Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

Currently viewing:

Administrative data

Link to relevant study record(s)

Referenceopen allclose all

Endpoint:
toxicity to soil macroorganisms except arthropods: long-term
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Justification for type of information:
See Attached Justification
Qualifier:
according to guideline
Guideline:
other: REACH Guideance on QSARs
Principles of method if other than guideline:
- Software tool(s) used including version:
See Field 'Attached justification'
- Model(s) used:
See Field 'Attached justification'
- Model description: See Field 'Attached justification'
- Justification of QSAR prediction: see field 'Attached justification'
GLP compliance:
not specified
Specific details on test material used for the study:
HC Block Type Carbon Number Concentration (%w/w)
Mono-Aromatic (MoAr) 7 2.49
8 4.24
9 66.33
10 5.12
Naphthenic Mono-Aromatic (NMAr) 9 19.58
Total 97.76

The constituents that could fit in more than one HC block, or that did not fit in any specific block, were placed in a relevant block that presented the highest toxicity.
Analytical monitoring:
not required
Test organisms (species):
Eisenia fetida
Animal group:
annelids
Study type:
other: QSAR
Total exposure duration:
56 d
Reference substance (positive control):
no
Remarks:
Not Required
Duration:
14 d
Dose descriptor:
other: LL50
Effect conc.:
160 mg/kg soil dw
Nominal / measured:
estimated
Conc. based on:
other: QSAR prediction
Basis for effect:
mortality
Remarks on result:
other: QSAR prediction value
Duration:
56 d
Dose descriptor:
other: EL10
Effect conc.:
30.6 mg/kg soil dw
Nominal / measured:
estimated
Conc. based on:
other: QSAR prediction
Basis for effect:
reproduction
Remarks on result:
other: QSAR prediction value
Validity criteria fulfilled:
yes
Conclusions:
The QSAR predicted 14-d LL50 (mortality) and 56-d EL10 (reproduction) for E. fetida were 160 and 30.6 mg/kg dw respectively.
Executive summary:

The QSAR predicted 14-d LL50 (mortality) and 56-d EL10 (reproduction) of the UVCB substance for E. fetida were 160 and 30.6 mg/kg soil dw respectively. The model used for the calculations was the PETROTOX v4.0 model. PETROTOX is a well-documented and peer reviewed model that is widely used across the chemicals industry. This substance fits within the criteria of the model and there are no reservations about the validity of the model runs. It is expected that this data is reliable with restrictions.

Endpoint:
toxicity to soil macroorganisms except arthropods: long-term
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Justification for type of information:
See Attached Justification
Qualifier:
according to guideline
Guideline:
other: REACH Guideance on QSARs
Principles of method if other than guideline:
- Software tool(s) used including version:
See Field 'Attached justification'
- Model(s) used:
See Field 'Attached justification'
- Model description: See Field 'Attached justification'
- Justification of QSAR prediction: see field 'Attached justification'
GLP compliance:
not specified
Specific details on test material used for the study:
HC Block Type Carbon Number Concentration (%w/w)
Mono-Aromatic (MoAr) 6 0.44
7 2.99
8 5.10
9 83.11
10 0.95
Naphthenic Mono-Aromatic (NMAr) 9 2.40
Di-Aromatic (DiAr) 10 1.13
n-Olefin 10 0.54
n-Paraffin (n-P) 10 0.14
11 0.33
Total 97.12

The constituents that could fit in more than one HC block, or that did not fit in any specific block, were placed in a relevant block that presented the highest toxicity.
Analytical monitoring:
not required
Test organisms (species):
Eisenia fetida
Animal group:
annelids
Study type:
other: QSAR
Total exposure duration:
56 d
Reference substance (positive control):
no
Remarks:
Not Required
Duration:
14 d
Dose descriptor:
other: LL50
Effect conc.:
159 mg/kg soil dw
Nominal / measured:
estimated
Conc. based on:
other: QSAR prediction
Basis for effect:
mortality
Remarks on result:
other: QSAR prediction value
Duration:
56 d
Dose descriptor:
other: EL10
Effect conc.:
30.5 mg/kg soil dw
Nominal / measured:
estimated
Conc. based on:
other: QSAR prediction
Basis for effect:
reproduction
Remarks on result:
other: QSAR prediction value
Validity criteria fulfilled:
yes
Conclusions:
The QSAR predicted 14-d LL50 (mortality) and 56-d EL10 (reproduction) for E. fetida were 159 and 30.5 mg/kg dw respectively.
Executive summary:

The QSAR predicted 14-d LL50 (mortality) and 56-d EL10 (reproduction) of the UVCB substance for E. fetida  were 159 and 30.5 mg/kg soil dw, respectively. The model used for the calculations was the PETROTOX v4.0 model. PETROTOX is a well-documented and peer reviewed model that is widely used across the chemicals industry. This substance fits within the criteria of the model and there are no reservations about the validity of the model runs. It is expected that this data is reliable with restrictions.

