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Density

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Reference
Endpoint:
relative density
Type of information:
experimental study
Adequacy of study:
key study
Study period:
15 October - 21 December 2016
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
Study was conducted in accordance with international guidelines and in accordance with GLP. All study validity criteria were met.
Qualifier:
according to
Guideline:
OECD Guideline 109 (Density of Liquids and Solids)
Deviations:
no
Qualifier:
according to
Guideline:
EU Method A.3 (Relative Density)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Type of method:
pycnometer method
Specific details on test material used for the study:
RADIOLABELLING INFORMATION (if applicable)
- Radiochemical purity: n/a
- Specific activity: n/a
- Locations of the label: n/a
- Expiration date of radiochemical substance: n/a

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: ca. 4 ºC in the dark under nitrogen.
- Stability under test conditions: Assumed stable
- Solubility and stability of the test substance in the solvent/vehicle: n/a
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: n/a

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: n/a
- Preliminary purification step (if any): n/a
- Final dilution of a dissolved solid, stock liquid or gel: n/a
- Final preparation of a solid: n/a

FORM AS APPLIED IN THE TEST (if different from that of starting material): n/a

OTHER SPECIFICS: n/a
Key result
Type:
density
Density:
889 kg/m³
Temp.:
20 °C
Key result
Type:
relative density
Density:
0.889 other: dimensionless
Temp.:
20 °C

Table 1       Results of duplicate density determinations

Measurement

Determination

1

2

Mass of pycnometer (g)

17.3494

17.0613

Mass of pycnometer and purified water at 20 ºC (g)

27.5645

27.2371

Mass of pycnometer and test item at 20.0 ºC (g)

26.4442

26.1242

Temperature of test item (ºC)

20.0

Density of water at 20 ºC (kg/m3)

998.20

Density (kg/m3)

888.7

889.0

Relative density

0.889

0.889

Conclusions:
The density of the test item has been determined to be 889 at 20.0 ± 0.5 °C; relative density: 0.889.
Executive summary:

EU Method A.3 (2017) - The determination was carried out by the pycnometer method. Separate aliquots of purified water and test item were equilibrated at 20.0 ± 0.5 °C. The pycnometer was cleaned and dried to constant weight, then filled with equilibrated purified water and re-weighed. The pycnometer was emptied and dried, then filled with the equilibrated test item and reweighed. The procedure was performed in duplicate.

The density (ρ) of the test item was calculated based on the ratio of the mass of test item to the mass of water used to fill the pycnometer using the following equation;

p = (d • (c - a)) / (b - a)

where:

a = mass of pycnometer (g)

b = mass of pycnometer filled with water (g)

c = mass of pycnometer filled with test item (g)

d = density of water at test temperature (kg/m3)

The relative density of solids or liquids is the ratio between the mass of a volume of substance to be examined determined at 20 °C, and the mass of the same volume of water determined at 4 °C. Relative density has no dimensions. The density of water determined at 4 °C is 999.97 kg/m3 (as published by the CRC Handbook of Chemistry and Physics, 94th Edition 2014-2015).

The density of the test item has been determined to be 889 at 20.0 ± 0.5 °C; relative density: 0.889.

Description of key information

Relative Density = 0.889 (20.0 ± 0.5 °C); EU Method A.3; Anon. (2017)

Key value for chemical safety assessment

Relative density at 20C:
0.889

Additional information

In a study conducted in accordance with EU Method A.3, the determination was carried out by the pycnometer method. Separate aliquots of purified water and test item were equilibrated at 20.0 ± 0.5 °C. The pycnometer was cleaned and dried to constant weight, then filled with equilibrated purified water and re-weighed. The pycnometer was emptied and dried, then filled with the equilibrated test item and reweighed. The procedure was performed in duplicate.

The density (ρ) of the test item was calculated based on the ratio of the mass of test item to the mass of water used to fill the pycnometer using the following equation;

p = (d • (c - a)) / (b - a)

where:

a = mass of pycnometer (g)

b = mass of pycnometer filled with water (g)

c = mass of pycnometer filled with test item (g)

d = density of water at test temperature (kg/m3)

The relative density of solids or liquids is the ratio between the mass of a volume of substance to be examined determined at 20 °C, and the mass of the same volume of water determined at 4 °C. Relative density has no dimensions. The density of water determined at 4 °C is 999.97 kg/m3 (as published by the CRC Handbook of Chemistry and Physics, 94th Edition 2014-2015).

The density of the test item has been determined to be 889 at 20.0 ± 0.5 °C; relative density: 0.889.