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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Reference
Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
May 2, 2013 - Dec. 31, 2013
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
no OECD GLP
Justification for type of information:
This study was performed according OECD TG 207. The quality system applied was not OECD GLP.
Qualifier:
according to guideline
Guideline:
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
GLP compliance:
no
Remarks:
no OECD GLP
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: L012138874
- Expiration date of the lot/batch: 2014-10-31
- Purity: > 99.92 %


Analytical monitoring:
no
Vehicle:
yes
Remarks:
Acetone
Details on preparation and application of test substrate:
Artificial soil test substrate was prepared as follows:
a) 10 per cent sphagnum peat (pH 6.0, no visible plant remains, finely ground, dried).
b) 20 per cent kaolin clay (kaolinite content preferably above 30 per cent).
c) 70 per cent industrial sand (fine sand should be dominant with 60 per cent of the
particles between 50 and 200 microns).
d) pH is adjusted to 6.0 by addition of calcium carbonate.
The dry constituents were blended in the correct proportions and mixed thoroughly with a
large-scale laboratory mixer.


Test subsance amount was calculated based on dry weight
Test organisms (species):
Eisenia fetida
Details on test organisms:
TEST ORGANISM
- Common name: Eisenia fetida
- Source: Earthworm nursery of Nanjing city in China
- Breeding generation: E20130415
- Age at test initiation (mean and range, SD): 2 month
- Weight at test initiation (mean and range, SD): 397 mg

ACCLIMATION
- Acclimation period: 24 h
- Acclimation conditions (same as test or not): no feeding
- Health during acclimation (any mortality observed): no mortality, no other findings
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
yes
Total exposure duration:
14 d
Test temperature:
20-22 °C
pH:
5.78 - 5.84
Moisture:
35% dry soil weight
Details on test conditions:
TEST SYSTEM
- Test container (material, size): 1 L glass vessel, closed
- Amount of soil or substrate: 750 g
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
- No. of replicates per vehicle control: 4



OTHER TEST CONDITIONS
- Photoperiod: 24 h/day
- Light intensity: 400 - 800 Lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
day 0 and 14

VEHICLE CONTROL PERFORMED: yes

TEST CONCENTRATIONS
1000 mg/kg dw
Nominal and measured concentrations:
1000 mg/kg dw
Reference substance (positive control):
no
Key result
Duration:
14 d
Dose descriptor:
LD50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
The mortalities of the blank control group, the solvent control group and the treatment group on day 14 were 0%.
Results with reference substance (positive control):
LD50 at about 25 mg/kg soil dw
Reported statistics and error estimates:
Standard statistical methods have been applied for data processing.
Validity criteria fulfilled:
yes
Conclusions:
Under conditions of the test, the 14 -day LD50 for the test substance to earthworms (Eisenia fetida) based on nominal test concentrations were higher than 1000 mg/kg dry soil weight.
Executive summary:

The informtion for this endpoint study record was obtained from an experimental study. The GLP (China) criteria were met and the methods applied are fully compliant with OECD TG 207. Under conditions of the test, the 14 -day LD50 for the test substance to earthworms (Eisenia fetida) based on nominal test concentrations were higher than 1000 mg/kg dry soil weight.

Description of key information

The informtion for this endpoint study record was obtained from an experimental study. The GLP (China) criteria were met and the methods applied are fully compliant with OECD TG 207. Under conditions of the test, the 14 -day LD50 for the test substance to earthworms (Eisenia fetida) based on nominal test concentrations were higher than 1000 mg/kg dry soil weight.

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:
1 000 mg/kg soil dw

Additional information