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EC number: 233-823-0 | CAS number: 10377-52-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- publication
- Title:
- Primary mutagenicity screening of food additives currently used in Japan
- Author:
- M. Ishidate, JR et al.
- Year:
- 1 984
- Bibliographic source:
- Fd Chem. Toxic. Vol, 22, No. 8, pp. 623-636, 1984
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 473 (In Vitro Mammalian Chromosome Aberration Test)
- GLP compliance:
- no
- Type of assay:
- other: in vitro mammalian chromosome aberration test
Test material
- Reference substance name:
- Disodium hydrogenorthophosphate
- EC Number:
- 231-448-7
- EC Name:
- Disodium hydrogenorthophosphate
- Cas Number:
- 7558-79-4
- Molecular formula:
- H3O4P.2Na
- IUPAC Name:
- disodium hydrogen phosphate
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- mammalian cell line, other: Chinese hamster lung fibroblast cell line (CHL)
- Details on mammalian cell type (if applicable):
- - Type and identity of media: Minimum Essential Medium (MEM;GIBCO) supplemented with 10 % calf serum
- Properly maintained: yes - Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- without
- Test concentrations with justification for top dose:
- Three concentrations up to a maximum of 1 mg/mL (the highest non cytotoxic dose used in this experiment)
- Vehicle / solvent:
- - Vehicle used: physiol. saline
- Justification for choice of vehicle: Solubility of the test substance was adequat in this vehicle.
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- no
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium
DURATION
- Exposure duration: 24 and 48 hours
SPINDLE INHIBITOR (cytogenetic assays): Colcemid (0.2 µg/mL) was added to the cultures two hours befor harvesting.
STAIN (for cytogenetic assays): with Giemsa solution (1.5 %; at pH 6.8)
NUMBER OF REPLICATIONS: 1
NUMBER OF CELLS EVALUATED: 100 well spread metaphases were analysed.
OTHER EXAMINATIONS:
- Determination of polyploidy: Yes - Evaluation criteria:
- The results were considered to be negative if the incidence was less than 4.9 %, equivocal if it was between 5.0 and 9.9 %, and positive if it was more than 10.0 %.
Results and discussion
Test results
- Key result
- Species / strain:
- Chinese hamster lung fibroblasts (V79)
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not determined
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- not examined
Applicant's summary and conclusion
- Conclusions:
- Under the described test conditions, sodium phosphate monobasic is regarded as not clastogenic.
- Executive summary:
In a chromosome aberration test equivalent or similar to OECD guideline 473, a Chinese hamster fibroblast cell was exposed to sodium phosphate monobasic (three different doses for 24 h and 48 h). The maximum dose of each sample was choosen via a preliminary test (dose needed for 50 % cell-growth inhibition). Untreated cells and solvent-treated cells were used as negative controls when the incidence of aberrations was less than 3 %. The results were considered as negative if the incidence was <= 4.9 % and positive if it was > 10 %. The test results for sodium phosphate monobasic were negative.
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