish brown

GROWTH MEDIUM
- Standard medium used: Yes, OECD medium was used as a test medium during the study.
Reference substance (positive control):
not specified
Key result
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
152.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
not specified
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
37.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
not specified
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
21.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
not specified
Key result
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
21.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
not specified

Experimental results   Measuring time: 96 h

Conc. in mg/l

Fluorometer values

% of the control

Inoculated

Uninoculated

Difference

1.953

3.906

7.813

15.625

31.25

62.5

125

250

Control

790

735

731

626

229

101

70

33

957

1

0

1

1

3

5

8

15

1

789

735

730

625

226

96

62

18

82.51

75.82

76.34

65.36

23.61

10.04

6.48

1.88

EC20 = 2.68 mg/l

EC50 = 21.4 mg/l

EC90 = 83.2 mg/l

Conc. in ml/l

Fluorometer units

Standard

deviation

Variance in %

Single value

Mean

Net value

1.953

Inoculated

 

 

Uninoculated

 

3.906

Inoculated

 

 

Uninoculated

 

7.813

Inoculated

 

 

Uninoculated

 

15.625

Inoculated

 

 

Uninoculated

 

31.25

Inoculated

 

 

Uninoculated

 

62.5

Inoculated

 

 

Uninoculated

 

125

Inoculated

 

 

Uninoculated

 

250

Inoculated

 

 

Uninoculated

 

Blank values

Inoculated

 

 

Uninoculated

 

 

 

 

 

 

821.9

758

817

753.4

0.8

1.4

733.8

741.6

710.4

716.4

0.8

1

760.5

688.3

740.4

734.9

2.7

1.2

665.5

652.9

625.9

561.5

1.1

1.3

320.7

201.4

185.6

210.4

3.9

3.7

112.

94

98.8

100.4

6.7

5.1

72.8

67.7

64.9

75.3

8.6

8.2

30.2

37.7

32.1

32.4

14.7

15.5

874.5

1037.3

985

933.2

1.7

0.9

790

 

 

 

1

 

725

 

 

 

0

 

731

 

 

 

1

 

626

 

 

 

1

 

229

 

 

 

3

 

101

 

 

 

5

 

70

 

 

 

8

 

33

 

 

 

15

 

957

 

 

1

789

 

 

 

 

 

725

 

 

 

 

 

730

 

 

 

 

 

625

 

 

 

 

 

226

 

 

 

 

 

96

 

 

 

 

 

62

 

 

 

 

 

18

34.5

 

 

 

 

 

14.59

 

 

 

 

 

30.53

 

 

 

 

 

46.34

 

 

 

 

 

61.6

 

 

 

 

 

7.68

 

 

 

 

 

4.73

 

 

 

 

 

3.22

 

 

 

 

 

69.77

4.37

 

 

 

 

 

2.01

 

 

 

 

 

4.18

 

 

 

 

 

7.4

 

 

 

 

 

26.86

 

 

 

 

 

7.58

 

 

 

 

 

6.74

 

 

 

 

 

9.72

 

 

 

 

 

7.29

The standard deviation is determined from the individual values.

Experimental results   Measuring time: 72 h

Conc. in mg/l

Fluorometer values

% of the control

Inoculated

Uninoculated

Difference

1.953

3.906

7.813

15.625

31.25

62.5

125

250

Control

507

437

417

437

166

88

67

34

658

1

0

1

1

2

5

7

13

1

506

437

416

436

184

83

80

21

 

77.03

86.52

83.33

88.37

24.97

12.64

9.13

3.12

EC20 = ----

EC50 = 21.8 mg/l

EC90 = 109.5 mg/l

Conc. in ml/l

Fluorometer units

Standard

deviation

Variance in %

Single value

Mean

Net value

1.953

Inoculated

 

 

Uninoculated

 

3.906

Inoculated

 

 

Uninoculated

 

7.813

Inoculated

 

 

Uninoculated

 

15.625

Inoculated

 

 

