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Toxicological information

Skin sensitisation

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Administrative data

Endpoint:
skin sensitisation: in vivo (non-LLNA)
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
data from handbook or collection of data

Data source

Reference
Reference Type:
review article or handbook
Title:
determination of Skin sensitization for CAS no: 100-00-5
Author:
HSDB
Year:
2017
Bibliographic source:
U.S. National Library of Medicine National Institutes of Health, Health & Human Services; 2017.

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
The Mouse ear swelling test was performed on five mice to assess the skin sensitization potential of chemical 1-Chloro-4-nitrobenzene (CAS no: 100-00-5).
GLP compliance:
not specified
Type of study:
mouse ear swelling test
Justification for non-LLNA method:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- IUPAC Name: 1-Chloro-4-nitrobenzene
- Common Name: 4-Chloronitrobenzene
- Mol. formula: C6H4ClNO2
- Molecular Weight: 157.556 g/mole
- Smiles: c1([N+](=O)[O-])ccc(Cl)cc1
- InChI: 1S/C6H4ClNO2/c7-5-1-3-6(4-2-5)8(9)10/h1-4H

Specific details on test material used for the study:
- IUPAC Name: 1-Chloro-4-nitrobenzene
- Common Name: 4-Chloronitrobenzene
- Mol. formula: C6H4ClNO2
- Molecular Weight: 157.556 g/mole
- Smiles: c1([N+](=O)[O-])ccc(Cl)cc1
- InChI: 1S/C6H4ClNO2/c7-5-1-3-6(4-2-5)8(9)10/h1-4H

In vivo test system

Test animals

Species:
mouse
Strain:
not specified
Sex:
not specified
Details on test animals and environmental conditions:
No data available

Study design: in vivo (non-LLNA)

Induction
Route:
other: epicutaneous
Vehicle:
other: acetone
Concentration / amount:
0.2 mL of a 0.05 M solution
Day(s)/duration:
No data available
Adequacy of induction:
not specified
Challenge
No.:
#1
Route:
other: epicutaneous
Vehicle:
other: Acetone
Concentration / amount:
20 uL of 0.016 M solution
Day(s)/duration:
72 hours
Adequacy of challenge:
highest non-irritant concentration
No. of animals per dose:
5
Details on study design:
MAIN STUDY
A. INDUCTION EXPOSURE
- No. of exposures: 1
- Exposure period: no data
- Test groups: 5 mice
- Control group: no data
- Site: shaved back of each mice
- Frequency of applications: no data
- Duration: no data
- Concentrations: 0.2 mL of a 0.05 M solution

B. CHALLENGE EXPOSURE
- No. of exposures: 1
- Day(s) of challenge: on day 5
- Exposure period: 24 hours
- Test groups: 5 mice
- Control group: o data
- Site: the surface of one ear of each
mouse
- Concentrations: 20 uL of 0.016 M solution
- Evaluation (hr after challenge): 24 , 48 and 72 hours
Challenge controls:
Controls were used but detail information was not mentioned.

Results and discussion

In vivo (non-LLNA)

Results
Reading:
1st reading
Hours after challenge:
72
Group:
test group
Dose level:
20 uL of 0.016 M solution
No. with + reactions:
0
Total no. in group:
5
Clinical observations:
No skin sensitization was observed.
Remarks on result:
no indication of skin sensitisation

In vivo (LLNA)

Cellular proliferation data / Observations:
No skin sensitization was observed.

Applicant's summary and conclusion

Interpretation of results:
other: Not sensitizing
Conclusions:
The test material 1-Chloro-4-nitrobenzene (CAS no: 100-00-5) was considered to be not sensitizing to the skin of mice.
Executive summary:

The Mouse ear swelling test was performed on five mice to assess the skin sensitization potential of chemical 1-Chloro-4-nitrobenzene (CAS no: 100-00-5).

 

The backs of 5 mice were shaved with electric clippers before induction was performed by applying 0.2 mL of a 0.05 M solution of the compound in acetone on day 1. Challenge application was performed with 20 uL of non-irritant 0.016 M solution to the surface of one ear of each mouse on day 5.

 

Reactions were read after a further 24, 48 and 72 hours by comparison of the thickness of the ears between test mice and controls.

 

Since no known effects were observed, the chemical1-Chloro-4-nitrobenzene (CAS no: 100-00-5) was considered to be not sensitizing to the skin of mice.