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Toxicological information

Basic toxicokinetics

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Administrative data

Endpoint:
basic toxicokinetics in vivo
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable publication which meets basic scientific principles For justification of read across please refer to section 13.

Data source

Reference
Reference Type:
publication
Title:
The Appearance of some congeners of alcoholic beverages and their metabolites in blood
Author:
Greenberg LA
Year:
1970
Bibliographic source:
Q.J. Stud. Alcohol, Suppl 5, 20-25

Materials and methods

Objective of study:
toxicokinetics
Test guideline
Qualifier:
no guideline available
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): isoamyl alcohol
Radiolabelling:
no

Test animals

Species:
rat
Strain:
other: albino
Sex:
not specified
Details on test animals and environmental conditions:
TEST ANIMALS
- Age at study initiation: adult

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
other: 20% ethanol or water
Duration and frequency of treatment / exposure:
4 equally divided doses (25 ml/kg bw) at 15 min intervals
Doses / concentrations
Remarks:
Doses / Concentrations:
600 mg/l
No. of animals per sex per dose:
10
Control animals:
no
Details on dosing and sampling:
PHARMACOKINETIC STUDY (Absorption, distribution, excretion)
- Tissues and body fluids sampled: blood
- Time and frequency of sampling: at 2 h intervals
- Method type(s) for identification: GC-MS

Results and discussion

Metabolite characterisation studies

Metabolites identified:
yes
Details on metabolites:
Isovaleraldehyde has been identified as intermediary metabolite of 3-methylbutan-1-ol .

Any other information on results incl. tables

Results:

After administration of isoamyl alcohol with ethanol:

h after administration Etanol (mg/ml Isoamyl alcohol (peak units) Isovaleraldehyde (peak units)
2 4.8 16 2
4 3.7 13.5 2.5
6 3 7 3.5
8 2.1 3.5 3
10 2.3 1.5 0
12 0.7 0 0

With water as vehicle, blood isoamyl alcohol was detected for 2 h after dosing. 3 -Methylbutyraldehyde was not seen.

With 20% ethanol in water, isoamyl alcohol was seen for 10 h after dosing & 3-methylbutyraldehyde for 8h.

Applicant's summary and conclusion