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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Data is from publication.

Data source

Reference
Reference Type:
publication
Title:
Mutagenicity of quinolines in Salmonella typhimurium TA100 A QSAR study based on hydrophobicity and molecular orbital determinants
Author:
Asim Kumar Debnath , Rosa L. Lopez de Compadre and Corwin Hansch
Year:
1992
Bibliographic source:
Mutation Research, 280 (1992) 55-65

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: As mention below
Principles of method if other than guideline:
To evaluate the mutagenic potential of 3-Bromoquinoline in Salmonella typhimurium strains TA 98 and TA 100 by Ames test.
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid
Details on test material:
- Name of test material : 3-Bromoquinoline
- Molecular formula : C9H6BrN
- Molecular weight : 208.057 g/mol
- Smiles notation : c12c(ncc(c1)Br)cccc2
- InChl : 1S/C9H6BrN/c10-8-5-7-3-1-2-4-9(7)11-6-8/h1-6H
- Substance type: Organic
- Physical state: Liquid
Specific details on test material used for the study:
Details on test material
- Name of test material (as cited in study report):
- Molecular formula: C9H6BrN
- Molecular weight:
- Substance type: 208.057g/mol
- Physical state: Organic
Purity;> 99% using HPLC.

Method

Target gene:
Histidine
Species / strain
Species / strain:
other: TA 98 and TA 100
Details on mammalian cell lines (if applicable):
Not applicable
Additional strain characteristics:
not specified
Cytokinesis block (if used):
not specified
Metabolic activation:
with and without
Metabolic activation system:
S9 microsomal fraction
Test concentrations with justification for top dose:
0,6.7,10,33,67,100,333,667 and 1000 µg/plate
Vehicle:
Vehicle
- Vehicle(s)/solvent(s) used: DMSO ,50µl
Controls
Negative controls:
not specified
Solvent controls:
yes
Remarks:
DMSO
True negative controls:
not specified
Positive controls:
yes
Positive control substance:
other: (+ S9) 2-aminoanthracene ; TA98 and TA100 (-S9) 2-nitrofluorene ; TA98 (-S9)sodium azide;TA 100
Details on test system and conditions:
Details on test system and conditions
METHOD OF APPLICATION: in medium; in agar (plate incorporation
DURATION
- Preincubation period: 20 min
- Exposure duration: 48 hour

NUMBER OF REPLICATIONS: Triplicates

Other: Plates not counted immediately were stored at 4°C. An automated colony counter was used for counting except when there was interference due to precipitation of test article and then manual counting was used.
Rationale for test conditions:
Not specified
Evaluation criteria:
The revertants/ nmole were calculated by converting dose in Kg to nmole and taking the slope of the linear regression line of the dose-response curve. The logarithms of the slopes (revertants/ nmole) were used as activity parameters
Statistics:
Mean± Standard deviation was observed.

Results and discussion

Test results
Species / strain:
other: TA 98 and TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity:
not specified
Vehicle controls valid:
yes
Negative controls valid:
not specified
Positive controls valid:
yes
Remarks on result:
other: No mutagenic effect were observed.

Any other information on results incl. tables

Sr no

 

Dose

 

Revertants/plate

(mean ±standard

Deviation100 (+ S9)

1

Control

 

 

 

 

DMSO

50µl

153± 3

2

 Test substance name

(µg/plate)

 

 

3 -Bromoquinoline

 

 

 

6.7

152± 17

 

10

160±9

 

33

125±7

 

67

157± 6

 

100

121±15

 

333

133±16

 

667

34±41

 

1000

0±0

 

Applicant's summary and conclusion

Conclusions:
3-Bromoquinoline (5332-24-1) was evaluated for its mutagenic potential in Salmonella typhimurium strains TA 98 and TA 100 by Ames test. The test result was considered to be negative with and without S9.
Executive summary:

3-Bromoquinoline was assessed for its possible mutagenic potential. For this purpose AMES test was performed  in Salmonella typhimurium strains TA 98 and TA 100. The test material was exposed at the concentration of 0, 6.7,10,33,67,100,333,667 and 1000 µg/plate in the presence and absence of S9. No mutagenic effects were observed. Only the data on TA100 with metabolic activation are reported here (Table) as the test substances tested on TA98 with and without S9 activation and on TA100without S9 activation were inactive. Therefore 3-Bromoquinoline was considered to be non mutagenic in presence and absence of S9.Hence the substance cannot be classified as gene mutant in vitro.