Endpoint:
toxicity to soil macroorganisms except arthropods: long-term
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Justification for type of information:
See Attached Justification
Qualifier:
according to guideline
Guideline:
other: REACH Guideance on QSARs
Principles of method if other than guideline:
- Software tool(s) used including version:
See Field 'Attached justification'
- Model(s) used:
See Field 'Attached justification'
- Model description: See Field 'Attached justification'
- Justification of QSAR prediction: see field 'Attached justification'
GLP compliance:
not specified
Specific details on test material used for the study:
HC Block Type Carbon Number Concentration (%w/w)
Mono-Aromatic (MoAr) 7 1.55
8 31.91
9 17.86
10 2.89
12 2.29
13 0.67
Di-Aromatic (DiAr) 10 1.03
13 0.28
14 0.24
15 0.28
n-Olefin 5 0.27
10 21.06
11 0.41
i-Olefin 9 0.30
Total 81.03

The constituents that could fit in more than one HC block, or that did not fit in any specific block, were placed in a relevant block that presented the highest toxicity.
Analytical monitoring:
not required
Test organisms (species):
Eisenia fetida
Animal group:
annelids
Study type:
other: QSAR
Total exposure duration:
56 d
Reference substance (positive control):
no
Remarks:
Not Required
Duration:
14 d
Dose descriptor:
other: LL50
Effect conc.:
183 mg/kg soil dw
Nominal / measured:
estimated
Conc. based on:
other: QSAR prediction
Basis for effect:
mortality
Remarks on result:
other: QSAR prediction value
Duration:
56 d
Dose descriptor:
other: EL10
Effect conc.:
35 mg/kg soil dw
Nominal / measured:
estimated
Conc. based on:
other: QSAR prediction
Basis for effect:
reproduction
Remarks on result:
other: QSAR prediction value
Validity criteria fulfilled:
yes
Conclusions:
The QSAR predicted 14-d LL50 (mortality) and 56-d EL10 (reproduction) for E. fetida were 183 and 35.0 mg/kg dw respectively.
Executive summary:

The QSAR predicted 14-d LL50 (mortality) and 56-d EL10 (reproduction) of the UVCB substance for E. fetida were 183 and 35.0 mg/kg soil dw, respectively. The model used for the calculations was the PETROTOX v4.0 model. PETROTOX is a well-documented and peer reviewed model that is widely used across the chemicals industry. This substance fits within the criteria of the model and there are no reservations about the validity of the model runs. It is expected that this data is reliable with restrictions.

Endpoint:
toxicity to soil macroorganisms except arthropods: long-term
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Justification for type of information:
See Attached Justification
Qualifier:
according to guideline
Guideline:
other: REACH Guideance on QSARs
Principles of method if other than guideline:
- Software tool(s) used including version:
See Field 'Attached justification'
- Model(s) used:
See Field 'Attached justification'
- Model description: See Field 'Attached justification'
- Justification of QSAR prediction: see field 'Attached justification'
GLP compliance:
not specified
Specific details on test material used for the study:
HC Block Type Carbon Number Concentration (%w/w)
Mono-Aromatic (MoAr) 6 0.57
7 0.19
9 0.50
12 0.98
13 0.31
Di-Aromatic (DiAr) 10 0.27
n-Olefin 5 0.63
10 62.38
11 23.68
Total 89.52

The constituents that could fit in more than one HC block, or that did not fit in any specific block, were placed in a relevant block that presented the highest toxicity.
Analytical monitoring:
not required
Test organisms (species):
Eisenia fetida
Animal group:
annelids
Study type:
other: QSAR
Total exposure duration:
56 d
Reference substance (positive control):
no
Remarks:
Not Required
Duration:
14 d
Dose descriptor:
other: LL50
Effect conc.:
516 mg/kg soil dw
Nominal / measured:
estimated
Conc. based on:
other: QSAR prediction
Basis for effect:
mortality
Remarks on result:
other: QSAR prediction value
Duration:
56 d
Dose descriptor:
other: EL10
Effect conc.:
98.9 mg/kg soil dw
Nominal / measured:
estimated
Conc. based on:
other: QSAR prediction
Basis for effect:
reproduction
Remarks on result:
other: QSAR prediction value
Validity criteria fulfilled:
yes
Conclusions:
The QSAR predicted 14-d LL50 (mortality) and 56-d EL10 (reproduction) for E. fetida were 516 and 98.9 mg/kg dw respectively.
Executive summary:

The QSAR predicted 14-d LL50 (mortality) and 56-d EL10 (reproduction) of the UVCB substance for E. fetida were 516 and 98.9 mg/kg soil dw, respectively.The model used for the calculations was the PETROTOX v4.0 model. PETROTOX is a well-documented and peer reviewed model that is widely used across the chemicals industry. This substance fits within the criteria of the model and there are no reservations about the validity of the model runs. It is expected that this data is reliable with restrictions.

