Cyclopropanecarboxylic acid, [(3S,4R,4aR,6S,6aS,12R,12aS,12bS)-3-[(cyclopropylcarbonyl)oxy]-1,3,4,4a,5,6,6a,12,12a,12b-decahydro-6,12-dihydroxy-4,6a,12b-trimethyl-11-oxo-9-(3-pyridinyl)-2H,11H-naphtho[2,1-b]pyrano[3,4-e]pyran-4-yl]methyl ester

Long-term toxicity to aquatic invertebrates

Description of key information

Freshwater:

In a 7-day flow-through toxicity laboratory study (ABC Laboratories, 704056, 2014) with Ceriodaphnia dubia the NOEC for the number of live young per surviving adult were 181 ng/L, based on the mean measured concentrations.

 

Marine water:

In a 28-day flow-through toxicity key study (Wildlife International, 394391, 2013) with saltwater mysids (Americamysis bahia) the NOEC, based on reproduction, for the test substance was determined to be 4.0 ng/L, based on mean measured concentrations.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

181 ng/L

Marine water invertebrates

Marine water invertebrates

Effect concentration:

4 ng/L

Additional information

Freshwater:

In a 7-day flow-through toxicity laboratory study (ABC Laboratories, 704056, 2014) according to ISO 20665 (2008), neonates (< 24-h old) of water fleas, Ceriodaphnia dubia, were exposed to nominal concentrations of 50, 100, 200, 400, and 800 ng/L, corresponding to mean measured concentration 37.8, 92.8, 181, 403, and 755 ng/L, respectively. The control and each treatment consist of 10 100-mL glass beaker with one daphnid each, containing synthetic freshwater at a depth of approx. 3.2 cm. Observations were made daily on the number of surviving adults, occurrence of abnormalities, and production of neonates. The biological results are based on the mean measured concentrations. After 7 days the NOEC for the number of live young per surviving adult were 181 ng/L, based on the mean measured concentrations.

In addition in a 21-day semi-static chronic toxicity laboratory study (BASF SE, 394389, 2014) according to OECD 211 (2012), neonates (> 2 but< 24-h old) of water fleas, Daphnia magna, were exposed to nominal concentrations of 60, 90, 135, 203 and 305 ng/L, corresponding to time weighted mean concentrations of 62, 83, 120, 184 and 286 ng/L, respectively. The NOEC of the test substance for reproduction and body weight was 203 ng/L, based on nominal concentration and 184 ng/L, based on time weighted mean concentration. 

 

Moreover in a 10-day semi-static chronic toxicity laboratory supporting study (BASF SE, 704059, 2014) with Moina macrocopa the NOEC of the test substance for reproduction was 400 ng/L, based on nominal concentration and 448 ng/L, based on time weighted mean concentration.

 

Marine water:

In a 28-day flow-through chronic toxicity laboratory key study (Wildlife International, 394391, 2013) according to EPA OPPTS 850.1350 ((Mysid Chronic Toxicity Test), 1996), juvenile (< 24 h) saltwater mysids (Americamysis bahia) were exposed to nominal concentrations of 2.5, 5.0, 10, 20 and 40 ng/L, corresponding to mean measured concentrations of 2.0, 4.0, 7.1, 14 and 30 ng/L, respectively. Additionally a dilution water control and a solvent (dimethylformamide) control were set up. The animals tested in in groups of 15 mysids in each test chamber containing sand-filtered and UV-sterilized seawater with four replicates per concentration. On Day 14 of the test, male and female adults of the parental generation (G1) were paired in each treatment and control group, with a maximum of five reproductive pairs per replicate. Observations for survival and signs of toxicity were conducted daily throughout the test for the G1 and also produced young (G2) mysids and the growth (total body lengths and dry weights) of all surviving G1 mysids as well as the number of survived animals were determined at test termination. Reproduction of the paired mysids was monitored also on Day 28. The biological results are based on mean measured concentrations. After 14 days of exposure the mean survival rates of juvenile mysids in the test item treatment groups ranged from 95.0% to 98.3 %. At test termination, mean survival rates of adult mysids were 83.0, 85.5, 88.7, 80.8, 97.2, 82.5, 86.7 and 58.3 % in negative control, solvent control, 2.0, 4.0, 7.1, 14 and 30 ng/L treatments, respectively. Statistically significant decrease in survival in comparison to the pooled control were observed only in concentration of 30 ng/L. The mean number of offspring per reproductive day in negative control, solvent control, 2.0, 4.0, 7.1, 14 and 30 ng/L treatments were 0.564, 0.970, 0.766, 0.718, 0.381, 0.225 and 0.043, respectively. Statistically significant decrease were observed at concentrations of 14 and 30 ng/L. However despite no statistical significance, there is a notable decrease in the 7.1 ng/L treatment group and, therefore, a treatment-related effect cannot be precluded for this test level. Reproduction was the most sensitive biological endpoint measured. At test end, the mean total length and dry weight of male adult mysids in the test item treatment groups ranged from 7.74 to 7.90 mm and from 0.74 to 0.82 mg, respectively. The mean total length and dry weight of female adult mysids at test termination ranged 7.99 to 8.20 mm and 0.98 to 1.07 mg, respectively. There were no statistically significant decreases in body length and dry weight of adults in comparison to the pooled control. No results with positive control were determined. After 28 days the NOEC was determined to be 4 ng/L.