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Description of key information

Short term toxicity to aquatic invertebrates

Using the OECD QSAR toolbox version 3.3 (2017) with log kow as the primary descriptor and considering the five closest read across substances, the short term toxicity on aquatic invertebrates was predicted for target substance 3-Bromoquinoline (CAS no. 5332 -24 -1). EC50 value was estimated to be 403 mg/l for Daphnia magna for 48 h duration. Based on this value it can be concluded that the substance 3-Bromoquinoline is considered to be non-toxic to aquatic environment and can be considered to be not classified as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria

Using the OECD QSAR toolbox version 3.3 (2017) with log kow as the primary descriptor and considering the five closest read across substances, the short term toxicity on aquatic algae and cyanobacteria was predicted for target substance 3-Bromoquinoline (CAS no. 5332 -24 -1). EC50 value was estimated to be 129 mg/l for Pseudokirchneriella subcapitata for 72 h duration. Based on this value it can be concluded that the substance 3-Bromoquinoline is considered to be non-toxic to aquatic environment and can be considered to be not classified as per the CLP classification criteria.

Additional information

Short term toxicity to aquatic invertebrates

Predicted data for the target chemical 3-Bromoquinoline (CAS No. 5332-24-1) and various supporting weight of evidence studies for its closest read across substances with logKow as the primary descriptorwere reviewed to summarize the following information:

 

Short term toxicity on aquatic invertebrate of target chemical3-Bromoquinoline(CAS No. 5332-24-1) is predicted using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances (2017). On the basis of effects observed in a static freshwater system, the effect concentration EC50 value for the substance is estimated to be 402 mg/l for Daphnia magna for 48 hr duration.Based on this value it can be concluded that the substance 3-Bromoquinoline is considered to be non-toxic to aquatic environment and can be considered to be not classified as per the CLP classification criteria.

 

In a weight of evidencestudy from authoritative database (J-CHECK, 2017) of the read across chemical 2-hydroxy benzoic acid (CAS no. 69-72-7), short term toxicity to Daphnia magna study was carried out for 48 hrs. The study was performed according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). The study was based on the effects of the read across compound 2 -hydroxy benzoic acid (CAS no. 69 -72 -7) on Daphnia magna in a static fresh water system. Test chemical concentration used for the study were 5.29, 9.53, 17.1, 30.9, 55.6 and 100 mg/l (geometric ratio 1.8) (nominal concentrations). No. of organisms used were 20 per concentrations, respectively. Based on effect on immobilization of the test organism Daphnia magna, the 48 hr EC50 value was determined to be 76.8 mg/l, respectively. Thus, based on the EC50 value, it can be concluded that the substance 2 -hydroxy benzoic acid can be considered as toxic to aquatic organisms. Since the chemical is readily biodegradable in nature, read across chemical 2 -hydroxy benzoic acid can be considered as non-toxic to aquatic organisms and thus can be considered to be not classified as per the CLP classification criteria.

 

In an another study from peer reviewed journal (RENATE KUHN; et. al, 1989) for the read across chemical 4-hydroxy benzoic acid (CAS no. 99-96-7),short term toxicity to Daphnia magna study was carried out for 48 hrs. Acute Daphnia test was performed according to DIN 38412, Part II. The study was based on the effects of the read across compound 4-hydroxy benzoic acid (CAS no. 99-96-7) on Daphnia magna in a static fresh water system at a temperature of 20°C and pH of 8.0±0.2, respectively. Test organism used for the study was 6-24 hr old and no feeding to the organism Daphnia magna was done during the test period. Exact concentration of test chemical used for the study was not known, but the concentration steps of the test solution were selected so as to give 3-4 EC values in a range between EC0 and EC100, of which at least one value was below and one above EC50.The test vessels were two 50 ml beakers and for volatile or strongly smelling substances, two 50 ml bottles with ground-glass stoppers each with 20 ml useful capacity. Two parallel preparations were made for each concentration step. Loading amounted to one test animal per 2 ml test medium as ten 6-24 h old daphnids were placed in each test and control vessel, i.e: 20 animals per concentration step. After a test period of 24 h and again after 48 h, the no. of animals in the control and test solutions that could still swim were counted. After 48 h it was also possible to measure the pH value in order to determine whether the hydrogen ion concentrations were in an acceptable range for the daphnids or whether the result had to be considered as having been influenced by a change in pH during the course of the test. Furthermore, the oxygen content was determined at the end of the test period in order to ensure that it had not fallen below a minimum oxygen concentration of 2 mg/l. The test was considered as valid when fewer than 10% of the animals in the control solutions were unable to swim, when the pH value was not below 7.0 and the O2 value was not below 4.0 m/l. Based on effect on mobility of the test organism Daphnia magna, the 24 hr EC0, EC50 and EC100 value was determined to be 181, 104 and 286 mg/l, respectively and 48 hr EC0, EC50 and EC100 value was determined to be 173, 19 and 204 mg/l, respectively. Thus, based on the 24 and 48 h EC50 value, it can be concluded that the substance 4-hydroxy benzoic acid can be considered as non-toxic to aquatic organisms and thus can be considered to be not classified as per the CLP classification criteria.