Endpoint:
toxicity to soil macroorganisms except arthropods: long-term
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Justification for type of information:
See Attached Justification
Qualifier:
according to guideline
Guideline:
other: REACH Guideance on QSARs
Principles of method if other than guideline:
- Software tool(s) used including version:
See Field 'Attached justification'
- Model(s) used:
See Field 'Attached justification'
- Model description: See Field 'Attached justification'
- Justification of QSAR prediction: see field 'Attached justification'
GLP compliance:
not specified
Specific details on test material used for the study:
HC Block Type Carbon Number Concentration (%w/w)
Mono-Aromatic (MoAr) 7 0.75
8 35.64
9 17.80
10 2.99
12 0.35
13 0.23
Di-Aromatic (DiAr) 10 0.22
n-Cyclohexane (n-CC6) 7 0.11
iso-Naphthenic (i-N) 8 0.10
n-Olefin 5 0.58
6 0.17
9 0.61
10 23.76
i-Olefin 6 0.21
n-Paraffin (n-P) 7 0.12
8 0.18
9 0.30
Total 84.13

The constituents that could fit in more than one HC block, or that did not fit in any specific block, were placed in a relevant block that presented the highest toxicity.
Analytical monitoring:
not required
Test organisms (species):
Eisenia fetida
Animal group:
annelids
Study type:
other: QSAR
Total exposure duration:
56 d
Reference substance (positive control):
no
Remarks:
Not Required
Duration:
14 d
Dose descriptor:
other: LL50
Effect conc.:
184 mg/kg soil dw
Nominal / measured:
estimated
Conc. based on:
other: QSAR prediction
Basis for effect:
mortality
Remarks on result:
other: QSAR prediction value
Duration:
56 d
Dose descriptor:
other: EL10
Effect conc.:
35.2 mg/kg soil dw
Nominal / measured:
estimated
Conc. based on:
other: QSAR prediction
Basis for effect:
reproduction
Remarks on result:
other: QSAR prediction value
Validity criteria fulfilled:
yes
Conclusions:
The QSAR predicted 14-d LL50 (mortality) and 56-d EL10 (reproduction) for E. fetida were 184 and 35.2 mg/kg dw respectively.
Executive summary:

The QSAR predicted 14-d LL50 (mortality) and 56-d EL10 (reproduction) of the UVCB substance for E. fetida were 184 and 35.2 mg/kg soil dw, respectively.The model used for the calculations was the PETROTOX v4.0 model. PETROTOX is a well-documented and peer reviewed model that is widely used across the chemicals industry. This substance fits within the criteria of the model and there are no reservations about the validity of the model runs. It is expected that this data is reliable with restrictions.

Endpoint:
toxicity to soil macroorganisms except arthropods: long-term
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Justification for type of information:
See Attached Justification
Qualifier:
according to guideline
Guideline:
other: REACH Guideance on QSARs
Principles of method if other than guideline:
- Software tool(s) used including version:
See Field 'Attached justification'
- Model(s) used:
See Field 'Attached justification'
- Model description: See Field 'Attached justification'
- Justification of QSAR prediction: see field 'Attached justification'
GLP compliance:
not specified
Specific details on test material used for the study:
HC Block Type Carbon Number Concentration (%w/w)
n-olefin 5 0.57
9 1.24
10 76.50
11 14.84
12 1.21
Total 94.37

The constituents that could fit in more than one HC block, or that did not fit in any specific block, were placed in a relevant block that presented the highest toxicity.
Analytical monitoring:
not required
Test organisms (species):
Eisenia fetida
Animal group:
annelids
Study type:
other: QSAR
Total exposure duration:
56 d
Reference substance (positive control):
no
Remarks:
Not Required
Duration:
14 d
Dose descriptor:
other: LL50
Effect conc.:
507 mg/kg soil dw
Nominal / measured:
estimated
Conc. based on:
other: QSAR prediction
Basis for effect:
mortality
Remarks on result:
other: QSAR prediction value
Duration:
56 d
Dose descriptor:
other: EL10
Effect conc.:
97.1 mg/kg soil dw
Nominal / measured:
estimated
Conc. based on:
other: QSAR prediction
Basis for effect:
reproduction
Remarks on result:
other: QSAR prediction value
Validity criteria fulfilled:
yes
Conclusions:
The QSAR predicted 14-d LL50 (mortality) and 56-d EL10 (reproduction) for E. fetida were 507 and 97.1 mg/kg dw respectively.
Executive summary:

The QSAR predicted 14-d LL50 (mortality) and 56-d EL10 (reproduction) of the UVCB substance for E. fetida  were 507 and 97.1 mg/kg soil dw for short-term, respectively. The model used for the calculations was the PETROTOX v4.0 model. PETROTOX is a well-documented and peer reviewed model that is widely used across the chemicals industry. This substance fits within the criteria of the model and there are no reservations about the validity of the model runs. It is expected that this data is reliable with restrictions.