Uninoculated

 

31.25

Inoculated

 

 

Uninoculated

 

62.5

Inoculated

 

 

Uninoculated

 

125

Inoculated

 

 

Uninoculated

 

250

Inoculated

 

 

Uninoculated

 

Blank values

Inoculated

 

 

 

Uninoculated

 

 

 

 

 

 

532.7

485.8

525.4

485.7

0.8

1.8

453.7

411.1

449.4

437.5

0.8

1

437.4

425.5

426.7

381.1

1.4

0.9

359.9

392.1

412.8

594.2

13

1.4

171.3

132.2

150.7

211.3

1.8

3.8

95.3

80.2

86.1

93.1

5.5

5.6

72.2

63.8

65.3

67.7

8

7.8

34.9

33.3

38

33.7

13.5

13.4

595.8

623.1

720.9

693.5

0.9

1.5

507

 

 

 

1

 

437

 

 

 

0

 

417

 

 

 

1

 

437

 

 

 

1

 

166

 

 

 

2

 

88

 

 

 

5

 

67

 

 

 

7

 

34

 

 

 

13

 

658

 

 

1

506

 

 

 

 

 

437

 

 

 

 

 

416

 

 

 

 

 

436

 

 

 

 

 

164

 

 

 

 

 

83

 

 

 

 

 

60

 

 

 

 

 

21

25.18

 

 

 

 

 

18.15

 

 

 

 

 

24.96

 

 

 

 

 

106.37

 

 

 

 

 

33.84

 

 

 

 

 

6.88

 

 

 

 

 

3.67

 

 

 

 

 

2.13

 

 

 

 

 

58.37

4.96

 

 

 

 

 

4.37

 

 

 

 

 

5.98

 

 

 

 

 

24.44

 

 

 

 

 

20.4

 

 

 

 

 

7.76

 

 

 

 

 

5.46

 

 

 

 

 

6.08

 

 

 

 

 

3.87

Experimental results   Measuring time: 48 h

Conc. in mg/l

Fluorometer values

% of the control

Inoculated

Uninoculated

Difference

1.953

3.906

7.813

15.625

31.25

62.5

125

250

Control

251

213

190

186

146

83

65

39

264

1

0

1

1

2

5

7

10

1

250

213

189

185

144

78

58

29

 

84.81

80.87

71.75

74.03

54.67

29.81

22.02

11.01

 

 

EC20 = 4.28 mg/l

EC50 = 37.1 mg/l

EC90 = ----

Conc. in ml/l

Fluorometer units

Standard

deviation

Variance in %

Single value

Mean

Net value

1.953

Inoculated

 

 

Uninoculated

 

3.906

Inoculated

 

 

Uninoculated

 

7.813

Inoculated

 

 

Uninoculated

 

15.625

Inoculated

 

 

Uninoculated

 

31.25

Inoculated

 

 

Uninoculated

 

62.5

Inoculated

 

 

Uninoculated

 

125

Inoculated

 

 

Uninoculated

 

250

Inoculated

 

 

Uninoculated

 

Blank values

Inoculated

 

 

Uninoculated

 

 

 

 

 

 

263.6

244.7

270.5

228.5

1.3

1.4

229.2

210.8

206.6

208.1

1.4

0.4

187.2

203.1

195.7

176.4

1.3

1.2

234.1

204.8

178.7

168.4

1.1

1.4

145.2

165.4

136.1

138.5

2.2

1.9

78.3

77.1

81.5

95.9

5.1

5

65.3

61.6

66.4

68.2

7.9

7.1

37.4

43.7

41.6

36.7

9.7

10.8

232.6

260.1

268.9

298

1.9

1.1

251

 

 

 

1

 

213

 

 

 

0

 

190

 

 

 

1

 

198

 

 

 

1

 

145

 

 

 

2

 

83

 

 

 

5

 

65

 

 

 

7

 

39

 

 

 

10

 

204

 

 

1

250

 

 

 

 

 

213

 

 

 

