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Toxicological information

Skin sensitisation

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Administrative data

Endpoint:
skin sensitisation: in vivo (non-LLNA)
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data from peer reviewed journal

Data source

Reference
Reference Type:
publication
Title:
Allergic contact dermatitis from the test chemical
Author:
Pickerlng, F. C et.al
Year:
1982
Bibliographic source:
Contact Dermatitis, 1982

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: As mentioned below
Principles of method if other than guideline:
Human maximization test was perfiormed to determine the sensitization potential of the test chemical
GLP compliance:
not specified
Type of study:
patch test
Justification for non-LLNA method:
No data available

Test material

Constituent 1
Chemical structure
Reference substance name:
4,7-dichloroquinoline
EC Number:
201-714-7
EC Name:
4,7-dichloroquinoline
Cas Number:
86-98-6
Molecular formula:
C9H5Cl2N
IUPAC Name:
4,7-dichloroquinoline
Test material form:
solid
Details on test material:
- Name of test material: 4,7-Dichloroquinoline
- IUPAC name: 4,7-Dichloroquinoline
- Molecular formula: C9H5Cl2N
- Molecular weight: 198.051 g/mole
- Smiles : c12c(cc(Cl)cc2)nccc1Cl
- Inchl: 1S/C9H5Cl2N/c10-6-1-2-7-8(11)3-4-12-9(7)5-6/h1-5H
- Substance type: Organic
- Physical state: Solid

In vivo test system

Test animals

Species:
other: Human
Strain:
not specified
Sex:
not specified
Details on test animals and environmental conditions:
No data available

Study design: in vivo (non-LLNA)

Induction
Route:
epicutaneous, occlusive
Vehicle:
petrolatum
Concentration / amount:
5% in petrolatum
Adequacy of induction:
not specified
Challenge
No.:
#1
Route:
epicutaneous, occlusive
Vehicle:
petrolatum
Concentration / amount:
5% in petrolatum
Adequacy of challenge:
not specified
No. of animals per dose:
6 workers
Details on study design:
RANGE FINDING TESTS:

MAIN STUDY
A. INDUCTION EXPOSURE
- Test groups: 6 workers
- Concentrations: 5% in petrolatum

B. CHALLENGE EXPOSURE
- Test groups: 6
- Concentrations: 5% in petrolatum
- Evaluation (hr after challenge): 48 and 96 hours
Challenge controls:
No data available
Positive control substance(s):
not specified

Results and discussion

Positive control results:
No data available

In vivo (non-LLNA)

Results
Reading:
1st reading
Hours after challenge:
48
Group:
test chemical
Dose level:
5%
No. with + reactions:
2
Total no. in group:
4
Clinical observations:
reappearance of the original rash in case 1 and 2
Remarks on result:
positive indication of skin sensitisation

Any other information on results incl. tables

Results of the patch tests

48hr

96hr

1

++

++

2

++

++

3

+

-

4

-

-

5

Not performed

 

6

Not performed

 

Applicant's summary and conclusion

Interpretation of results:
Category 1 (skin sensitising) based on GHS criteria
Conclusions:
Based on the observations, reactions, it can be considered that the occupational exposure to the test chemical can cause dermatitis to the operators, so the test chemical should be handled with proper care. Hence, it can be considered to be sensitizer to skin.
Executive summary:

The study reports of a series of patch tests performed on process operators operating in a chemical plant using the test chemical. 6 workers developed dermatitis of the face and were employed in jobs involving the charging the test chemical to a hot reaction vessel. The workers were patch tested initially with 50% test chemical in petrolatum. The reactions were read at 72 hours as ++ positive. The patch test was again repeated with 5% test chemical in petrolatum and reactions were observed and scored at 48 and 96 hours. Patch tests on normal controls were negative. Of the 6 workers tested only 4 workers showed positive reactions at 48 as well as at 96 hours. Further investigations indicated that the dermal reactions were only seen in workers charging the test chemical to a hot reaction vessel, but not amongst other workers handling the test chemical. Upon investigation it was observed that the test chemical could sublime at high temperatures, and this property of the chemical could be a possible reason for development of facial dermatitis in workers involved in charging the test chemical. Based on the observations, reactions, it can be considered that the occupational exposure to the test chemical can cause dermatitis to the operators, so the test chemical should be handled with proper care. Hence, it can be considered to be sensitizer to skin.