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Short term toxicity to aquatic invertebrate

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the short term toxicity on aquatic invertebrate predicted for Cinnamyl formate (CAS: 104-65-4). Effect concentration i.e EC50 value estimated to be 148.28 mg/l for Daphnia magna for 48 hrs duration. It can be concluded that the Cinnamyl formate (CAS: 104-65-4) is likely to be not toxic to aquatic invertebrates, hence it can be considered to be “not classified” as per the CLP classification criteria for aquatic environment.

Toxicity to aquatic algae and cyanobacteria

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the short term toxicity on aquatic algae predicted for (2E)-3-phenylprop-2-en-1-yl formate (CAS: 104-65-4). Effect concentration i.e EC50 value estimated to be 228.17 mg/l for algae for 72 hrs duration. It can be concluded that the (2E)-3-phenylprop-2-en-1-yl formate (CAS: 104-65-4) IS likely to be not toxic to aquatic algae, hence it can be considered to benot classifiedas per the CLP classification criteria for aquatic environment.

Additional information

Short term toxicity to aquatic invertebrate

Following studies include the predicted data and experimental study for the target chemical and structurally similar read across to conclude the toxicity extent of Cinnamyl formate (CAS: 104-65-4) towards aquatic invertebrate is summarized as follows:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the short term toxicity on aquatic invertebrate predicted for Cinnamyl formate (CAS: 104-65-4). Effect concentration i.e EC50 value estimated to be 148.28 mg/l for Daphnia magna for 48 hrs duration. It can be concluded that the Cinnamyl formate (CAS: 104-65-4) is likely to be not toxic to aquatic invertebrates, hence it can be considered to be “not classified” as per the CLP classification criteria for aquatic environment.

The predicted results for target assisted by experimental data of read across2-phenylethyl propanoate; Phenethyl propionate (CAS:122 -70 -3) from the publication Pakistan journal of zoologyT 2007, suggests thatthe naturally occurring compounds including target chemical 2-phenylethyl propanoate have been tested for molluscicidal activity against the fresh water snail, Biomphalaria alexandrina.The recommended World Health Organization bioassay procedure (WHO, 1965), was used for the evaluation of 2-phenylethyl propanoate on B. alexandrina.

For experimentBiomphalaria alexandrina species8mm ± 2mm diameter were collected from fresh water ponds at Kafr El-Dwar, Behera Governorate, Egypt and were maintained in glass aquaria oxygenated for one hour daily, one week before testing. The tests were performed in 150 ml glass beakers with ten adult healthy snails per assay. Each chemical was mixed with Tween 20, to ensure complete solubility of the chemical in water. The mixture was added to the glass beaker filled up to 100 ml of the dechlorinated water to give the desired concentration (w/v) up to 400 μg/ml for chemical.

The snails were exposed for 24 h to different concentrations of compounds or their mixture with PB and then transferred to dechlorinated water for another 48 h. Percentage kill were recorded after 72 h. Percentage mortality was corrected using Abbott's formula (Abbott, 1925). Toxicity parameters for each treatment were computed according to the probit-analysis method by Finney (1971).

According to experimental result the lethal concentration (LC50) value of phenethyl propionate in aquatic invertebrate [Biomphalaria alexandrina ] in a 72 hr study on the basis of mortality effect was found to be 296.27 mg/L. It can be concluded fom the value that the phenethyl propionate is not toxic to the aquatic invertebrate and can be considered as "not classified" as per the classifiecation criteria.

Further it is supported by the another experimental study of structurally similar read acrossPropyl acetate (CAS:109-60-4) from publication Zeitschrift für Wasser und Abwasserforschung 1982 also suggests that the Short-term toxicity to aquatic invertebrates test was carried out for Propyl acetate to study the effects on aquatic environment.. Behavior Equilibriumeffects was measured during the test.Effective concentration EC50 to 50% ofDaphnia magna for Propyl acetate is 318 mg/LIt can be concluded from the value that the Propyl acetate is not toxic to the aquatic invertebrate and can be considered as “not classified per the classification criteria for aquatic environment.

Thus based on the effect concentrations which is in the range 148.28 mg/l to318 mg/lgive the conclusion that test substanceCinnamyl formate (CAS: 104-65-4)is likely to be not toxic to aquatic invertebrate at environmentally relevant concentrations and applying weight of evidence approach it can be considered to be “not classified” as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria

Following studies include the predicted data and experimental study for the target chemical and structurally similar read across to conclude the toxicity extent of Cinnamyl formate (CAS: 104-65-4) towards aquatic algae is summarized as follows:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the short term toxicity on aquatic algae predicted for (2E)-3-phenylprop-2-en-1-yl formate (CAS: 104-65-4). Effect concentration i.e EC50 value estimated to be 228.17 mg/l for Daphnia magna for 72 hrs duration. It can be concluded that the (2E)-3-phenylprop-2-en-1-yl formate (CAS: 104-65-4) IS likely to be not toxic to aquatic algae, hence it can be considered to benot classifiedas per the CLP classification criteria for aquatic environment.

The predicted results for target assisted by experimental data of read across2-phenylethyl propanoate; Propyl acetate (CAS:109-60-4)

from the publication Environmental Science and Pollution Research 2006, suggests that the No-observable-effect-concentration NOEC to Scenedesmus subspicatus for Propyl acetate is 250 mg/L. It can be concluded from the value that the Propyl acetate is not toxic to the aquatic algae and can be considered as “not classified” as per the classification criteria for aquatic environment.

 

Further it is supported by the another experimental study of structurally similar read acrossMaleic anhydride (CAS: 108-31-6) froom secondary source also suggests that theeffective concentration EC50 to 50% of Haematococcus pluvial in 4h is 190 mg/L. inhibition of O2 production was measured during the test. It can be concluded from the value that the Maleic anhydride is not toxic to the aquatic algae and can be considered as “not classified” as per the classification criteria for aquatic environment.

Thus based on the effect concentrations which is in the range 190 mg/l to 228.17 mg/l give the conclusion that test substance Cinnamyl formate (CAS: 104-65-4) was likely to be not toxic to aquatic algae at environmentally relevant concentrations and applying weight of evidence approach it can be considered to benot classifiedas per the CLP classification criteria.