 

 

188

 

 

 

 

 

195

 

 

 

 

 

144

 

 

 

 

 

78

 

 

 

 

 

58

 

 

 

 

 

29

18.99

 

 

 

 

 

10.48

 

 

 

 

 

11.48

 

 

 

 

 

29.39

 

 

 

 

 

13.3

 

 

 

 

 

8.67

 

 

 

 

 

2.79

 

 

 

 

 

3.36

 

 

 

 

 

26.84

7.54

 

 

 

 

 

4.91

 

 

 

 

 

8.02

 

 

 

 

 

14.35

 

 

 

 

 

9.09

 

 

 

 

 

10.42

 

 

 

 

 

4.26

 

 

 

 

 

8.42

 

 

 

 

 

10.17

Experimental results   Measuring time: 24 h

Conc. in mg/l

Fluorometer values

% of the control

Inoculated

Uninoculated

Difference

1.953

3.906

7.813

15.625

31.25

62.5

125

250

Control

78

64

67

89

61

53

47

36

81

2

1

1

1

1

1

4

7

1

76

63

66

68

60

52

43

28

 

85.12

78.85

82.8

85.11

75.09

65.08

53.82

36.3

 

 

EC20 = 3.77 mg/l

  5.1 mg/l

             23.6 mg/l

EC50 = 152.2 mg/l

EC90 = ----

Conc. in ml/l

Fluorometer units

Standard

deviation

Variance in %

Single value

Mean

Net value

1.953

Inoculated

 

 

Uninoculated

 

3.906

Inoculated

 

 

Uninoculated

 

7.813

Inoculated

 

 

Uninoculated

 

15.625

Inoculated

 

 

Uninoculated

 

31.25

Inoculated

 

 

Uninoculated

 

62.5

Inoculated

 

 

Uninoculated

 

125

Inoculated

 

 

Uninoculated

 

250

Inoculated

 

 

Uninoculated

 

Blank values

Inoculated

 

 

Uninoculated

 

 

 

 

 

 

88.7

78.6

82.8

85.3

2

2.9

68.9

61.5

63.1

66.1

1.1

1.2

64.4

77.5

82.2

86.3

2.2

1.4

83.9

81.8

66.9

67

1.7

1.1

58.8

57.2

62.1

66.1

1.1

1

48.9

49.8

55.3

61.3

2.7

1.2

45.6

45.1

45.6

52.2

5.1

3.2

35.8

33.8

41.5

34.3

7.4

7

73.3

78.8

93.8

80.7

1.7

1.9

78

 

 

 

2

 

64

 

 

 

1

 

67

 

 

 

1

 

68

 

 

 

1

 

61

 

 

 

1

 

53

 

 

 

1

 

47

 

 

 

4

 

36

 

 

 

7

 

81

 

 

1

76

 

 

 

 

 

63

 

 

 

 

 

66

 

 

 

 

 

68

 

 

 

 

 

60

 

 

 

 

 

52

 

 

 

 

 

43

 

 

 

 

 

29

10

 

 

 

 

 

3.28

 

 

 

 

 

6.81

 

 

 

 

 

9.64

 

 

 

 

 

3.94

 

 

 

 

 

6.02

 

 

 

 

 

3.39

 

 

 

 

 

3.43

 

 

 

 

 

8.69

12.77

 

 

 

 

 

5.05

 

 

 

 

 

10.07

 

 

 

 

 

13.8

 

 

 

 

 

6.45

 

 

 

 

 

11.18

 

 

 

 

 

7.2

 

 

 

 

 

9.4

 

 

 

 

 

10.64

Experimental results   Measuring time: 0 h

Conc. in mg/l

Fluorometer values

% of the control

Inoculated

Uninoculated

Difference

1.953

3.906

7.813

15.625

31.25

62.5

125

250

Control

25

23

22

23

25

25

25

29

25

2

2

1

2

2

1

4

6

3

23

21

21

21

23

24

21

23

 

