reaction mass of: trisodium 3-(5-(2,6-difluoropyrimidin-4-ylamino)-2-sulfonatophenylazo)-5-(4-fluoro-6-morpholin-4-yl-1,3,5-triazin-2-ylamino)-4-hydroxy-2,7-naphthalenedisulfonate; trisodium 3-(5-(4,6-difluoropyrimidin-2-ylamino)-2-sulfonatophenylazo)-5-(4-fluoro-6-morpholin-4-yl-1,3,5-triazin-2-ylamino)-4-hydroxy-2,7-naphthalenedisulfonate

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1998-04-24 to 1998-08-03

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

GLP compliance:

yes

Type of method:

flask method

Water solubility:

ca. 581 g/L

Temp.:

30 °C

pH:

7.1

Water solubility:

ca. 389.3 g/L

Temp.:

30 °C

pH:

7.1

Remarks on result:

other: corrected for the purity of 67 % (w/w)

Table: Water solubility of the test substance

Experiment	1	2	3
Amount of test substance (g/L)	684.3	706.4	702.1
Stirring time at 30 °C	24	48	72
pH at room temperature	7.1	7.1	7.1
Water solubility	542.50	685.61	541.88

 

Average (experiments 1 to 3): 581.0 g/L

Standard deviation: 62.2 g/L

Relative standard deviation: 12 %

Purity-corrected value (67 % w/w): 389 g/L

 

Table: HPLC peak areas from the solubility experiment (dilution factor: 5000) and used standards.

Stirring time at 30 °C (h)	Peak areas (mV*min)	Concentrations (mg/l)	Peak areas (mV*min)
Experiment 1	Experiment 1	Standard	Standard
	92.767	50.476	83.706
24	96.994		92.854
Mean:	94.8805		88.2800
Experiment 2	Experiment 2	Standard	Standard
	123.162	50.940	92.242
48	120.035		95.858
Mean:	121.5985		94.0500

 Experiment 3 Experiment 3 Standard Standard    

106.528 56.512 114.046

72 108.449 110.152

Mean: 107.4885 112.0990                

Conclusions:

Interpretation of results (migrated information): very soluble (> 10000 mg/L)
The test substance Reactive Red FC 73270 is very soluble in water with a solubility of 581 g/L (purity-adjusted: 389 g/L) at 30°C and a pH of 7.1.

Executive summary:

The water solubility of the test substance was measured by HPLC in accordance with EU method A.6 and in compliance with GLP. The concentration of the substance was determined by comparison of HPLC peak area with an external standard.

The water solubility was determined to 581 g/l and corrected for the purity of 67 %, resulting in definitive solubility of 389 g/l (RSD: 12 %).

Description of key information

A study was performed to determine the water solubility of the test substance Reactive Red FC 73270 following EU method A.6 and in compliance with GLP, using the flask method and HPLC.

The test substance is classified as very soluble in water with a solubility of 581 g/L (389.3 g/L corrected for 67 % purity w/w) at 30°C at a pH of 7.1.

Key value for chemical safety assessment

Water solubility:

581 g/L

at the temperature of:

20 °C

Additional information

The water solubility of the test substance Reactive Red FC 73270 was measured by flask method in combination with HPLC and the concentration of the substance was determined by comparison of HPLC peak areas with an external standard.

The water solubility was determined to 581 g/l and corrected for the purity of 67 %, resulting in a definitive solubility of 389 g/l (RSD: 12%) at 30°C and a pH of 7.1. The test substance is classified as very soluble in water at the test conditions.