Endpoint:
toxicity to soil macroorganisms except arthropods: long-term
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Justification for type of information:
See Attached Justification
Qualifier:
according to guideline
Guideline:
other: REACH Guideance on QSARs
Principles of method if other than guideline:
- Software tool(s) used including version:
See Field 'Attached justification'
- Model(s) used:
See Field 'Attached justification'
- Model description: See Field 'Attached justification'
- Justification of QSAR prediction: see field 'Attached justification'
GLP compliance:
not specified
Specific details on test material used for the study:
HC Block Type Carbon Number Concentration (%w/w)
Mono-Aromatic (MoAr) 8 1.51
9 42.57
10 7.04
Naphthenic Mono-Aromatic (NMAr) 9 0.98
10 0.36
Di-Aromatic (DiAr) 10 8.24
11 0.85
n-Olefin 5 0.22
9 0.13
10 26.65
11 4.67
Total 93.21

The constituents that could fit in more than one HC block, or that did not fit in any specific block, were placed in a relevant block that presented the highest toxicity.
Analytical monitoring:
not required
Test organisms (species):
Eisenia fetida
Animal group:
annelids
Study type:
other: QSAR
Total exposure duration:
56 d
Reference substance (positive control):
no
Remarks:
Not Required
Duration:
14 d
Dose descriptor:
other: LL50
Effect conc.:
198 mg/kg soil dw
Nominal / measured:
estimated
Conc. based on:
other: QSAR prediction
Basis for effect:
mortality
Remarks on result:
other: QSAR prediction value
Duration:
56 d
Dose descriptor:
other: EL10
Effect conc.:
37.9 mg/kg soil dw
Nominal / measured:
estimated
Conc. based on:
other: QSAR prediction
Basis for effect:
reproduction
Remarks on result:
other: QSAR prediction value
Validity criteria fulfilled:
yes
Conclusions:
The QSAR predicted 14-d LL50 (mortality) and 56-d EL10 (reproduction) for E. fetida were 198 and 37.9 mg/kg dw respectively.
Executive summary:

The QSAR predicted 14-d LL50 (mortality) and 56-d EL10 (reproduction) of the UVCB substance for E. fetida were 198 and 37.9 mg/kg soil dw, respectively. The model used for the calculations was the PETROTOX v4.0 model. PETROTOX is a well-documented and peer reviewed model that is widely used across the chemicals industry. This substance fits within the criteria of the model and there are no reservations about the validity of the model runs. It is expected that this data is reliable with restrictions.

Description of key information

The terrestrial toxicity was predicted by a QSAR, the PETROTOX v4.0 computer model. PETROTOX is a well-documented and peer reviewed model that is widely used across the chemicals industry. The substances of this category fit within the criteria of the model and there are no reservations about the validity of the model runs. The predicted LL50 values for Eisenia fetida ranged from 159 to 516 mg/kg soil dw. The predicted EL10 values for Eisenia fetida ranged from 30.5 to 98.9 mg/kg soil dw.

Key value for chemical safety assessment

Additional information

The PETROTOX v4.0 model for sediment terrestrial organism calculates the constituent concentration in sediment or soil using the product composition (% mass) along with the total sediment/soil (s/s) loading rate (concentration of the full substance as added to the soil/sediment). The model then uses the equilibrium-partitioning calculations to convert aqueous-based effect concentrations (EC50, mmol/L) to soil or sediment based values (mmol/kg oc) for each constituent using the CTLBB for the soil or sediment organism. Then, it combines the s/s based concentration (mg/kg dw) and the effect concentrations in soil or sediment based values (mmol/kg oc) to compute toxic units for each constituent, which are then summed to determine the overall effect concentration (LC50ss) of the substance.

 
The predicted values were:
Species Substance CAS No. LL50 / EL50 (mg/L) EL10 (mg/L)
Eisenia fetida 68516-20-1 184 35.2
Eisenia fetida 101316-62-5 160 30.6
Eisenia fetida 68526-56-7 507 97.1
Eisenia fetida 68477-54-3 183 35.0
Eisenia fetida 94733-07-0 198 37.9
Eisenia fetida 68478-10-4 516 98.9
Eisenia fetida 65996-79-4 159 30.5