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No growth inhibition study on aquatic plants could be performed due to the physico-chemical properties of the susbtance.

The substance is highly volatile (84 kPa) and highly insoluble in water. According to Annex VII, section 9.1.2., column 2 of Regulation No. 1907/2006, the study of growth inhibition of aquatic plant study does not need to be conducted as the substance is highly insoluble.

One key study (Migchielsen, 2017) is available to assess the short-term toxicity of the registered substance to aquatic invertebrates, Daphnia magna. This study was performed according to OECD Guideline 202, under semi-static conditions for 48 hours.

A limit test at 150 mg/L has been performed. The initial measured concentration of test substance was only 1.95 mg/L. All the daphnids exposed to this concentration of test substance were immobile after 24 hours of exposure, whereas all the daphnids exposed to the control were still mobile. Microscopic observation revealed that the immobilised daphnids had deceased. The experiment was consequently stopped and effect concentrations could not be determined. In conclusion, due to the technical difficulties it was not possible to generate reliable results to calculate the effect concentration. It was however concluded that it is highly likely that the 24h-EC50 is below 1.95 mg/L according to the values of the measured concentrations over the first 24 hours of the test, that caused 100% immobilisation of daphnids.

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