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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Oct 25, 2016 - Dec 19:2016
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
OECD 202:2004
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
yes
Test organisms (species):
Daphnia magna
Details on test organisms:
- Source: National Chiayi University Freshwater Biological Resource Center
- Age: less than 24 hours old were derived form the same stock and were not first progeny.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
20 °C
Details on test conditions:
- Test volume: 30 mL
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Photoperiod: 16-hr light: 8-hr dark
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
2.57 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

In range-finding test, the 0% of control organisms were immobilised at the end of the test and the dissolved oxygen concentration of control and test groups during the test was 8.29-8.44 mg O2/L. The initial pH measurements did not differ from the final measurements by more than 1.5 units in each test. The results of the range-finding test showed that EC50 was 0.24 mg/L.

In definitive test, the results showed below:

 

Table 1. The numbers immobilized forthe definitive teston day 1 and 2

Days

Group

(mg/L)

Numbers of no immobilised

Numbers of activity immobilised

Numbers of discoloration

Numbers of trapping at surface of water

Day1

Control group

0.00

20

0

0

0

Test group

4.00

8

12

0

0

1.82

13

7

0

0

0.83

17

3

0

0

0.38

20

0

0

0

0.17

20

0

0

0

0.08

20

0

0

0

0.04

20

0

0

0

Day2

Control group

0.00

20

0

0

0

Test group

4.00

5

15

0

0

1.82

12

8

0

0

0.83

17

3

0

0

0.38

18

2

0

0

0.17

19

1

0

0

0.08

18

2

0

0

0.04

20

0

0

0

Table 2. The percentages immobilized for the definitive test

Group

(mg/L)

Percentages immobilized (%)

Day 0

Day 1

Day 2

Control group

0.00

0.00

0.00

0.00

Test group

4.00

0.00

60.00

75.00

1.82

0.00

35.00

40.00

0.83

0.00

15.00

15.00

0.38

0.00

0.00

10.00

0.17

0.00

0.00

5.00

0.08

0.00

0.00

10.00

0.04

0.00

0.00

0.00

Table 3. EC50 and 95% confidence interval for the definitive test

Parameter

Concentration

(mg/L)

EC50

2.57

95% confidence interval-lower

2.27

95% confidence interval-upper

2.92

Table 4. Temperature, dissolved oxygen and pH value for the definitive test

Group

(mg/L)

Day 0

Day 2

Temperature

(°C)

Dissolved

oxygen

(mg/L)

pH

value

Temperature

(°C)

Dissolved

oxygen

(mg/L)

pH

value

Control group

0.00

20.0

8.75 ± 0.14

7.49 ± 0.06

20.0

8.85 ± 0.04

6.88 ± 0.10

Test group

4.00

8.66 ± 0.09

7.57 ± 0.05

8.93 ± 0.05

7.02 ± 0.06

Validity criteria fulfilled:
yes
Conclusions:
According to OECD 202 test method, the 48-hr EC50 of Everlan SL65 was 2.57 mg/L.
Executive summary:

This test using the procedures outlined in the SuperLab Study Plan for M62-151100108002EN which is based on the SOP for the OECD 202 (SOPE-505) and OECD 202 (OECD, 2004). The results of this OECD 202 test for Everlan SL65 show that all validity criteria were met. The EC50 was 0.24 mg/L in the range-finding test, so the definitive test was conducted. The concentrations were 0.04, 0.08, 0.17, 0.38, 0.83, 1.82 and 4.00 mg/L in the definitive test. Under the conditions of this test, the 48-hr EC50 of Everlan SL65 was 2.57 mg/L.

Description of key information

The 48-hr EC50 of Everlan SL65 was 2.57 mg/L for Daphnia magna (OECD TG202).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
2.57 mg/L

Additional information

In both range-finding and definitive test, not more than 10 % of the Daphnia were immobilised in the control and the dissolved oxygen concentration of control and test groups at the end of the test was ≥3 mg O2/L. All test validity criteria were met. The pH of the test solutions was within the ranges outlined in the OECD 202 test guideline. Based on the results derived from the range-finding test, there should conduct definitive test. Therefore, the concentrations set between 0.04 and 4.00 mg/L in the definitive test. Under the conditions of this test, the 48-hr EC50 of Everlan SL65 was 2.57 mg/L.