 

For the same read across chemical 4-hydroxy benzoic acid (CAS no. 99-96-7), short term toxicity to Daphnia sp. study was carried out for 48 hrs. The study was performed according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). The study was based on the effects of the read across compound 4-hydroxy benzoic acid (CAS no. 99-96-7) on Daphnia sp. in a static fresh water system. Based on effect on immobilization of the test organism Daphnia sp., the 48 hr EC50 value was determined to be 140 mg/l. Thus, based on the EC50 value, it can be concluded that the substance 4-hydroxy benzoic acid can be considered as non-toxic to aquatic organisms and thus can be considered to be not classified as per the CLP classification criteria.

 

Thus, based on the overall reported results for target chemical 3-Bromoquinoline(OECD QSAR toolbox version 3.3, 2017) and for its read across substance (fromauthoritative database J-CHECK and peer reviewed journal),it can be concluded that the test substance3-Bromoquinolinecan be considered as non-toxic to aquatic environment and can be considered to be notclassified as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria

Predicted data for the target chemical 3-Bromoquinoline (CAS No. 5332-24-1) and various supporting weight of evidence studies for its closest read across substances with logKow as the primary descriptorwere reviewed for toxicity to aquatic algae and cyanobacteria endpoint to summarize the following information:

 

Short term toxicity on aquatic algae and cyanobacteria of target chemical3-Bromoquinoline (CAS No. 5332-24-1) is predicted using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances (2017).On the basis of effects observed in a static freshwater system, the effect concentration EC50 value for the substance is estimated to be 129 mg/l for Pseudokirchneriella subcapitata for 72 duration. Based on this value it can be concluded that the substance 3-Bromoquinoline is considered to be non-toxic to aquatic environment and can be considered to be not classified as per the CLP classification criteria.

 

In a supporting weight of evidence study from authoritative database (J-CHECK, 2017) of the read across chemical Benzoic acid, 4-hydroxy-. methyl ester (CAS no. 99-76-3), short term toxicity to green algae study was carried out for 72 hrs. The study was performed according to OECD Guideline 201 (Alga, Growth Inhibition Test).The study was based on the effects of the read across compound Benzoic acid, 4-hydroxy-. methyl ester on green algae in a static fresh water system. Based on effect on growth rate of the test organism green algae, the 72 hr EC50 and NOEC value was determined to be 56 and 17 mg/l, respectively and on the basis of AUG, the 72 hr EC50 and NOEC value was determined to be 30 and 17 mg/l, respectively. Thus, based on the EC50 value, it can be concluded that the substance Benzoic acid, 4-hydroxy-. methyl ester can be considered as toxic to aquatic organisms. Since the chemical is readily biodegradable in nature, chemical Benzoic acid, 4-hydroxy-. methyl ester can be considered as non-toxic to aquatic organisms and thus can be considered to be not classified as per the CLP classification criteria.

 

For the read across chemical 1-Hydroxy-3-amino-benzene (CAS no. 591-27-5), short term toxicity to green algae study was carried out for 72 hrs. The study was based on the effects of the read across compound 1-Hydroxy-3-amino-benzene on green algae in a static fresh water system. Based on effect on growth rate of the test organism green algae, the 72 hr EC50 and NOEC value was determined to be 160 and 25 mg/l, respectively and on the basis of biomass and areas under the growth curves, the 72 hr EC50 and NOEC value was determined to be 62 and 25 mg/l, respectively. Thus, based on the EC50 value (160 mg/l), it can be concluded that the substance 1-Hydroxy-3-amino-benzene can be considered as non-toxic to aquatic organisms and thus can be considered to be not classified as per the CLP classification criteria.

 

Thus, based on the overall reported results for target chemical3-Bromoquinoline(OECD QSAR toolbox version 3.3, 2017) and for its read across substance (from authoritative database J-CHECK), it can be concluded that the test substance 3-Bromoquinoline can be considered as non-toxic to aquatic environment and can be considered to be notclassified as per the CLP classification criteria.

On the basis of above mentioned available information for short term toxicity to aquatic invertebrates and toxicity to aquatic algae and cyanobacteria, test substance 3-Bromoquinoline can be considered as non-toxic to aquatic environment and can be considered to be not classified as per the CLP classification criteria.