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Ecotoxicological information

Toxicity to microorganisms

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Reference
Endpoint:
toxicity to microorganisms, other
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
Experimental data of test chemicals
Justification for type of information:
data from handbook or collection of data
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Qualifier:
according to guideline
Guideline:
other: Refer below principle
Principles of method if other than guideline:
WoE was prepared for the determination of effect of test chemical on the growth rate of microorganisms.
GLP compliance:
not specified
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
other: WoE - 2: Tetrahymena thermophila, WoE - 3: Photobacterium phosphoreum
Details on inoculum:
WoE - 2:
Species Latin Name: Tetrahymena thermophila
Species Common Name: Ciliate Protozoa
Organism Life Stage: Stationary growth phase
Organism Characteristics: 10000 CELLS/ML
Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h
Remarks on exposure duration:
15 minutes exposure in WoE 3
Test temperature:
WoE - 2: 32 °C
Reference substance (positive control):
not specified
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
25.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks on result:
other: Effects were observed on population growth rate inhibition
Remarks:
WoE - 2
Duration:
15 min
Dose descriptor:
EC50
Effect conc.:
3.29 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Inhibition of luminescence
Remarks on result:
other: WoE - 3
Validity criteria fulfilled:
not specified
Conclusions:
As the study was conducted on two different organisms, effects were measured on the basis of different parameter. Based on the population growth rate inhibition of microorganisms Tetrahymena thermophila (Ciliate Protozoa), due to the test chemical exposure for 48 hours, the EC50 value was determined to be 25.5 mg/l. Also based on the inhibition of luminescence Photobacterium phosphoreum, due to the test chemical
exposure for 15 minutes, the EC50 value was determined to be 3.29 mg/l. Thus on the basis of above all studies, the EC50 value ranges from 3.29 to 25.5 mg/l.
Executive summary:

Based on the various experimental studies from different sources, studies were reviewed for the determination of effects of test chemical on the growth of microorganisms and the studies are as mention below:

Principle of this study from secondary source was to determine the effect of test chemical on the population growth rate inhibition of microorganisms Tetrahymena thermophila (Ciliate Protozoa). Test conducted under the static system for 48 hours. After the exposure period of 48 hours, effects on the population growth was observed and noted. Based on the population growth rate inhibition of microorganisms Tetrahymena thermophila (Ciliate Protozoa), due to the test chemical exposure for 48 hours, the EC50 value was determined to be 25.5 mg/l.

Above study further supported by the weight of evidence data from secondary sources. Principle of this study was to determine the effect of test chemical on the luminescence of Photobacterium phosphoreum. Test conducted for 15 minutes. Based on the inhibition of luminescence Photobacterium phosphoreum, due to the test chemical exposure for 15 minutes, the EC50 value was determined to be 3.29 mg/l.

As the study was conducted on two different organisms, effects were measured on the basis of different parameter. Based on the population growth rate inhibition of microorganisms Tetrahymena thermophila (Ciliate Protozoa), due to the test chemical exposure for 48 hours, the EC50 value was determined to be 25.5 mg/l. Also based on the inhibition of luminescence Photobacterium phosphoreum, due to the test chemical exposure for 15 minutes, the EC50 value was determined to be 3.29 mg/l. Thus on the basis of above all studies, the EC50 value ranges from 3.29 to 25.5 mg/l.

Description of key information

As the study was conducted on two different organisms, effects were measured on the basis of different parameter. Based on the population growth rate inhibition of microorganisms Tetrahymena thermophila (Ciliate Protozoa), due to the test chemical exposure for 48 hours, the EC50 value was determined to be 25.5 mg/l. Also based on the inhibition of luminescence Photobacterium phosphoreum, due to the test chemical exposure for 15 minutes, the EC50 value was determined to be 3.29 mg/l. Thus on the basis of above all studies, the EC50 value ranges from 3.29 to 25.5 mg/l.

Key value for chemical safety assessment

EC50 for microorganisms:
25.5 mg/L

Additional information

Based on the various experimental studies from different sources, studies were reviewed for the determination of effects of test chemical and structually and functionally similar read across chemicals on the growth of microorganisms and the studies are as mention below:

Principle of this study from secondary source was to determine the effect of test chemical on the population growth rate inhibition of microorganisms Tetrahymena thermophila (Ciliate Protozoa). Test conducted under the static system for 48 hours. After the exposure period of 48 hours, effects on the population growth was observed and noted. Based on the population growth rate inhibition of microorganisms Tetrahymena thermophila (Ciliate Protozoa), due to the test chemical exposure for 48 hours, the EC50 value was determined to be 25.5 mg/l.

Above study further supported by the weight of evidence data from secondary sources. Principle of this study was to determine the effect of test chemical on the luminescence of Photobacterium phosphoreum. Test conducted for 15 minutes. Based on the inhibition of luminescence Photobacterium phosphoreum, due to the test chemical exposure for 15 minutes, the EC50 value was determined to be 3.29 mg/l.

As the study was conducted on two different organisms, effects were measured on the basis of different parameter. Based on the population growth rate inhibition of microorganisms Tetrahymena thermophila (Ciliate Protozoa), due to the test chemical exposure for 48 hours, the EC50 value was determined to be 25.5 mg/l. Also based on the inhibition of luminescence Photobacterium phosphoreum, due to the test chemical exposure for 15 minutes, the EC50 value was determined to be 3.29 mg/l. Thus on the basis of above all studies, the EC50 value ranges from 3.29 to 25.5 mg/l.