102.79

93.85

93.85

93.85

102.79

107.26

93.85

102.79

 

Validity criteria fulfilled:
not specified
Conclusions:
Based on the effect of test chemical 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide on test organism Scenedesmus subspicatus, the 24, 48, 72 and 96 hrs EC50 value was determined to be 152.2, 37.1, 21.8 and 21.4 mg/l, respectively.
Executive summary:

Short term toxicity to Scenedesmus subspicatus was carried out for 96 hrs.The study was based on the effects of the test compound 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide(CAS no. 16803 -97 -7) on Scenedesmus subspicatus in a fresh water system at a temperature of 20⁰C (293 K). Scenedesmus subspicatus SAG 88.81 was used as a test organism. The algae culture is set at 10,000 cells/ml.The suspension were made from the stock solutions (1000 mg/l). Test chemical nominal concentrations used for the study were 0, 1.953, 3.906, 7.813, 15.625, 31.25, 62.5, 125 and 250 mg/l, respectively. Standard OECD medium was used as a test medium during the study. Based on the effect of test chemical 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide on test organism Scenedesmus subspicatus, the 24, 48, 72 and 96 hrs EC50 value was determined to be 152.2, 37.1, 21.8 and 21.4 mg/l, respectively.Thus, based on the 96 hr EC50 value (21.4 mg/l), it can be concluded that the substance 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide can be considered as toxic to aquatic organisms and thus can be considered to be classified in aquatic chronic category 3 as per the CLP classification criteria.

Description of key information

Short term toxicity to Scenedesmus subspicatus was carried out for 96 hrs (Sustainability Support Services (Europe) AB, DUU/WL-A 520, 1987). The study was based on the effects of the test compound 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide(CAS no. 16803 -97 -7) on Scenedesmus subspicatus in a fresh water system at a temperature of 20⁰C (293 K). Scenedesmus subspicatus SAG 88.81 was used as a test organism. The algae culture is set at 10,000 cells/ml.The suspension were made from the stock solutions (1000 mg/l). Test chemical nominal concentrations used for the study were 0, 1.953, 3.906, 7.813, 15.625, 31.25, 62.5, 125 and 250 mg/l, respectively. Standard OECD medium was used as a test medium during the study. Based on the effect of test chemical 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide on test organism Scenedesmus subspicatus, the 24, 48, 72 and 96 hrs EC50 value was determined to be 152.2, 37.1, 21.8 and 21.4 mg/l, respectively.Thus, based on the 96 hr EC50 value (21.4 mg/l), it can be concluded that the substance 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide can be considered as toxic to aquatic organisms and thus can be considered to be classified in aquatic chronic category 3 as per the CLP classification criteria.

Key value for chemical safety assessment

EC50 for freshwater algae:
21.4 mg/L

Additional information

Short term toxicity to Scenedesmus subspicatus was carried out for 96 hrs (Sustainability Support Services (Europe) AB, DUU/WL-A 520, 1987). The study was based on the effects of the test compound 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide(CAS no. 16803 -97 -7) on Scenedesmus subspicatus in a fresh water system at a temperature of 20⁰C (293 K). Scenedesmus subspicatus SAG 88.81 was used as a test organism. The algae culture is set at 10,000 cells/ml.The suspension were made from the stock solutions (1000 mg/l). Test chemical nominal concentrations used for the study were 0, 1.953, 3.906, 7.813, 15.625, 31.25, 62.5, 125 and 250 mg/l, respectively. Standard OECD medium was used as a test medium during the study. Based on the effect of test chemical 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide on test organism Scenedesmus subspicatus, the 24, 48, 72 and 96 hrs EC50 value was determined to be 152.2, 37.1, 21.8 and 21.4 mg/l, respectively.Thus, based on the 96 hr EC50 value (21.4 mg/l), it can be concluded that the substance 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide can be considered as toxic to aquatic organisms and thus can be considered to be classified in aquatic chronic category 3 as per the CLP classification criteria.